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Ectoine as a natural component of food: detection in red smear cheeses

Published online by Cambridge University Press:  26 October 2007

Julia Klein
Affiliation:
bitop AG, Stockumer Strasse 28, 58453 Witten, Germany
Thomas Schwarz
Affiliation:
bitop AG, Stockumer Strasse 28, 58453 Witten, Germany
Georg Lentzen*
Affiliation:
bitop AG, Stockumer Strasse 28, 58453 Witten, Germany
*
*For correspondence; e-mail: lentzen@bitop.de

Abstract

Ectoine is a compatible solute accumulated in halophilic bacteria in response to high salt concentrations and offers protection from osmotic stress. The occurrence of compatible solutes is widespread among bacteria, yet ectoine has never been detected in foods. The use of an ectoine producing microorganism (Brevibacterium linens) in the surface ripening of red smear cheeses led to the question whether ectoine can be found in cheese. Therefore we examined samples from a variety of cheese manufacturers and different types of red smear cheeses for the presence of ectoine using HPLC and HPLC/MS analysis. Ectoine solely appears in the rind and was detected up to 178 mg/200 g red smear cheese, depending on several factors like ripening status and conditions throughout the cheese production process (e.g. salt concentrations of used brine baths).

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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References

Bligh, EG & Dyer, WJ 1959 A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37 911917CrossRefGoogle ScholarPubMed
Bockelmann, W, Hoppe-Seyler, T & Heller, KJ 1996 Aroma and colour development of Tilsit cheeses. Kieler Milchwirtschaftliche Forschungsberichte 34Google Scholar
Bockelmann, W, Hoppe-Seyler, T, Jäger, B & Heller, KJ 2001 Defined Surface Cultures for Smeared Semi-Hard Cheese. Kieler Milchwirtschaftliche Forschungsberichte 146147Google Scholar
Bockelmann, W, Krusch, U, Engel, G, Klijn, N, Smit, G & Heller, KJ 1997 The microflora of Tilsit cheese. Part 1. Variability of the smear flora. Nahrung 41 208212CrossRefGoogle Scholar
Brown, AD 1976 Microbial water stress. Bacteriological Reviews 40 803846CrossRefGoogle ScholarPubMed
Bünger, J 1998 Neue Wirkstoffklasse schützt und pflegt die Haut. Parfümerie und Kosmetik 79 3235Google Scholar
Bünger, J 1999 Ectoin – Added protection and care for the skin. Euro Cosmetics 3 2224Google Scholar
Bünger, J, Degwert, J & Driller, H 2001 The protective function of compatible solute Ectoin on the skin, skin cells and its biomolecules with respect to UV-radiation, immunosupression and membrane damage. IFSSC Magazine 4 359365Google Scholar
Bünger, J & Driller, H 2004 Ectoin: an effective natural substance to prevent UVA-induced premature photoaging. Skin Pharmacology and Physiology 17 232237CrossRefGoogle Scholar
Corsetti, A, Rossi, J & Gobbetti, M 2001 Interactions between yeasts and bacteria in the smear surface-ripened cheeses. International Journal of Food Microbiology 19 110CrossRefGoogle Scholar
Feurer, C, Vallaeys, T, Corrieu, G & Irlinger, F 2004 Does Smearing Inoculum Reflect the Bacterial Composition of the Smear at the End of the Ripening of a French Soft, Red-Smear Cheese? Journal Dairy Sciences 87 31893197CrossRefGoogle ScholarPubMed
Galinski, EA, Pfeiffer, HP & Trüper, HG 1985 1,4,5,6-Tetrahydro-2-methyl-4-pyrimidinecarboxylic acid. A novel cyclic amino acid from halophilic phototrophic bacteria of the genus Ectothiorhodospira. European Journal of Biochemistry 149 135139CrossRefGoogle ScholarPubMed
Jäger, B, Hoppe-Seyler, T, Bockelmann, W & Heller, KJ 2000 Influence of Brine Microflora on the Ripening of Red Smear Cheeses. Kieler Milchwirtschaftliche Forschungsberichte 23Google Scholar
Jebbar, M, Champion, C, Blanco, C & Bonassie, S 1998 Carnitine acts as a compatible solute in Brevibacterium linens. Research in Microbiology 149 211219CrossRefGoogle ScholarPubMed
Lentzen, G & Schwarz, T 2006 Extremolytes: natural compounds from extremophiles for versatile applications. Applied Microbiology and Biotechnology 72 623634CrossRefGoogle ScholarPubMed
Lippert, K & Galinski, EA 1992 Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying. Applied Microbiology and Biotechnology 37 6165CrossRefGoogle Scholar
Loessner, M 2000 Listeria monocytogenes: Vorkommen in oberflächengereiften Weichkäsen und Entwicklung antagonistischer Reifungskulturen. PhD Thesis Technical University MünchenGoogle Scholar
Maoz, A, Mayr, R & Scherer, S 2003 Temporal Stability and Biodiversity of Two Complex Antilisterial Cheese-Ripening Microbial Consortia. Applied and Environmental Microbiology 69 40124018CrossRefGoogle ScholarPubMed
Margesin, R & Schinner, F 2001 Potential of halotolerant and halophilic microorganims for biotechnology. Extremophiles 5 7383CrossRefGoogle Scholar
Monnet, C, Correia, K, Sarthou, AS & Irlinger, F 2006 Quantitative Detection of Corynebacterium casei in Cheese by Real-Time PCR. Applied and Environmental Microbiology 72 69726979CrossRefGoogle ScholarPubMed
Mounier, J, Gelsomino, R, Goerges, S, Vancanneyt, M, Vandemeulebroecke, K, Hoste, B, Scherer, S, Swings, J, Fitzgerald, GF & Cogan, TM 2005 Surface microflora of four smear-ripened cheeses. Applied and Environmental Microbiology 71 64896500CrossRefGoogle ScholarPubMed
Mounier, J, Goerges, S, Gelsomino, R, Vancanneyet, M, Vandemeulebroecke, K, Hoste, B, Brennan, NM, Scherer, S, Swings, J, Fitzgerald, GF & Cogan, TM 2006 Sources of the adventitious microflora of a smear-ripened cheese. Journal of Applied Microbiology 101 668681CrossRefGoogle ScholarPubMed
Rattray, FP & Fox, PF 1999 Aspects of Enzymology and Biochemical Properties of Brevibacterium linens Relevant to Cheese Ripening: A Review. Journal of Diary Sciences 82 891909CrossRefGoogle ScholarPubMed
Roberts, MF 2005 Organic compatible solutes of halotolerant and halophilic microorganisms. Saline Systems 1 5CrossRefGoogle ScholarPubMed
Sauer, T & Galinski, EA 1998 Bacterial Milking: A Novel Bioprocess for Production of Compatible Solutes. Biotechnology and Bioengineering 57 3063133.0.CO;2-L>CrossRefGoogle ScholarPubMed
Severin, J, Wohlfarth, A & Galinski, EA 1992 The predominant role of recently discovered tetrahydropyrimidines for the osmoadaptation of halophilic eubacteria. Journal of General Microbiology 138 16291638CrossRefGoogle Scholar
Toolens, HP & Koning-Theune, W 1970 A selective medium for the detection of Brevbacterium linens in cheese. Milk Science International 25 7982Google Scholar