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Effect of different oxygen concentrations in the gas phase on biochemical activities of goat mammary tissue in organ culture

Published online by Cambridge University Press:  01 June 2009

Josef Škarda  and
Eva Urbanová
Josef Škarda
Affiliation:
Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences, 104 00 Prague, Czechoslovakia
Eva Urbanová
Affiliation:
Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences, 104 00 Prague, Czechoslovakia
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Summary

Non-secretory mammary expiants from virgin goats showed higher RNA and protein synthesis in a low O2 gas phase (air) than in high O2 (95% O2). Lipid and casein synthesis was not affected significantly by the concentration of O2 in the atmosphere during culture. on the other hand, the more developed mammary tissue from primigravid goats showed higher lipid, casein and protein synthesis in 95% O2. The relative response of mammary tissue to hormones was not substantially different when cultured in the presence of a low or high O2 gas phase. As Hepes-buffered medium was found not to need a supply of CO2 to maintain the correct pH and as Hepes did not interfere with biochemical activities of cells, it is recommended to use it for cultures in a low O2 gas phase.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 1 , February 1989 , pp. 1 - 5
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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