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The effect of long term under- and over-feeding on the expression of six major milk protein genes in the mammary tissue of sheep

Published online by Cambridge University Press:  01 July 2015

Eleni Tsiplakou ,
Emmanouil Flemetakis ,
Evangelia-Diamanto Kouri ,
George Karalias ,
Kyriaki Sotirakoglou  and
George Zervas
Eleni Tsiplakou*
Affiliation:
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece
Emmanouil Flemetakis
Affiliation:
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece
Evangelia-Diamanto Kouri
Affiliation:
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece
George Karalias
Affiliation:
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece
Kyriaki Sotirakoglou
Affiliation:
Department of Plant Breeding and Biometry, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece
George Zervas
Affiliation:
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece
*
*For correspondence; e-mail: eltsiplakou@aua.gr
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Abstract

Milk protein synthesis in the mammary gland involves expression of six major milk protein genes whose nutritional regulation remains poorly defined. In this study, the effect of long term under- and over-feeding on the expression of αs1-casein: CSN1S1, αs2-casein: CSN1S2, β-casein: CSN2, κ-casein: CSN3, α-lactalbumin: LALBA and β-lactoglobulin: BLG gene in sheep mammary tissue (MT) was examined. Twenty-four lactating dairy sheep, at 90–98 d in milk, were divided into three groups and fed the same ration, for 60 d, in quantities which met 70% (underfeeding), 100% (control) and 130% (overfeeding) of their energy and crude protein requirements. The results showed a significant reduction on mRNA of CSN1S1, CSN1S2, CSN2 and BLG gene in the MT of underfed sheep compared with the overfed ones and a significant reduction in CSN3 and LALBA gene expression compared with the respective control animals. Significant positive correlations were observed between the mRNA levels of milk proteins’ genes with the milk protein yield and milk yield respectively. In conclusion, the feeding level and consequently the nutrients availability, affected the milk protein yield and milk volume by altering the CSN1S1, CSN1S2, CSN2, CSN3, LALBA and BLG gene expression involved in their metabolic pathways.

Keywords

Underfeedingoverfeedingprotein genesheep
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 3 , August 2015 , pp. 257 - 264
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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