Bovine milk fat contains a low concentration of α-linolenic acid (cis-9, cis-12, cis-15 18:3), ranging from 0.5 to 2.0% of total fatty acids (FA; Kaylegian and Lindsay, Reference Kaylegian, Lindsay, Kaylegian and Lindsay1995; Lindmark Månsson, Reference Lindmark Månsson2008). The main reason for the low secretion of this n-3 FA in milk, despite its abundance in several feed ingredients, is the extensive biohydrogenation taking place in the rumen. Reviewing the literature, Doreau and Ferlay (Reference Doreau and Ferlay1994) reported a rate of hydrogenation of cis-9, cis-12, cis-15 18:3 ranging between 85 and 100%, with an average of 92%.
Under conditions of low FA intake, dietary cis-9, cis-12, cis-15 18:3 escaping ruminal biohydrogenation is selectively incorporated, after its absorption in the small intestine, into the plasma cholesterol esters (CE) and phospholipids (PL; Tyburczy et al., Reference Tyburczy, Lock, Dwyer, Destaillats, Mouloungui, Candy and Bauman2008). A limited portion is esterified to triacylglycerols (TAG) or remains in its free form. Less cis-9, cis-12, cis-15 18:3 is thus available for milk fat synthesis, as TAG are the primary sources of preformed FA for lipid synthesis in the mammary gland (Christie, Reference Christie and Christie1981). Such segregation process represents another obstacle in the transfer of dietary cis-9, cis-12, cis-15 18:3 to milk fat. However, as suggested by Christie (Reference Christie and Christie1981), when large amounts of polyunsaturated FA reach the intestines, the mechanism for segregating these components is exceeded and the surplus could be incorporated into TAG. In line with this premise, postruminal supply of cis-9, cis-12, cis-15 18:3, via abomasal infusion of linseed oil (L-oil), increased milk fat concentration of this n-3 FA up to 14.3% of milk fat (Fauteux et al., Reference Fauteux, Gervais, Rico, Lebeuf and Chouinard2016). This represents an apparent recovery of 27.7%, which is much greater than the efficiency of transfer of 1.95% reported in a meta-analysis (of 16 treatment means) by Leduc et al. (Reference Leduc, Létourneau-Montminy, Gervais and Chouinard2017) for dietary L-oil.
Despite this information, it appears that the quantitative aspect of the distribution mechanism of dietary polyunsaturated FA between plasma lipid classes and the resulting impact on their transfer efficiency into milk fat are not fully understood. The objective of our research was to determine the impact of postruminal supply of increasing amounts of L-oil, as a source of cis-9, cis-12, cis-15 18:3, on lactation performance, FA profile of plasma lipid fractions, and yield of milk FA. We hypothesised that a threshold value exists beyond which the postruminal supply of alpha-linolenic acid overcome the segregation mechanism generally observed during intestinal absorption and plasma transport, leading to a more even distribution of this fatty acid in varying lipid classes, and to an increased efficiency of its transfer to milk fat.
Materials and methods
Animals, feeding, and treatments
The experimental procedures involving dairy cows followed the guidelines of the Canadian Council on Animal Care (2009) and were approved by the Université Laval Animal Care Committee (Protocol # 2015001). The trial was conducted in an air-conditioned tie-stall facility at the Centre de Recherche en Sciences Animales de Deschambault, QC, Canada.
Five Holstein cows in early lactation (36 ± 2 d in milk; mean ± sd), weighing 732 ± 66 kg, and fitted with a rumen cannula (Ankom Technology, Macedon, NY, USA) were fed a total mixed ration based on corn and grass silages (online Supplemental Table S1) and formulated to meet or exceed energy and nutrient requirements (National Research Council, 2001). This total mixed ration was offered once daily at 10:00 h and the amount served was adjusted to obtain approximately 10% of refusals, ensuring ad libitum feeding conditions. Silages were sampled once a week and dried at 55°C for 72 h to determine their dry matter content and to adjust the proportions of feed ingredients in the total mixed ration on an as-fed basis. Free access to drinking water was available throughout the experiment.
Cows were randomly distributed in a 5 × 5 Latin square design with experimental periods of 21 d. During the first 14 d of each period, cows received an abomasal infusion of L-oil (Pokonobe Industries Inc., Westmount, QC; containing 5.6% 16:0, 3.4% 18:0, 18.4% cis-9 18:1, 0.7% cis-11 18:1, 14.9% cis-9, cis-12 18:2, 55.9% cis-9, cis-12, cis-15 18:3, and 0.2% 20:0) at the rate of 0, 75, 150, 300, and 600 ml/d. Oil was continuously delivered to the abomasum using peristaltic pumps (Flexiflo Patrol Pump; Abbott Nutrition Canada, Saint-Laurent, QC, Canada) connected to an infusion apparatus as described by Gressley et al. (Reference Gressley, Reynal, Olmos Colmenero, Broderick and Armentano2006). Each treatment period was followed by a 7-d washout interval.
Experimental measurements, samplings and analyses
Feed offered and refused was weighed and samples of the total mixed ration were collected from d 12 to 14 of each infusion period. Samples were dried at 55°C for 48 h to determine dry matter content and ground to 1 mm using a Wiley mill (model 4, Arthur H. Thomas Co., Philadelphia, PA). After being pooled by period, samples were analysed for residual moisture, neutral detergent fibre, acid detergent fibre, crude protein, and ash as described in the online Supplementary File, materials and methods. Determination of dietary FA composition was carried out by gas chromatography as described by Jenkins (Reference Jenkins2010) with modifications (Villeneuve et al., Reference Villeneuve, Lebeuf, Gervais, Tremblay, Vuillemard, Fortin and Chouinard2013).
Cows were milked twice daily at 7:00 and 17:00 h. Milk yield was measured using calibrated milk meters (Flomaster Pro, DeLaval, Tumba, Sweden), and samples were collected at each milking from d 12 to 14 on each infusion period. These samples were preserved with bronopol and stored at 4°C. At the end of each period, these samples were sent to a commercial laboratory (Lactanet, Ste-Anne-de-Bellevue, QC, Canada) where they were analysed for fat, protein, lactose, and urea-N concentrations by infrared absorption spectroscopy using a Foss MilkoScan FT 6000 (Foss, Hillerød, Denmark), and for somatic cell count determination using a Fossomatic FC (Foss). Yield of energy-corrected milk was calculated as described by Madsen et al. (Reference Madsen, Nielsen, Andersen and Ingvartsen2008). An additional set of milk samples without preservative were harvested during the last 3 d of each infusion and stored at −20°C for later determination of the FA profile as described in a companion paper (Rico et al., Reference Rico, Gervais, Peňa-Cotrino, Lebeuf and Chouinard2023 in press).
On d 13 of each infusion period, blood was withdrawn from the coccygeal vessel into evacuated plasma separation tubes containing EDTA (Vacutainer 366430, Becton Dickinson, Franklin Lakes, NJ, USA) at 09:30 (preprandial) and 15:30 (postprandial) h. Blood was placed on ice until centrifuged at 956 × g for 15 min and 4°C. The supernatant was transferred to microtubes and frozen at −20°C for further FA analysis of lipid classes (TAG, PL, CE, and FFA) as described in the online Supplementary File, materials and methods.
Statistical analysis
Data were analysed with the MIXED procedure of SAS (SAS Institute Inc., Cary, NC) as a 5 × 5 Latin square design according to the following model:
$$Y_{{\rm ijkl}}\,{\rm} = {\rm \mu }\,{\rm} + T_{\rm i}\,{\rm} + P_{\rm j}\,{\rm} + S_{\rm k}\,{\rm} + C_{\rm l}\,( S_{\rm k}) \,{\rm} + {\rm \varepsilon }_{{\rm ijkl}}$$where Y ijkl is the individual observation, μ the overall mean, T i the fixed effect of treatment (i = 1 to 5), P j the random effect of period (j = 1 to 5), S k the random effect of sequence (k = 1 to 5), C l(S k) the random effect of cow (l = 1–5) nested in sequence, and εijkl the residual error terms. Linear and quadratic contrasts for treatment effect were performed. Differences between treatments were declared at P ≤ 0.05.
Results
The online Supplementary File provides detailed data for dry matter intake, milk yield and composition (Table S2), intake, secretion and transfer efficiencies of individual FA (Table S3), FA yields (Table S4) and fatty acid profiles in TAG (Table S5), PL (Table S6), CE (Table S7) and FFA (Table S8) as well as the distribution of FA among plasma lipid classes (Table S9). Intake of the total mixed ration decreased linearly with increasing levels of L-oil infused into the abomasum (Fig. 1a). However, the estimated energy intake was not affected by treatments (Fig. 1b). Increasing levels of infusion linearly decreased the yield of energy-corrected milk (Fig. 1d). Milk fat concentration decreased quadratically, reaching a plateau at 300 ml of L-oil/d, whereas fat yield decreased linearly with increasing doses of L-oil (Fig. 1e and 1f). Milk protein and lactose concentrations remained stable, but protein yield decreased linearly with the level of oil infusion whereas lactose yield remained unchanged (Fig. 1g to 1j). Intake of cis-9, cis-12, cis-15 18:3 increased linearly with the level of infusion (Fig. 2a).
Figure. 1. Dry matter (a) and energy (b) intake, actual (c) and energy corrected (d) milk yield, and concentrations and yields of milk fat (e and f), protein (g and h) and lactose (i and j) in dairy cows abomasally infused with increasing levels of linseed oil. sem, standard error of the mean; L = linear and Q, quadratic effects of the level of linseed oil infusion. *P ≤ 0.05 and **P ≤ 0.01. NS, not significantly affected (P > 0.05). Table values can be found in online Supplementary File, Table S2.
Figure. 2. Intake (A), milk concentration (B) and secretion (C), and apparent transfer efficiency from diet to milk fat (D) of cis-9, cis-12, cis-15 18:3 in dairy cows abomasally infused with increasing levels of linseed oil. sem, standard error of the mean; L, linear and Q, quadratic effects of the level of linseed oil infusion. *P ≤ 0.05 and **P ≤ 0.01. Table values can be found in online Supplementary File, Tables S3 and S4.
Plasma concentrations of cis-9, cis-12, cis-15 18:3 circulating as TAG, PL and CE increased linearly and quadratically, whereas the concentration of this FA in its free form increased linearly with the amount of L-oil infused (Fig. 3, left panels). Similar variations were observed when concentrations of cis-9, cis-12, cis-15 18:3 were expressed in relative proportions of total FA in each of these plasma fractions (Fig. 3, right panels). Finally, when expressed as proportions of total circulating cis-9, cis-12, cis-15 18:3 (Fig. 4), concentrations of this FA increased linearly and quadratically in PL and TAG, increased linearly in FFA and decreased linearly and quadratically in CE.
Figure. 3. Concentrations (left panels) and relative proportion (right panels) of cis-9, cis-12, cis-15 18:3 in plasma triacylglycerols (TAG), phospholipids (PL), cholesterol esters (CE), and free fatty acids (FFA) fractions in dairy cows abomasally infused with increasing levels of linseed oil. sem, standard error of the mean; L, linear and Q, quadratic effects of the level of linseed oil infusion. *P ≤ 0.05 and **P ≤ 0.01. Table values can be found in online Supplementary File, Tables S5 to S8.
Figure. 4. Distribution of cis-9, cis-12, cis-15 18:3 among plasma lipid classes in dairy cows abomasally infused with increasing levels of linseed oil. sem, standard error of the mean; TAG, triacylglycerols; PL, phospholipids; CE, cholesterol esters, and FFA, free fatty acids; L, linear and Q, quadratic effects of the level of linseed oil infusion. *P ≤ 0.05 and **P ≤ 0.01. Table values can be found in online Supplementary File, Table S9.
Milk fat concentration of cis-9, cis-12, cis-15 18:3 increased linearly as the level of infusion of L-oil increased (Fig. 2b). Milk secretion and the transfer efficiency from diet + infusion to milk fat of cis-9, cis-12, cis-15 18:3 increased linearly and quadratically as the level of infusion of L-oil increased (Fig. 2c and 2d). Regarding the quadratic response of transfer efficiency, a plateau was reached at a daily infusion rate of 150 ml L-oil.
Discussion
Dairy cows were abomasally infused with L-oil in the current trial as a convenient method to avoid ruminal biohydrogenation of its constituent polyunsaturated FA. In a review, Benson et al. (Reference Benson, Reynolds, Humphries, Rutter and Beever2001) reported that abomasal infusion of L-oil decreased the intake of the total mixed ration. However, as the energy density of FA from L-oil is greater than that of the ingredients of the diet (National Research Council, 2001), lower dry matter intake was presumably a mechanism to maintain a constant ingestion of energy (Bull et al., Reference Bull, Baumgardt and Clancy1976). This satiety effect of abomasally infused polyunsaturated FA appears to be mediated through glucagon-like peptide-1 (7–36) amide (Litherland et al., Reference Litherland, Thire, Beaulieu, Reynolds, Benson and Drackley2005), products of pro-glucagon processing (pancreatic glucagon, gut glucagon, and glucagon-like peptide-1; Benson and Reynolds, Reference Benson and Reynolds2001) and cholecystokinin (Chelikani et al., Reference Chelikani, Glimm, Keisler and Kennelly2004), whereas leptin does not seem to be involved (Chelikani et al., Reference Chelikani, Glimm, Keisler and Kennelly2004).
Despite a lack of impact on energy intake, yield of energy-corrected milk decreased linearly with the amount of L-oil infused. The decrease in dry matter intake may have led to a shortage of nutrients to support the synthesis of major milk constituents (fat, protein and lactose). In addition to the impact on yield, milk fat concentration also decreased with increasing levels of L-oil infusion. A quadratic response was apparent with a 9% decrease observed at 300 ml of L-oil/d.
When cows received the control treatment (0 ml/d L-oil), 83.5% of plasma cis-9, cis-12, cis-15 18:3 were circulating as CE, 14.9% as PL, 0.8% as TAG and 0.6% as FFA (Fig. 4). This distribution differs somewhat from data reported by Tyburczy et al. (Reference Tyburczy, Lock, Dwyer, Destaillats, Mouloungui, Candy and Bauman2008; 66.3% as CE, 31.7% as PL, 1.7% as TG, and 0.3% as FFA) in cows in later lactation (259 ± 6 d post-partum) as compared to those used in the current trial (36 ± 2 d post-partum). Nevertheless, these results show that, at a low level of absorption, cis-9, cis-12, cis-15 18:3 is preferentially circulating as CE in blood plasma of dairy cows.
Concentrations of cis-9, cis-12, cis-15 18:3 increased quadratically in TAG, PL, and CE with level of infusion (Fig. 3). In these three fractions, a smaller slope was observed with an inflexion at an infusion rate of 300 ml of L-oil per day. The increase in plasma concentration of cis-9, cis-12, cis-15 18:3 was of a lower magnitude in CE as compared with the other two fractions. As a result, the relative proportion of cis-9, cis-12, cis-15 18:3 circulating as CE decreased quadratically as the level of L-oil infusion increased (Fig. 4). This observation is in line with the lower plasma proportion of cis-9, cis-12, cis-15 18:3 circulating as CE (66.3%) for a total concentration of 14.5 mg/dl in the experiment reported by Tyburczy et al. (Reference Tyburczy, Lock, Dwyer, Destaillats, Mouloungui, Candy and Bauman2008), as compared with the current experiments where 83.5% of plasma cis-9, cis-12, cis-15 18:3 was circulating as CE, for a total concentration of 9.4 mg/100 g.
The efficiency of transfer from diet + infusion to milk fat initially increased from 0 to 150 ml of L-oil infused per day and a plateau was maintained thereafter (Fig. 2d). This pattern resembles the quadratic response of the relative proportion of cis-9, cis-12, cis-15 18:3 circulating as TAG (Fig. 4), and the relative concentration of this FA in TAG (Fig. 3).
These results partly support our hypothesis as, by increasing the postruminal supply of cis-9, cis-12, cis-15 18:3, we were able to partly overcome the segregation mechanism of absorbed polyunsaturated FA in different plasma lipid classes. Proportionately more cis-9, cis-12, cis-15 18:3 was then esterified as TAG, at the expense of CE, increasing the efficiency of transfer into milk fat. This mechanism appeared to be surpassed in its turn when infusion increased over 150 ml/d, and transfer efficiency reached a plateau at greater supplies of L-oil. This observation is consistent with data reported by Baldwin et al. (Reference Baldwin, Smith, Taylor and Sharp1980) indicating that the relationship between plasma TAG concentration and mammary uptake is best described by Michaelis–Menten type kinetics. Nevertheless, the yield of cis-9, cis-12, cis-15 18:3 in milk fat continued to increase, but at a slower rate at the highest levels of infusion.
In conclusion, whilst ruminal biohydrogenation and selective incorporation into plasma CE and PL are two obstacles in the transfer of polyunsaturated FA form diet to milk fat in lactating ruminants, we were able to study the fate of postruminally available cis-9, cis-12, cis-15 18:3 by using abomasal infusion of L-oil. At low doses the transfer efficiency of this FA into milk fat gradually increased as compared with control. This observation was consistent with the parallel increase in relative proportion of cis-9, cis-12, cis-15 18:3 circulating as plasma TAG known to be the main source of preformed FA acid taken up by the mammary gland for milk fat synthesis. However, the transfer into milk fat of FA circulating as TAG is a saturable process, and the efficiency plateaued at greater levels of L-oil infusion. Nonetheless, the yield of cis-9, cis-12, cis-15 18:3 in milk fat continued to increase, but at a slower rate at the highest infusion rates. The transport of FA in blood plasma and their subsequent transfer to the mammary gland in dairy cows appear to be under a complex regulation which is not yet fully understood. A better comprehension of these mechanisms could help better predict the fate of essential FA in ruminants.
Supplementary material
The supplementary material for this article can be found at https://doi.org/10.1017/S0022029923000250
Acknowledgements
This experiment was funded through Industrial Research Chair program of the Natural Sciences and Engineering Research Council of Canada (Ottawa, ON, Canada), with industry contributions from the Dairy Farmers of Canada (Ottawa, ON, Canada), Novalait Inc. (Québec, QC, Canada), Valacta (Sainte-Anne-de-Bellevue, QC, Canada), Les Producteurs de Lait du Québec (Longueuil, QC, Canada), and the Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec (Québec, QC, Canada). The authors thank administrative and research staff of the Centre de Recherche en Sciences Animales de Deschambault (Deschambault, QC, Canada) for the care provided to cows during the trial.
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