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Effect of temperature on diacetyl and acetoin production by Lactococcus lactis subsp. lactis biovar diacetilactis CNRZ 483

Published online by Cambridge University Press:  01 June 2009

Naïma Bassit ,
Clair-Yves Boquien ,
Daniel Picque  and
Georges Corrieu
Naïma Bassit
Affiliation:
Laboratoire de Génie des Procédés Biotechnologiques Agro-alimentaires, Institut National de la Recherche Agronomique (INRA), F-78850 Thiverval-Grignon, France
Clair-Yves Boquien*
Affiliation:
Laboratoire de Génie des Procédés Biotechnologiques Agro-alimentaires, Institut National de la Recherche Agronomique (INRA), F-78850 Thiverval-Grignon, France
Daniel Picque
Affiliation:
Laboratoire de Génie des Procédés Biotechnologiques Agro-alimentaires, Institut National de la Recherche Agronomique (INRA), F-78850 Thiverval-Grignon, France
Georges Corrieu
Affiliation:
Laboratoire de Génie des Procédés Biotechnologiques Agro-alimentaires, Institut National de la Recherche Agronomique (INRA), F-78850 Thiverval-Grignon, France
*
*For correspondence
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Summary

We have investigated the effect of culture temperature on the growth of Lactococcus lactis subsp. lactis biovar diacetilactis, on its acidifying power, on diacetyl and acetoin production, and also on the activities of the principal enzymes involved in the synthesis of these two compounds. The rates of growth and lactic acid production decreased by a factor of ∼ 2 when the temperature decreased from 30 to 18°C. At 18°C, the maximal concentration of diacetyl (0·3mM) was 1·7 times that at 30°C, while that of acetoin was unchanged (5·2 mM). These results are explained by the behaviour of the principal enzymes involved in pyruvate metabolism. The activities of lactic dehydrogenase and acetolactate synthase varied little for culture temperatures between 18 and 30·C. However, the activity of NADH oxidase for a culture temperature of 18°C was 3·7 times that for 30°C, while that of diacetyl reductase for 30°C was 2·7 times that for 18°C. The net effect of temperature on these two activities was an increase in diacetyl production at lower temperature.

Type
Original articles
Information
Journal of Dairy Research , Volume 62 , Issue 1 , February 1995 , pp. 123 - 129
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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References

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