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Effects of intramammary inoculation of Lactobacillus perolens CRL1724 in lactating cows’ udders

Published online by Cambridge University Press:  14 November 2011

Ignacio D Frola ,
Matías S Pellegrino ,
María C Espeche ,
José A Giraudo ,
María EF Nader-Macias  and
Cristina I Bogni
Ignacio D Frola
Affiliation:
Department of Microbiology and Immunology, Faculty of Cs. Ex. Fco-Qcas y Naturales, University of Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
Matías S Pellegrino
Affiliation:
Department of Microbiology and Immunology, Faculty of Cs. Ex. Fco-Qcas y Naturales, University of Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
María C Espeche
Affiliation:
CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Cientifícas y Técnicas de Argentina), Departament of Preventive Microbiology, Chacabuco 145, 4000 San Miguel de Tucumán, Argentina
José A Giraudo
Affiliation:
Departament of Animal Pathology, Faculty of Agronomy and Veterinary, University of Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
María EF Nader-Macias
Affiliation:
CERELA-CONICET (Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Cientifícas y Técnicas de Argentina), Departament of Preventive Microbiology, Chacabuco 145, 4000 San Miguel de Tucumán, Argentina
Cristina I Bogni*
Affiliation:
Department of Microbiology and Immunology, Faculty of Cs. Ex. Fco-Qcas y Naturales, University of Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina
*
*For correspondence; e-mail: cbogni@exa.unrc.edu.ar
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Abstract

Bovine mastitis is the most important infectious disease on dairy farms. Conventional antibiotic therapy is often unsatisfactory and alternative treatments are continually under investigation. Lactobacillus (Lb.) perolens CRL 1724 and Lactobacillus plantarum CRL 1716 were previously isolated from milk of dairy cows and selected according to their potential probiotic properties. In the present work the in-vitro capacity of Lactobacillus strains to adhere to bovine teat canal epithelial cells (BTCEC) and to inhibit and co-aggregate 14 mastitis-causing pathogens (MCPs) was investigated. The effect of Lb. perolens CRL 1724 after intramammary inoculation in lactating cows was evaluated through determination of clinical signs of mastitis, milk appearance, somatic cell counts and Lb. perolens CRL 1724 recovery from milk. Lb. perolens CRL 1724 was able to inhibit 12 of 14 MCPs (85·7%) in vitro, especially those considered to be major pathogens. In addition, Lb. perolens CRL 1724 co-aggregated with all of them. Lb. plantarum CRL 1716 was able to inhibit 7 of 14 MCPs (50%) in vitro and showed co-aggregation ability similar to Lb. perolens CRL 1724. Lb. perolens CRL 1724 showed a higher efficacy of adhesion to BTCEC (values of percentage of adhesion and adhesion index of 75% and 14·4, respectively) than Lb. plantarum CRL 1716 (37% and 7·4, respectively). Lb. perolens CRL 1724 was recovered from all mammary quarters and no clinical signs or teat damage were observed after the inoculation of 106 cfu/ml. The udders presented a normal aspect and there were no changes in the appearance of the milk. The results obtained will serve as the basis for further trials to evaluate the potential of Lb. perolens CRL 1724 to be included in a non-antibiotic formulation for the prevention of bovine mastitis.

Keywords

Bovine mastitisprobioticLactobacillus perolensLactobacillus plantarummilk
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 1 , February 2012 , pp. 84 - 92
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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