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Enteric-coated capsule containing β-galactosidase-loaded polylactic acid nanocapsules: enzyme stability and milk lactose hydrolysis under simulated gastrointestinal conditions

Published online by Cambridge University Press:  29 September 2014

Hongjun He ,
Xueting Zhang  and
Yan Sheng
Hongjun He*
Affiliation:
College of Life Sciences, Yantai University, Yantai 264005, PR China
Xueting Zhang
Affiliation:
College of Life Sciences, Yantai University, Yantai 264005, PR China
Yan Sheng
Affiliation:
College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, PR China
*
*For correspondence; e-mail: hemiles@163.com
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Abstract

In order to protect peroral β-galactosidase from being degraded and hydrolyse milk lactose efficiently in the environments of gastrointestinal tract, a double-capsule delivery system composed of enteric-coated capsule and polylactic acid (PLA) nanocapsules (NCs) was developed for encapsulation of β-galactosidase. β-galactosidase-loaded PLA NCs in the size range of 100–200 nm were prepared by a modified w1/o/w2 technique. During the encapsulation process, dichloromethane/ethyl acetate (1 : 1, v/v) as the solvent composition, high-pressure homogenisation (150 bar, 3 min) as the second emulsification method and polyvinyl alcohol or Poloxamer 188 as a stabiliser in the inner phase could efficiently improve the activity retention of β-galactosidase (>90%). Subsequently, the prepared NCs were freeze-dried and filled in a hydroxypropyl methylcellulose phthalate (HP55)-coated capsule. In vitro results revealed that the HP55-coated capsule remained intact in the simulated gastric fluid and efficiently protected the nested β-galactosidase from acidic denaturation. Under the simulated intestinal condition, the enteric coating dissolved rapidly and released the β-galactosidase-loaded PLA NCs, which exhibited greater stability against enzymatic degradation and higher hydrolysis ratio (∼100%) towards milk lactose than the free β-galactosidase. These results suggest that this double-capsule delivery system represents promising candidate for efficient lactose hydrolysis in the gastrointestinal tract.

Keywords

Lactose hydrolysisβ-galactosidasepolymeric nanocapsulesenteric-coated capsulesimulated gastrointestinal conditions
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 4 , November 2014 , pp. 479 - 484
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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