Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-28T21:12:52.541Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of the efficacy of systemic danofloxacin in the treatment of induced acute Escherichia coli bovine mastitis

Published online by Cambridge University Press:  04 August 2008

Bernard Poutrel ,
Michael R Stegemann ,
Olivier Roy ,
Francis Pothier ,
Nicola Tilt  and
Mark Payne-Johnson
Bernard Poutrel*
Affiliation:
Unité d'Infectiologie Animale et Santé Publique, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
Michael R Stegemann
Affiliation:
Pfizer Animal Health, Sandwich, Kent CT13 9NJ, UK
Olivier Roy
Affiliation:
Cebiphar, Route de Pernay, 37230 Fondettes, France
Francis Pothier
Affiliation:
1 Avenue des Thermes, 63400 Chamalières, France
Nicola Tilt
Affiliation:
Pfizer Animal Health, Sandwich, Kent CT13 9NJ, UK
Mark Payne-Johnson
Affiliation:
Pfizer Animal Health, Sandwich, Kent CT13 9NJ, UK
*
*For correspondence; e-mail: b.poutrel@wanadoo.fr
Get access Rights & Permissions [Opens in a new window]

Abstract

The objective was to evaluate the efficacy of a single dose of danofloxacin (6 mg/kg bodyweight) given by the intravenous route for the treatment of acute bovine mastitis induced by intra-cisternal infusion of an Escherichia coli strain (26 cfu into one rear quarter of each cow). Twenty-three Prim'Holstein lactating cows were inoculated. To be challenged, the mammary glands had to be productive, free of pathogenic bacteria, and with somatic cell counts (SCC) of <200 000 cells/ml. The cows were treated on an individual basis when predetermined criteria involving both systemic and local clinical signs were satisfied. Allocation to treatment, danofloxacin or negative saline control, was performed according to a randomized treatment allocation plan. Monitoring during a 21-d period after inoculation included individual clinical examination, bacteriological examination and determination of SCC. Esch. coli was isolated from the milk of all inoculated quarters at the first milking post-inoculation and, together with reference to the clinical scores; the challenge was considered to be successful in 20 of the 23 cows. On study day 7 bacteriological cure rates with danofloxacin and saline control were 89% (8/9) and 44% (4/9) respectively. On days 14 and 21 all milk samples that could be collected were negative for Esch. coli in both groups of animals. Beneficial statistically significant differences were found at the end of the observation period (days 19–21 post treatment) between cows treated with danofloxacin and saline for SCC (P=0·0091) and earlier in the study for milk production (P=0·0003) and udder inflammation (P=0·004). Obvious beneficial trends were recorded in the danofloxacin group for rectal temperature, milk quality, general behaviour and appetite. Danofloxacin-treated cows showed statistically significant lower local clinical scores and a more rapid return to pre-inoculation values. It was concluded that systemically administered danofloxacin is effective in terms of bacteriological results, milk production and both systemic and local signs when used in the treatment of induced acute Esch. coli mastitis. Danofloxacin hastens recovery and return to productivity compared with potential self cure.

Keywords

MastitisbovineEscherichia colitreatmentsystemicdanofloxacin
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 3 , August 2008 , pp. 310 - 318
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Burvenich, C, Van Merris, V, Mehrzad, J, Diez-Fraile, A & Duchateau, L 2003 Severity of E. coli mastitis is mainly determined by cow factors. Veterinary Research 34 521–564CrossRefGoogle ScholarPubMed
Cebra, CK, Garry, FB & Dinsmore, RP 1996 Naturally occurring acute coliform mastitis in Holstein cattle. Journal of Veterinary Internal Medicine 10 252–257CrossRefGoogle ScholarPubMed
Craven, N 1991a Antibiotic therapy for control of mastisis. Economic aspects. Societe Francaise de Buiâtzie, pp. 107–118Google Scholar
Craven, N 1991b Antibiotic therapy in mastitis control. Economics and future prospects. Mammites des vaches laitières. Paris 18–19 décembre 1991 107–118Google Scholar
Dosogne, H, Meyer, E, Sturk, A, van Loon, J, Massart-Leen, AM & Burvenich, C 2002 Effect of enrofloxacin treatment on plasma endotoxin during bovine Escherichia coli mastitis. Inflammation Research 51 201–205CrossRefGoogle ScholarPubMed
Erskine, RJ, Bartlett, PC, VanLente, JL & Phipps, CR 2002 Efficacy of systemic ceftiofur as a therapy for severe clinical mastitis in dairy cattle. Journal of Dairy Science 85 2571–2575CrossRefGoogle ScholarPubMed
Erskine, RJ, Tyler, JW, Riddell, MG Jr. & Wilson, RC 1991 Theory, use, and realities of efficacy and food safety of antimicrobial treatment of acute coliform mastitis. Journal of the American Veterinary Medicine Association 198 980–984CrossRefGoogle ScholarPubMed
Grandemange, E & Davot, J 2002 Field evaluation of the efficacy of marbofloxacin in the treatment of acute mastitis due to Gram-negative bacteria in the dairy cow. Cattle Practice 10 57–62Google Scholar
Hill, AW & Shears, AL 1979 Recurrent coliform mastitis in the dairy cow. Veterinary Record 105 299–301CrossRefGoogle ScholarPubMed
Hill, AW, Shears, AL & Hibbitt, KG 1978 The elimination of serum-resistant Escherichia coli from experimentally infected single mammary glands of healthy cows. Research in Veterinary Science 25 89–93CrossRefGoogle ScholarPubMed
Hoeben, BD, Monfardini, E, Burvenich, C & Hamann, J 2000 Treatment of acute Escherichia coli mastitis in cows with enrofloxacin: effect on clinical signs and chemiluminescence of circulating neutrophils. Journal of Dairy Research 67 485–502CrossRefGoogle ScholarPubMed
Hogan, J & Smith, LK 2003 Coliform mastitis. Veterinary Research 34 507–519CrossRefGoogle ScholarPubMed
Jones, GF & Ward, GE 1990 Evaluation of systemic administration of gentamicin for treatment of coliform mastitis in cows. Journal of the American Veterinary Medicine Association 197 731–735CrossRefGoogle ScholarPubMed
Kutila, T, Suojala, L, Lehtolainen, T, Saloniemi, H, Kaartinen, L, Tahti, M, Seppala, K & Pyörälä, S 2004 The efficacy of bovine lactoferrin in the treatment of cows with experimentally induced Escherichia coli mastitis. Journal of Veterinary Pharmacology and Theriology 27 197–202CrossRefGoogle ScholarPubMed
Lohuis, JA, Schukken, YH, Verheijden, JH, Brand, A & Van Miert, AS 1990 Effect of severity of systemic signs during the acute phase of experimentally induced Escherichia coli mastitis on milk production losses. Journal of Dairy Science 73 333–341CrossRefGoogle ScholarPubMed
Pyörälä, S, Kaartinen, L, Kack, H & Rainio, V 1994 Efficacy of two therapy regimens for treatment of experimentally induced Escherichia coli mastitis in cows. Journal of Dairy Science 77 453–461CrossRefGoogle ScholarPubMed
Pyörälä, SH & Pyörälä, EO 1998 Efficacy of parenteral administration of three antimicrobial agents in treatment of clinical mastitis in lactating cows: 487 cases (1989–1995). Journal of the American Veterinary Medicine Association 212 407–412CrossRefGoogle ScholarPubMed
Rantala, M, Kaartinen, L, Valimaki, E, Stryrman, M, Hiekkaranta, M, Niemi, A, Saari, L & Pyörälä, S 2002 Efficacy and pharmacokinetics of enrofloxacin and flunixin meglumine for treatment of cows with experimentally induced Escherichia coli mastitis. Journal of Veterinary Pharmacology and Theriology 25 251–258CrossRefGoogle ScholarPubMed
Riollet, C, Rainard, P & Poutrel, B 2000 Differential induction of complement fragment C5a and inflammatory cytokines during intramammary infections with Escherichia coli and Staphylococcus aureus. Clinical Diagnostics and Laboratory Immunology 7 161–167CrossRefGoogle ScholarPubMed
Sandholm, M, Kaartinen, L & Pyörälä, S 1990 Bovine mastitis – why does antibiotic therapy not always work? An overview. Journal of Veterinary Pharmacology and Theriology 13 248–260CrossRefGoogle Scholar
Schneider, M, Valle, M, Woehrle, F & Boisrame, B 2004 Pharmacokinetics of marbofloxacin in lactating cows after repeated intramuscular administrations and pharmacodynamics against mastitis isolated strains. Journal of Dairy Science 87 202–211CrossRefGoogle ScholarPubMed
Serieys, F, Raguet, Y, Goby, L, Schmidt, H & Friton, G 2005 Comparative efficacy of local and systemic antibiotic treatment in lactating cows with clinical mastitis. Journal of Dairy Science 88 93–99CrossRefGoogle ScholarPubMed
Shem-Tov, M, Rav-Hon, O, Ziv, G, Lavi, E, Glickman, A & Saran, A 1998 Pharmacokinetics and penetration of danofloxacin from the blood into the milk of cows. Journal of Veterinary Pharmacology and Theriology 21 209–213CrossRefGoogle ScholarPubMed
Shpigel, NY, Levin, D, Winkler, M, Saran, A, Ziv, G & Bottner, A 1997 Efficacy of cefquinome for treatment of cows with mastitis experimentally induced using Escherichia coli. Journal of Dairy Science 80 318–323CrossRefGoogle ScholarPubMed
Vancutsem, PM, Babish, JG & Schwark, WS 1990 The fluoroquinolone antimicrobials: structure, antimicrobial activity, pharmacokinetics, clinical use in domestic animals and toxicity. Cornell Veterinarian 80 173–186Google ScholarPubMed
Vangroenweghe, F, Duchateau, L & Burvenich, C 2004 Moderate inflammatory reaction during experimental Escherichia coli mastitis in primiparous cows. Journal of Dairy Science 87 886–895CrossRefGoogle ScholarPubMed
VICH 2001 International Co-operation on Harmonization of Technical Requirements for Registration of Veterinary Medicinal Products, Good Clinical Practice, June 2000 effective 01 July 2001. Available from http://www.emea.europa.eu/pdfs/vet/vich/059598en.pdfGoogle Scholar
Wenz, JR, Barrington, GM, Garry, FB, McSweeney, KD, Dinsmore, RP, Goodell, G & Callan, RJ 2001 Bacteremia associated with naturally occuring acute coliform mastitis in dairy cows. Journal of the American Veterinary Medicine Association 219 976–981CrossRefGoogle ScholarPubMed
Ziv, G 1980 Practical pharmacokinetic aspects of mastitis therapy – 2: practical & therapeutic applications. Veterinary Medicine Small Animal Clinic 75 469–474Google ScholarPubMed