Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T08:09:28.764Z Has data issue: false hasContentIssue false
  • English
  • Français

Examination of methionine stimulation of gene expression in dairy cow mammary epithelial cells using RNA-sequencing

Published online by Cambridge University Press:  07 May 2020

Xiaoming Hou ,
Minghui Jiang ,
Jinyu Zhou ,
Shuyuan Song ,
Feng Zhao  and
Ye Lin
Xiaoming Hou
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin150030, China
Minghui Jiang
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin150030, China
Jinyu Zhou
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin150030, China
Shuyuan Song
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin150030, China
Feng Zhao
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin150030, China
Ye Lin*
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin150030, China
*
Author for correspondence: Ye Lin, Email: linlu516@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

In this research communication, a cell model with elevated β-CASEIN synthesis was established by stimulating bovine mammary epithelial cells with 0.6 mM methionine, and the genome-wide gene expression profiles of methionine-stimulated cells and untreated cells were investigated by RNA sequencing. A total of 458 differentially expressed genes (DEGs; 219 upregulated and 239 downregulated) were identified between the two groups. Gene Ontology (GO) analysis showed that the two highest-ranked GO terms in ‘molecular function’ category were ‘binding’ and ‘catalytic activity’, suggesting that milk protein synthesis in methionine-stimulated cells requires induction of gene expression to increase metabolic activity. Kyoto Encyclopedia of Genes and Genomes analysis revealed that within the ‘environmental information processing’ category, the subcategory that is most highly enriched for DEGs was ‘signal transduction’. cGMP-PKG, Rap1, calcium, cAMP, PI3K-AKT, MAPK, and JAK-STAT are the pathways with the highest number of DEGs, suggesting that these signaling pathways have potential roles in mediating methionine-induced milk protein synthesis in bovine mammary epithelial cells. This study provides valuable insights into the physiological and metabolic adaptations in cells stimulated with methionine. Understanding the regulation of this transition is essential for effective intervention in the lactation process.

Keywords

Bovine mammary epithelial cellmethioninemilk protein synthesisRNA sequencing
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 226 - 231
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Appuhamy, JA, Bell, AL, Nayananjalie, WA, Escobar, J and Hanigan, MD (2011) Essential amino acids regulate both initiation and elongation of mRNA translation independent of insulin in MAC-T cells and bovine mammary tissue slices. Journal of Nutrition 141, 1209–1215.CrossRefGoogle ScholarPubMed
Boxer, RB, Stairs, DB, Dugan, KD, Notarfrancesco, KL, Portocarrero, CP, Keister, BA, Belka, GK, Cho, H, Rathmell, JC, Thompson, CB, Birnbaum, MJ and Chodosh, LA (2006) Isoform-specific requirement for Akt1 in the developmental regulation of cellular metabolism during lactation. Cell Metabolism 4, 475–490.CrossRefGoogle ScholarPubMed
Chen, CC, Boxer, RB, Stairs, DB, Portocarrero, CP, Horton, RH, Alvarez, JV, Birnbaum, MJ and Chodosh, LA (2010) Akt is required for Stat5 activation and mammary differentiation. Breast Cancer Research: BCR 12, R72.CrossRefGoogle ScholarPubMed
Duan, X, Lin, Y, Lv, H, Yang, Y, Jiao, H and Hou, X (2017) Methionine induces LAT1 expression in dairy cow mammary gland by activating the mTORC1 signaling pathway. DNA and Cell Biology 36, 1126–1133.CrossRefGoogle ScholarPubMed
Hanigan, MD, Crompton, LA, Bequette, BJ, Mills, JA and France, J (2002) Modelling mammary metabolism in the dairy cow to predict milk constituent yield, with emphasis on amino acid metabolism and milk protein production: model evaluation. Journal of Theoretical Biology 217, 311–330.CrossRefGoogle ScholarPubMed
Hicks, MJ, Hu, Q, Macrae, E and DeWille, J (2015) Mitogen-activated protein kinase signaling controls basal and oncostatin M-mediated JUNB gene expression. Molecular and Cellular Biochemistry 403, 115–124.CrossRefGoogle ScholarPubMed
Lin, Y, Lv, H, Jiang, M, Zhou, J, Song, S and Hou, X (2019) Functional analysis of the dairy cow mammary transcriptome between early lactation and mid-dry period. Journal of Dairy Research, 1–5.Google ScholarPubMed
Ma, YF, Batistel, F, Xu, TL, Han, LQ, Bucktrout, R, Liang, Y, Coleman, DN, Parys, C and Loor, JJ (2019) Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. Journal of Dairy Science 102, 4264–4274.CrossRefGoogle ScholarPubMed
Schoenberg, KM, Giesy, SL, Harvatine, KJ, Waldron, MR, Cheng, C, Kharitonenkov, A and Boisclair, YR (2011) Plasma FGF21 is elevated by the intense lipid mobilization of lactation. Endocrinology 152, 4652–4661.CrossRefGoogle ScholarPubMed
Zhou, Y, Akers, RM and Jiang, H (2008) Growth hormone can induce expression of four major milk protein genes in transfected MAC-T cells. Journal of Dairy Science 91, 100–108.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

Hou et al. supplementary material

Hou et al. supplementary material

Download Hou et al. supplementary material(PDF)
PDF 1.3 MB