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Folic acid supplemented goat milk has beneficial effects on hepatic physiology, haematological status and antioxidant defence during chronic Fe repletion

Published online by Cambridge University Press:  14 November 2014

María JM Alférez ,
Emilio Rivas ,
Javier Díaz-Castro ,
Silvia Hijano ,
Teresa Nestares ,
Miguel Moreno ,
Margarita S Campos ,
Jose A Serrano-Reina  and
Inmaculada López-Aliaga
María JM Alférez
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Emilio Rivas
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Javier Díaz-Castro
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Silvia Hijano
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Teresa Nestares
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Miguel Moreno
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Margarita S Campos
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Jose A Serrano-Reina
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
Inmaculada López-Aliaga*
Affiliation:
Department of Physiology, Faculty of Pharmacy, University of Granada and Institute of Nutrition and Food Technology, Granada 18071, Spain
*
*For correspondence; e-mail: milopez@ugr.es
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Abstract

The aim of the current study was to asses the effect of goat or cow milk-based diets, either normal or Fe-overloaded and folic acid supplement on some aspects of hepatic physiology, enzymatic antioxidant defence and lipid peroxidation in liver, brain and erythrocyte of control and anaemic rats after chronic Fe repletion. 160 male Wistar rats were placed on 40 d in two groups, a control group receiving normal-Fe diet and the Fe-deficient group receiving low Fe diet. Lately, the rats were fed with goat and cow milk-based diets during 30 d, with normal-Fe content or Fe-overload and either with normal folic or folic acid supplemented. Fe-overload increased plasma alanine transaminase and aspartate transaminase levels when cow milk was supplied. Dietary folate supplementation reduced plasma transaminases levels in animals fed goat milk with chronic Fe overload. A remarkable increase in the superoxide dismutase activity was observed in the animals fed cow milk. Dietary folate supplement lead to a decrease on the activity of this enzyme in all the tissues studied with both milk-based diets. A concomitant increment in catalase was also observed. The increase in lipid peroxidation products levels in rats fed cow milk with Fe-overload, suggest an imbalance in the functioning of the enzymatic antioxidant defence. In conclusion, dietary folate-supplemented goat milk reduces both plasma transaminases levels, suggesting a hepatoprotective effect and has beneficial effects in situation of Fe-overload, improving the antioxidant enzymes activities and reducing lipid peroxidation.

Keywords

Goat and cow milkfolic acid supplementationantioxidant enzymeslipid peroxidation: chronic iron repletion
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 1 , February 2015 , pp. 86 - 94
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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