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Generation of hydrogen peroxide by a low molecular weight compound in whey of Holstein dairy cows

Published online by Cambridge University Press:  21 April 2008

Senkiti Sakai ,
Takahiro Satow ,
Kazuhiko Imakawa  and
Kentaro Nagaoka
Senkiti Sakai*
Affiliation:
Department of Animal Breeding, The Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
Takahiro Satow
Affiliation:
Department of Cell Biology, The Graduate School of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa 252-8510, Japan
Kazuhiko Imakawa
Affiliation:
Department of Animal Breeding, The Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
Kentaro Nagaoka
Affiliation:
Department of Animal Breeding, The Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
*
*For correspondence; e-mail: asenkiti@mail.ecc.u-tokyo.ac.jp
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Abstract

Using an ultrafiltration membrane (molecular cut-off, 3000), low molecular weight compounds in bovine milk were collected (YM-3 filtrate). A hydrogen peroxide (H2O2)-like substance was generated in the YM-3 filtrate. This substance was undetected at 0 h, but increased in a time-dependent manner, peaking after 2 h of incubation at 38°C. After incubating the YM-3 filtrate with catalase and lactoperoxidase, the signal showing the presence of this substance disappeared. The substance was quantified using one chemiluminescence and three colorimetric H2O2 detection systems. In all systems, their estimates were within the same range. The amount of substance, as estimated by the chemiluminescence H2O2 detection system, was correlated with that estimated by the other three colorimetric systems (r=0·98, 0·95 and 0·87). The substance was eluted at the same position as H2O2 by gel filtration on Superdex 30. Thus, the substance had the same characteristics as H2O2. An H2O2-generating substance in either the YM-3 filtrate or whey had a molecular mass of about 600. In this study, we clarify that bovine milk is capable of generating H2O2 by utilizing a low molecular weight compound. Thus, we present a new type of H2O2-supplying system in bovine milk.

Keywords

Dairy cowhydrogen peroxide productionmilk wheychemiluminescence
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 3 , August 2008 , pp. 257 - 261
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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