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Growth-promoting effects of pepsin- and trypsin-treated caseinomacropeptide from bovine milk on probiotics

Published online by Cambridge University Press:  24 June 2014

Gilles Robitaille  and
Claude P Champagne
Gilles Robitaille*
Affiliation:
Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Quebec, J2S 8E3, Canada
Claude P Champagne
Affiliation:
Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Quebec, J2S 8E3, Canada
*
*For correspondence; e-mail: gilles.robitaille@agr.gc.ca
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Abstract

Probiotic Lactobacillus and Bifidobacterium species are generally fastidious bacteria and require rich media for propagation. In milk-based media, they grow poorly, and nitrogen supplementation is required to produce high bacterial biomass levels. It has been reported that caseinomacropeptide (CMP), a 7-kDa peptide released from κ-casein during renneting or gastric digestion, exhibits some growth-promoting activity for lactobacilli and bifidobacteria. During the digestive process, peptides derived from CMP are detected in the intestinal lumen The aim of this study was to evaluate the effects of peptic and tryptic digests of CMP on probiotic lactic acid bacteria growth in de Man, Rogosa and Sharpe broth (MRS) and in milk during fermentation at 37 °C under anaerobic conditions. The study showed that pepsin-treated CMP used as supplements at 0·5 g/l can promote the growth of probiotics even in peptone-rich environments such as MRS. The effect was strain-dependent and evident for the strains that grow poorly in MRS, with an improvement of >1·5 times (P<0·05) by addition of pepsin-treated CMP. Trypsin-treated CMP was much less efficient as growth promoter. Moreover, pepsin-treated CMP was effective in promoting the growth in milk of all probiotic lactic acid bacteria tested, with biomass levels being improved significantly, by 1·7 to 2·6 times (P<0·05), depending on the strain. Thus, supplementation of MRS and of milk with pepsin-treated CMP would be advantageous for the production of high biomass levels for Bifidobacteria and Lactobacilli.

Keywords

Caseinomacropeptidepepsintrypsingrowth-promoting factorprobiotic
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 3 , August 2014 , pp. 319 - 324
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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