Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-26T08:19:57.489Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification and verification of differentially expressed genes in yak mammary tissue during the lactation cycle

Published online by Cambridge University Press:  19 March 2020

Mao Yuan ,
Wei Xia ,
Xiaolei Zhang ,
Yongtao Liu  and
Mingfeng Jiang
Mao Yuan
Affiliation:
The Research Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, State Ethnic Affairs Commission and Ministry of Education, Chengdu, China
Wei Xia
Affiliation:
The Research Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu, China College of Life Science and Technology, Southwest Minzu University, Chengdu, China
Xiaolei Zhang
Affiliation:
College of Life Science and Technology, Southwest Minzu University, Chengdu, China
Yongtao Liu
Affiliation:
College of Life Science and Technology, Southwest Minzu University, Chengdu, China
Mingfeng Jiang*
Affiliation:
The Research Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu, China College of Life Science and Technology, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, State Ethnic Affairs Commission and Ministry of Education, Chengdu, China
*
Author for correspondence: MingFeng Jiang, Email: mingfengjiang@vip.sina.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Yaks (Bos grunniens) live primarily in the Qinghai-Tibetan plateau (altitude: 2000–5000 m). Their milk presents unusual characteristics, containing large amounts of solids including fat and protein, and it is, therefore, important to understand the genetic makeup of the yak. To identify potentially critical genes playing a role in yak mammary tissue from colostrum to mature milk phase of lactogenesis, the early lactation (colostrum) stage (ELS; day 1 after parturition) and mature lactation (milk) stage (MLS; day 15) were chosen for comparison. An ELS-specific cDNA library was established by suppression subtractive hybridization and 25 expressed sequence tags at ELS were identified by sequencing and alignment. To further confirm our results the expression levels of 21 genes during the lactation cycle were measured using quantitative real-time RT-PCR (qRT-PCR). The qRT-PCR results confirmed 9 significantly up-regulated genes at ELS vs. MLS in yak mammary tissue, in which the l-amino acid oxidase 1 (LAO1) and collagen, type I, alpha I (COL1A1) were the most significantly up-regulated. During the lactation cycle, the highest expression of some milk fat genes (i.e., XDH and FABP3) in yak mammary tissue appears earlier than that in dairy cow. Our data also indicate MYC potentially playing a central role through putative regulation of COL1A1, CD44, SPARC, FASN and GPAM.

Keywords

Differentially expressed genesearly lactation stagemammary tissueYak
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 158 - 165
Copyright
Copyright © Hannah Dairy Research Foundation 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Mao Yuan and Wei Xia contributed equally to this work.

References

Akers, RM (2006) Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. Journal of Dairy Science 89, 1222–1234.10.3168/jds.S0022-0302(06)72191-9CrossRefGoogle ScholarPubMed
Barrington, GM, Mcfadden, TB, Huyler, MT and Besser, TE (2001) Regulation of colostrogenesis in cattle. Livestock Production Science 70, 95–104.10.1016/S0301-6226(01)00201-9CrossRefGoogle Scholar
Bionaz, M and Loor, JJ (2007) Identification of reference genes for quantitative real-time PCR in the bovine mammary gland during the lactation cycle. Physiological Genomics 29, 312–319.CrossRefGoogle ScholarPubMed
Bionaz, M and Loor, JJ (2008) Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics 9, 366.CrossRefGoogle ScholarPubMed
Dang, AK, Kapila, S, Purohit, M and Singh, C (2009) Changes in colostrum of Murrah buffaloes after calving. Tropical Animal Health & Production 41, 1213–1217.CrossRefGoogle ScholarPubMed
Dhorne-Pollet, S, Robert-Granié, C, Aurel, MR and Marie-Etancelin, C (2012) A functional genomic approach to the study of the milking ability in dairy sheep. Animal Genetics 43, 199–209.10.1111/j.1365-2052.2011.02237.xCrossRefGoogle Scholar
Green, DE, Nocito, V and Ratner, S (1944) L-Amino acid oxidase of animal tissues. Journal of Biological Chemistry 155, 421–440.Google Scholar
Grolli, S, Accornero, P, Ramoni, R, Donofrio, G and Whitelaw, CBA (1997) Expression of c-myc is down-regulated as mouse mammary epithelial cells become confluent. Biochemical & Biophysical Research Communications 239, 566–569.CrossRefGoogle ScholarPubMed
Hasselaar, P and Sage, EH (1992) SPARC antagonizes the effect of basic fibroblast growth factor on the migration of bovine aortic endothelial cells. Journal of Cellular Biochemistry 49, 272.CrossRefGoogle ScholarPubMed
He, AX and Li, L (2004) Study on the relation between yak performance and ecological protection. In JC, Zhong, XD, ZI and ZH, Chen (eds), Book Study on the Relation Between yak Performance and Ecological Protection, Series Study on the Relation Between yak Performance and Ecological Protection. Chengdu: Sichuan Publishing Group, pp. 93–98.Google Scholar
Hebbard, L, Steffen, A, Zawadzki, V, Fieber, C, Howells, N, Moll, J, Ponta, H, Hofmann, M and Sleeman, J (2000) CD44 expression and regulation during mammary gland development and function. Journal of Cell Science 113, 2619–2630.Google ScholarPubMed
Jack, LJ and Mather, IH (1990) Cloning and analysis of cDNA encoding bovine butyrophilin, an apical glycoprotein expressed in mammary tissue and secreted in association with the milk-fat globule membrane during lactation. Journal of Biological Chemistry 265, 14481–14486.Google ScholarPubMed
Jiang, MF, Lee, JN, Bionaz, M, Deng, XY and Wang, Y (2016) Evaluation of suitable internal control genes for RT-qPCR in yak mammary tissue during the lactation cycle. PLoS ONE 11, e0147705.10.1371/journal.pone.0147705CrossRefGoogle ScholarPubMed
Kent, WJ (2002) BLAT – the BLAST-like alignment tool. Genome Research 12, 656–664.10.1101/gr.229202CrossRefGoogle ScholarPubMed
Klebanoff, SJ, Clem, WH and Luebke, RG (1966) The peroxidase-thiocyanate-hydrogen peroxide antimicrobial system. Biochimica et Biophysica Acta 117, 63–72.CrossRefGoogle ScholarPubMed
Knight, CH, Hillerton, JE, Teverson, RM and Winter, A (1992) Biopsy of the bovine mammary gland. British Veterinary Journal 148, 129–132.10.1016/0007-1935(92)90104-9CrossRefGoogle ScholarPubMed
Nagaoka, K, Zhang, H, Arakuni, M, Taya, K and Watanabe, G (2014) Low expression of the antibacterial factor L-amino acid oxidase in bovine mammary gland. Animal Science Journal = Nihon Chikusan Gakkaiho 85, 976–980.Google ScholarPubMed
Noel, A and Foidart, JM (1998) The role of stroma in breast carcinoma growth in vivo. Journal of Mammary Gland Biology & Neoplasia 3, 215–225.CrossRefGoogle ScholarPubMed
O'Connell, BC, Cheung, AF, Simkevich, CP, Tam, W, Ren, X, Mateyak, MK and Sedivy, JM (2003) A large scale genetic analysis of c-Myc-regulated gene expression patterns. Journal of Biological Chemistry 278, 12563–12573.10.1074/jbc.M210462200CrossRefGoogle ScholarPubMed
Rompaey, LV, Dou, W, Buijs, A and Grosveld, G (1999) Tel, a frequent target of leukemic translocations, induces cellular aggregation and influences expression of extracellular matrix components. Neoplasia (New York, N.Y.) 1, 526–536.10.1038/sj.neo.7900064CrossRefGoogle ScholarPubMed
Roy, R, Zaragoza, P, Rodellar, C, Gautier, M and Eggen, A (2005) Radiation hybrid and genetic linkage mapping of two genes related to fat metabolism in cattle: fatty acid synthase (FASN) and glycerol-3-phosphate acyltransferase mitochondrial (GPAM). Animal Biotechnology 16, 1–9.10.1081/ABIO-200044295CrossRefGoogle Scholar
Sazanov, AA, Malewski, T, Kamiński, S and Zwierzchowski, L (2006) Characterization of the CHORI-240 BAC clones containing the bovine CSN1S1, CSN2, STATH, CSN1S2 and CSN3 genes. Journal of Applied Genetics 47, 243–245.CrossRefGoogle ScholarPubMed
Stiles, BG, Sexton, FW and Weinstein, SA (1991) Antibacterial effects of different snake venoms: purification and characterization of antibacterial proteins from Pseudechis australis (Australian king brown or mulga snake) venom. Toxicon 29, 1129–1141.10.1016/0041-0101(91)90210-ICrossRefGoogle ScholarPubMed
Strange, R, Li, F, Saurer, S, Burkhardt, A and Friis, RR (1992) Apoptotic cell death and tissue remodelling during mouse mammary gland involution. Development 115, 49–58.Google ScholarPubMed
Sun, Y, Nonobe, E, Kobayashi, Y, Kuraishi, T, Aoki, F, Yamamoto, K and Sakai, S (2002) Characterization and expression of L-amino acid oxidase of mouse milk. Journal of Biological Chemistry 277, 19080–19086.CrossRefGoogle ScholarPubMed
Travers, MT, Barber, MC, Tonner, E, Quarrie, L, Wilde, CJ and Flint, DJ (1996) The role of prolactin and growth hormone in the regulation of casein gene expression and mammary cell survival: relationships to milk synthesis and secretion. Endocrinology 137, 1530–1539.10.1210/endo.137.5.8612482CrossRefGoogle ScholarPubMed
Weaver, DM, Tyler, JW, VanMetre, DC, Hostetler, DE and Barrington, GM (2000) Passive transfer of colostral immunoglobulins in calves. Journal of Veterinary Internal Medicine 14, 569–577.CrossRefGoogle ScholarPubMed
Wiener, G, Han, JL and Long, RJ (2003) The Yak, second edition. The regional office for Asia and the Pacific, Food and Agriculture Organization of the United Nations, Bangkok, Thailand.Google Scholar
Supplementary material: PDF

Yuan et al. supplementary material

Yuan et al. supplementary material

Download Yuan et al. supplementary material(PDF)
PDF 252.7 KB