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Identification of lipid synthesis and secretion proteins in bovine milk

Published online by Cambridge University Press:  16 January 2014

Jing Lu ,
Toon van Hooijdonk ,
Sjef Boeren ,
Jacques Vervoort  and
Kasper Hettinga
Jing Lu
Affiliation:
Dairy Science and Technology, PDQ group, Wageningen University, Bomenweg 2, 6700 EV, Wageningen, The Netherlands Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands
Toon van Hooijdonk
Affiliation:
Dairy Science and Technology, PDQ group, Wageningen University, Bomenweg 2, 6700 EV, Wageningen, The Netherlands
Sjef Boeren
Affiliation:
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands
Jacques Vervoort
Affiliation:
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands
Kasper Hettinga*
Affiliation:
Dairy Science and Technology, PDQ group, Wageningen University, Bomenweg 2, 6700 EV, Wageningen, The Netherlands
*
*For correspondence; e-mail: kasper.hettinga@wur.nl
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Abstract

Lactation physiology is a process that is only partly understood. Proteomics techniques have shown to be useful to help advance the knowledge on lactation physiology in human and rodent species but have not been used as major tools for dairy cows, except for mastitis. In this paper, advanced non-targeted proteomics techniques (Filter aided sample preparation and NanoLC-Orbitrap-MS/MS) were applied to study the milk fat globule membrane and milk serum fraction, resulting in the identification of 246 proteins. Of these, 23 transporters and enzymes were related to lipid synthesis and secretion in mammary gland and their functions are discussed in detail. The identification of these intracellular transporters and enzymes in milk provides a possibility of using milk itself to study lipid synthesis and secretion pathways. This full-scale scan of milk proteins by using non-targeted proteomic analysis helps to reveal the important proteins involved in lipid synthesis and secretion for further examination in targeted studies.

Keywords

Proteomicslipid synthesis and secretionMFGMmilk serum
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 1 , February 2014 , pp. 65 - 72
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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