Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-28T04:41:08.321Z Has data issue: false hasContentIssue false
  • English
  • Français

Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant

Published online by Cambridge University Press:  21 November 2012

Cecilia M Camussone ,
Carolina M Veaute ,
Carina Porporatto ,
Bror Morein ,
Iván S Marcipar  and
Luis F Calvinho
Cecilia M Camussone
Affiliation:
E.E.A. Rafaela, INTA, Santa Fe, Argentina Concejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Carolina M Veaute
Affiliation:
Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Carina Porporatto
Affiliation:
Concejo Nacional de Investigaciones Científicas y Técnicas, Argentina Instituto de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Córdoba, Argentina
Bror Morein
Affiliation:
Department of Clinical Virology, Uppsala University, Sweden
Iván S Marcipar
Affiliation:
Concejo Nacional de Investigaciones Científicas y Técnicas, Argentina Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
Luis F Calvinho*
Affiliation:
E.E.A. Rafaela, INTA, Santa Fe, Argentina Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina
*
*For correspondence; e-mail: lcalvinho@rafaela.inta.gov.ar
Get access Rights & Permissions [Opens in a new window]

Abstract

The shortcomings of Staphylococcus aureus vaccines to control bovine mastitis have been attributed to insufficient capacity of the vaccines to induce opsonizing antibodies and to stimulate cellular immune responses. Types of antigen, administration route and adjuvant used in a vaccine formulation have been identified as critical factors for the development of opsonic antibodies. Current commercially available vaccines for Staph. aureus bovine mastitis control are formulated with Al(OH)3 and oil-based adjuvants. The aim of this study was to evaluate the immune response of heifers immunized with a Staph. aureus CP5 whole cell vaccine formulated either with Al(OH)3 or ISCOMATRIX™. Twenty primigravid Holstein dairy heifers in the last trimester of gestation were immunized either with a vaccine formulated with ISCOMATRIX™ (n = 6), Al(OH)3 (n = 7), or saline solution (placebo) (n = 7). Immunization was carried out 38 and 10 d before calving. Heifers vaccinated with Staph. aureus adjuvanted with ISCOMATRIX™ responded with significantly higher levels of anti-bacterin and anti-CP5 IgG and IgG2 in sera than animals in the Al(OH)3 or control groups. Animals in the ISCOMATRIX™ group responded with significantly higher anti-bacterin specific IgG in whey than animals in the Al(OH)3 and control groups, detected from the first week post calving until 60 d of lactation. Sera from animals inoculated with Staph. aureus in ISCOMATRIX™, obtained 7 d post partum, significantly increased both the number of neutrophils ingesting bacteria and the number of bacteria being ingested by the neutrophils, compared with sera obtained from heifers vaccinated with Al(OH)3 or non-vaccinated controls. These features coupled to safety of the ISCOMATRIX™ formulation, warrant additional studies.

Keywords

MastitisStaphylococcus aureusimmunizationISCOMATRIX™
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 1 , February 2013 , pp. 72 - 80
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barrio, MB, Rainard, P, Gilbert, FB & Poutrel, B 2003 Assessment of the opsonic activity of purified bovine sIgA following intramammary immunization of cows with Staphylococcus aureus. Journal of Dairy Science 86 2884–2894Google Scholar
Burton, JL & Erskine, RJ 2003 Immunity and mastitis. Some new ideas for an old disease. Veterinary Clinics of North America: Food Animal Practice 19 1–45Google Scholar
Camussone, C, Rejf, P, Pujato, N, Schwab, A, Marcipar, I & Calvinho, L 2012 Genotypic and phenotypic detection of capsular polysaccharides in Staphylococcus aureus isolated from bovine intramammary infections in Argentina. Brazilian Journal of Microbiology (In press)CrossRefGoogle ScholarPubMed
Fattom, A, Schneerson, R, Szu, S, Vann, W, Shiloach, J, Karakawa, W& Robbins, J 1990 Synthesis and immunologic properties in mice of vaccines composed of Staphylococcus aureus type 5 and type 8 capsular polysaccharides conjugated to Pseudomonas aeruginosa exotoxin A. Infection and Immunity 58 2367–2374Google Scholar
Federation of Animal Sciences Societies (FASS) 1999 Guide for the Care and Use of Agricultural Animals in Agricultural Research and TeachingGoogle Scholar
Fournier, JM, Hannon, K, Moreau, M, Karakawa, WW, & Vann, WF 1987 Isolation of type 5 capsular polysaccharide from Staphylococcus aureus. Annales de l´Institut Pasteur Microbiology 138 561–567Google Scholar
Gilbert, FB, Poutrel, B & Sutra, L 1994 Immunogenicity in cows of Staphylococcus aureus type 5 capsular polysaccharide-ovoalbumin conjugate. Vaccine 12 369–374CrossRefGoogle ScholarPubMed
Guidry, AJ, Oliver, SP, Squiggins, KE, Erbe, EF, Dowlen, HH, Hambleton, CN & Berning, LM 1991 Effect of anticapsular antibodies on neutrophil phagocytosis of Staphylococcus aureus. Journal of Dairy Science 74 3360–3369Google Scholar
Guidry, AJ, O'Brien, CN, Oliver, SP, Dowlen, HH & Douglass, LW 1994 Effect of whole Staphylococcus aureus and mode of immunization on bovine opsonizing antibodies to capsule. Journal of Dairy Science 77 2965–2974CrossRefGoogle ScholarPubMed
Han, HR, Pak, S & Guidry, A 2000 Prevalence of capsular polysaccharide (CP) types of Staphylococcus aureus isolated from bovine mastitic milk and protection of S. aureus infection in mice with CP vaccine. Journal of Veterinary Medical Science 62 1331–1333Google Scholar
Lee, JW, O'Brien, CN, Guidry, AJ, Paape, MJ, Shafer-Weaver, KA & Zhao, X 2005 Effect of a trivalent vaccine against Staphylococcus aureus mastitis lymphocyte subpopulations, antibody production, and neutrophil phagocytosis. Canadian Journal of Veterinary Research 69 11–18Google Scholar
Luby, CD, Middleton, JR, Ma, J, Rinehart, CL, Bucklin, S, Kohler, C & Tyler, JW 2007 Characterization of the antibody isotype response in serum and milk of heifers vaccinated with a Staphylococcus aureus bacterin (Lysigin™). Journal of Dairy Research 74 239–246CrossRefGoogle ScholarPubMed
Middleton, JR 2008 Staphylococcus aureus antigens and challenges in vaccine development. Expert Review of Vaccines 7 805–815CrossRefGoogle ScholarPubMed
Middleton, JR, Ma, J, Rinehart, CL, Taylor, VN, Luby, CD & Steevens, BJ 2006 Efficacy of different Lysigin™ formulations in the prevention of Staphylococcus aureus intramammary infection in dairy heifers. Journal of Dairy Research 73 10–19Google Scholar
Middleton, JR, Luby, CD & Scott Adams, D 2009 Efficacy of vaccination against staphylococcal mastitis: a review and new data. Veterinary Microbiology 1 2192–2198Google Scholar
Morein, B & Bengtsson, KL 1999 Immunomodulation by iscoms, immune stimulating complexes. Methods 19 94–102Google Scholar
Morein, B, Villacrés-Eriksson, M, Sjölander, A & Bengtsson, KL 1996 Novel adjuvants and vaccine delivery systems. Veterinary Immunology and Immunopathology 54 373–384Google Scholar
Morein, B, Hu, KF & Abusugra, I 2004 Current status and potential application of ISCOMs in veterinary medicine. Advanced Drug Delivery Reviews 56 1367–1382Google Scholar
Morein, B, Blomqvist, G & Hu, K 2007a Immune responsiveness in the neonatal period. Journal of Comparative Pathology 137 27–31CrossRefGoogle ScholarPubMed
Morein, B, Bengtsson, KL, D´Hondt, E & Hu, KF 2007b New ISCOMS meet unsettled vaccine demands in vaccine adjuvants and delivery systems. In: Vaccine Adjuvants and Delivery Systems. pp. 191–222 (Ed. Manmohan, Sing). Hoboken, New Jersey: John Wiley & Sons, Inc.CrossRefGoogle Scholar
Murphy, K 2011 Janeway's Immunobiology, 8th edition. New York: Garland Sciences.Google Scholar
Neave, FK, Dodd, FH, Kingwill, RG & Westgarth, DR 1969 Control of mastitis in the dairy herd by hygiene and management. Journal of Dairy Science 52 696–707CrossRefGoogle ScholarPubMed
Nelson, L, Flock, JI, Höök, M, Lindberg, M, Müller, HP & Wädstrom, T 1991 Adhesins in staphylococcal mastitis as vaccine components. Flemish Veterinary Journal 62 111–115Google Scholar
O'Brien, CN, Guidry, AJ, Douglass, LW & Westhoff, DC 2001 Immunization with Staphylococcus aureus lysate incorporated into microspheres. Journal of Dairy Science 84 1791–1799Google Scholar
Oliver, SP, Gonzalez, RN, Hogan, JS, Jayarao, BM & Owens, WE 2004 Microbiological Procedures for the Diagnosis of Bovine Udder Infection and Determination of Milk Quality, 4th edition. Verona, WI, USA: National Mastitis CouncilGoogle Scholar
Paape, MJ, Bannerman, DD, Zhao, X & Lee, JW 2003 The bovine neutrophil: structure and function in blood and milk. Veterinary Research 34 597–627Google Scholar
Papadopoulou, G, Karagouni, E & Dotsika, E 1998 ISCOMs vaccine against experimental leishmaniasis. Vaccine 16 885–892Google Scholar
Pearse, MJ & Drane, D 2004 ISCOMATRIX adjuvant: a potent inducer of humoral and cellular immune responses. Vaccine 22 2391–2395CrossRefGoogle ScholarPubMed
Pereira, UP, Oliveira, DGS, Mesquita, LR, Costa, GM & Pereira, LJ 2011 Efficacy of Staphylococcus aureus vaccines for bovine mastitis: a systematic review. Veterinary Microbiology 148 117–124Google Scholar
Pellegrino, M, Giraudo, J, Raspanti, C, Odierno, L & Bogni, C 2010 Efficacy of immunization against bovine mastitis using a Staphylococcus aureus avirulent mutant vaccine. Vaccine 28 4523–4528Google Scholar
Pfaffl, MW, Horgan, GW & Dempfle, L 2002 Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 30 e36Google Scholar
Riollet, C, Rainard, P & Poutrel, B 2001 Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection. Journal of Dairy Science 84 1077–1084Google Scholar
Robson, NC, Beacock-Sharp, H, Donachie, AM & Mowat, AM 2003 The role of antigen-presenting cells and interleukin-12 in the priming of antigen-specific CD4 + T cells by immune stimulating complexes. Immunology 110 95–104Google Scholar
Siemsen, DW, Schepetkin, IA, Kirpotina, LN, Lei, B & Quinn, MT 2007 Neutrophil isolation from nonhuman species. Methods in Molecular Biology 412 21–34Google Scholar
Sjölander, A, Cox, JC & Barr, IG 1998 ISCOMs: an adjuvant with multiple functions. Journal of Leukocyte Biology 64 713–723Google Scholar
Smith, RE, Donachie, AM, Grdic, D, Lycke, N & Mowat, AM 1999 Immune-stimulating complexes induce an IL-12-dependent cascade of innate immune responses. Journal of Immunology 162 5536–5546Google Scholar
Sordillo, LM & Streicher, KL 2002 Mammary gland immunity and mastitis susceptibility. Journal of Mammary Gland Biology and Neoplasia 7 135–146Google Scholar
Sun, HX, Xie, Y & Ye, YP 2009 ISCOMs and ISCOMATRIX. Vaccine 27 4388–4401Google Scholar
Tollersrud, T, Kenny, K, Reitz, AJ & Lee, JC 2000 Genetic and serologic evaluation of capsule production by bovine mammary isolates of Staphylococcus aureus and other Staphylococcus spp. From Europe and the United States. Journal of Clinical Microbiology 38 2998–3003Google Scholar
Tollersrud, T, Zernichow, L, Andersen, SR, Kenny, K & Lund, A 2001 Staphylococcus aureus capsular polysaccharide type 5 conjugate and whole cell vaccines stimulate antibody responses in cattle. Vaccine 19 3896–3903Google Scholar
Wallemacq, H, Bedoret, D, Pujol, J, Desmet, C, Drion, PV, Farnir, F, Mainil, J, Lekeux, P, Bureau, F & Fiévez, L 2012 CD40 triggering induces strong cytotoxic T lymphocyte responses to heat-killed Staphylococcus aureus immunization in mice: a new vaccine strategy for staphylococcal mastitis. Vaccine 30 2116–2124Google Scholar
Weingart, CL, Broitman-Maduro, G, Dean, G, Newman, S, Peppler, M & Weiss, AA 1999 Fluorescent labels influence phagocytosis of Bordetella pertussis by human neutrophils. Infection and Immunity 67 4264–4267CrossRefGoogle ScholarPubMed
Zecconi, A, Calvinho, LF & Fox, KL 2006 Stapylococcus aureus intramammary infections. IDF Bulletin 408 1–36Google Scholar
Zetterlund, A, Larsson, PH, Müller-Suur, C, Palmberg, L & Larsson, K 1998 Budesonide but not terbutaline decreases phagocytosis in alveolar macrophages. Respiratory medicine 92 162–166CrossRefGoogle Scholar
Zhang, G, Young, JR, Tregaskes, CA, Sopp, P & Howard, CJ 1995 Identification of a novel class of mammalian Fc gamma receptor. Journal of Immunology 155 1534–1541Google Scholar
Zhu, J, Yamane, H & Paul, WE 2010 Differentiation of effector CD4T cell opulations. Annual Review of Immunology 28 445–489Google Scholar