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Influence of bovine and caprine casein phosphopeptides differing in αs1-casein content in determining the absorption of calcium from bovine and caprine calcium-fortified milks in rats

Published online by Cambridge University Press:  26 July 2007

Adela Mora-Gutierrez ,
Harold M Farrell Jr ,
Rahmat Attaie ,
Velva J McWhinney  and
Changzheng Wang
Adela Mora-Gutierrez*
Affiliation:
Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, USA
Harold M Farrell Jr
Affiliation:
Eastern Regional Research Center, Agricultural Research Center, USDA, Wyndmoor, PA 19038, USA
Rahmat Attaie
Affiliation:
Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, USA
Velva J McWhinney
Affiliation:
Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, USA
Changzheng Wang
Affiliation:
Human Nutrition Research, Kentucky State University, Frankfort, KY 40601, USA
*
*For correspondence; e-mail: admora@pvamu.edu
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Abstract

Bovine and caprine milks have a similar overall gross composition, but vary considerably in the ratios of their casein components. These differences cause significant changes in the ability of caseins to bind and stabilize calcium (Ca). It might be expected that these in vitro variations, which are thought to be due to differences in casein phosphopeptides (CPP) content, could lead to in vivo differences in the digestion and absorption of Ca. To test this hypothesis three milks with different casein ratios [bovine (B), caprine high in αs1-casein (CH) and caprine low in αs1-casein (CL)] were compared with regard to Ca absorption and deposition in growing male rats. For comparison, each milk was Ca-fortified (BCa-milk, CHCa-milk, and CLCa-milk) and CPP, prepared by enzymatic hydrolysis from the respective caseins (extrinsic CPP), were added to both native and Ca-milks. The effects of added CPP (extrinsic) could then be compared with intrinsic CPP released from the gastrointestinal digestion of caseins. Total gastric Ca was sampled at 15, 30 and 60 min after ingestion. No differences were found among the native milks with or without CPP, but the Ca from all Ca-milks (regardless of casein type) appeared to clear the stomach more rapidly and this was enhanced by the extrinsic CPP. The total intestinal Ca was not different among the native milks±CPP, however, it rose more rapidly with Ca fortification, and was higher at 30 min for all CPP-Ca-milks. At 60 min the total intestinal Ca level fell for the CPP-Ca-milks while all others continued to rise. These observations suggest that the CPP in Ca-milks enhance gastric clearance and uptake from the intestine. Ca availability from BCa-milk, CHCa-milk, and CLCa-milk with and without CPP was estimated by both plasma and femur uptake of 45Ca. Ca availability was enhanced at 5 h in the plasma in each case by added CPP. In all cases CPP stimulated Ca availability in the femur, but the CL-CPP was higher (P<0·05) than that of either CH-CPP or B-CPP (extrinsic CPP). Based on the results of this study we can conclude that the addition of CPP will have beneficial effect on the absorption of Ca in growing rats from CaCO3 added to bovine and caprine milks.

Keywords

calcium absorptioncasein phosphopeptidesbovinecaprinemilk
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 3 , August 2007 , pp. 356 - 366
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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References

Ambrosoli, R, Di Stasio, L & Mazzocco, P 1988 Content of αs1-casein and coagulation properties in goat milk. Journal of Dairy Science 71 24–28CrossRefGoogle Scholar
American Institute of Nutrition 1977 Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107 1340–1348CrossRefGoogle Scholar
AOAC Official Methods of Analysis of the AOAC 1995 15th ed. Assoc. Official Analytical Chemists, Arlington, VA.Google Scholar
Aoki, T, Yamada, N, Kako, Y & Imamura, T 1988 Dissociation during dialysis of casein aggregates cross-linked by colloidal calcium phosphate in bovine casein micelles. Journal of Dairy Research 55 189–195CrossRefGoogle Scholar
Bak, M, Rasmussen, LK, Petersen, TE & Nielsen, NC 2001 Colloidal calcium phosphates in casein micelles studied by slow-speed-spinning 31P magic angle spinning solid-state nuclear magnetic resonance. Journal of Dairy Science 84 1310–1319CrossRefGoogle ScholarPubMed
Basch, JJ, Douglas, FW Jr, Procino, LG, Holsinger, VH & Farrell, HM Jr 1985 Quantitation of caseins and whey proteins of processed milks and whey protein concentrates, applications of gel electrophoresis, and comparison with Harland-Ashworth procedure. Journal of Dairy Science 68 23–31CrossRefGoogle Scholar
Basch, JJ, Farrell, HM Jr, Walsh, R, Konstance, R & Kumosinski, TF 1989 Development of a quantitative model for enzyme-catalyzed time-dependent changes in protein composition of Cheddar cheese during storage. Journal of Dairy Science 72 591–603CrossRefGoogle Scholar
Berner, LA, McBean, LD & Lofgren, PA 1990 Calcium and chronic disease prevention: Challenges to the food industry. Food Technology 3 50–70Google Scholar
Biological Council 1987 Guidelines on the Use of Living Animals in Scientific Investigations. London, UKGoogle Scholar
Buchowski, MS, Sowizral, KC, Lengemann, FW, Van Campen, D & Miller, DD 1989 A comparison of intrinsic and extrinsic tracer methods for estimating calcium bioavailability to rats from dairy foods. Journal of Nutrition 119 228–234CrossRefGoogle ScholarPubMed
Buchowski, MS & Miller, DD 1990 Calcium bioavailability from ripening cheddar cheese. Journal of Food Science 55 1293–1295CrossRefGoogle Scholar
Cámara-Martos, F & Amaro-López, MA 2002 Influence of dietary factors on calcium bioavailability: a brief review. Biological Trace Elements Research 89 43–52CrossRefGoogle ScholarPubMed
Cerbulis, J & Farrell, HM Jr 1976 Composition of milks of dairy cattle. II. Ash, calcium, magnesium, and phosphorus. Journal of Dairy Science 59 589–593CrossRefGoogle Scholar
Combes, C, Patterson, E & Amadò, R 2002 Isolation and identification of low-molecular-weight peptides from Emmentaler cheese. Journal of Food Science 67 553–559CrossRefGoogle Scholar
Du, X, Zhu, K, Trube, A, Zhang, Q, Ma, G, Hu, X, Fraser, D & Greenfield, H 2004 School-milk intervention trial enhances growth and bone mineral accretion in Chinese girls aged 10–12 years in Beijing. British Journal of Nutrition 92 159–168CrossRefGoogle ScholarPubMed
Erba, D, Ciappellano, S & Testolin, G 2002 Effect of ratio of casein phosphopeptides to calcium (w/w) on passive calcium transport in the distal small intestine of rats. Nutrition 18 743–746CrossRefGoogle ScholarPubMed
Farrell, HM Jr 1999. Milk composition and synthesis. In Encyclopedia of Reproduction, pp. 256–263 (Eds Knobil, E & Neil, JD). San Diego: Academic Press, Inc.Google Scholar
Farrell, HM Jr, Jimenez-Flores, R, Bleck, GT, Brown, EM, Butler, JE, Creamer, LK, Hicks, CL, Hollar, CM, Ng-Kwai-Hang, KF & Swaisgood, HE 2004 Nomenclature of the proteins of cows' milk-sixth revision. Journal of Dairy Science 87 1641–1674CrossRefGoogle ScholarPubMed
Farrell, HM Jr, Malin, EL, Brown, EM & Qi, PX 2006 Casein micelle structure: what can be learned from milk synthesis and structural biology? Journal of Colloid and Interface Science 11 135–147Google Scholar
Favus, MJ 1985 Factors that influence absorption and secretion of calcium in the small intestine and colon. American Journal of Physiology 248 G147–G157Google ScholarPubMed
Fisher, JO, Mitchell, DC, Smiciklas-Wright, H, Mannino, M & Birch, LL 2004 Meeting calcium recommendations during middle childhood reflects mother-daughter beverage choices and predicts bone mineral status. American Journal of Clinical Nutrition 79 698–706CrossRefGoogle ScholarPubMed
Hansen, M, Sandström, B, Jensen, M & Sørensen, S 1997 Casein phosphopeptides improve zinc and calcium absorption from rice-based but not from whole-grain infant cereal. Journal of Pediatric Gastroenterology and Nutrition 24 56–62Google Scholar
Heaney, RP, Saito, Y & Orimo, H 1994 Effect of casein phosphopeptide on absorbability of co-ingested calcium in normal postmenopausal women. Journal of Bone Mineral Metabolism 12 77–81CrossRefGoogle Scholar
Hirayama, M, Toyota, K, Yamaguchi, G, Hidaka, H & Naito, H 1992 HPLC analysis of commercial casein phosphopeptides (CPP). Bioscience, Biotechnology, and Biochemistry 56 1126–1127CrossRefGoogle ScholarPubMed
Infante, D & Tormo, R 2000 Risk of inadequate bone mineralization in diseases involving long-term suppression of dairy products. Journal of Pedriatic and Gastric. Nutrition 30 310–313Google ScholarPubMed
Kärkkäinen, MU, Wiersma, JW & Lamberg-Allardt, CJ 1997 Postprandial parathyroid hormone response to four calcium-rich foodstuffs. American Journal Clinical Nutrition 65 1726–1730CrossRefGoogle ScholarPubMed
Kato, K, Takada, Y, Matsuyama, H, Kawasaki, Y, Aoe, S, Yano, H & Toba, Y 2002 Milk calcium taken with cheese increases bone mineral density and bone strength in growing rats. Bioscience, Biotechnology, and Biochemistry 66 2342–2346CrossRefGoogle ScholarPubMed
Kitts, D 1994 Bioactive substances in food: identification and potential uses. Canadian Journal of Physiology and Pharmacology 72 423–434CrossRefGoogle ScholarPubMed
Lee, YS, Park, G & Naito, H 1992 Supplemental effect of casein phosphopeptides on the calcium balance of growing rats. Journal of Japanese Society of Nutrition and Food Science 45 333–338CrossRefGoogle Scholar
López-Huertas, E, Teucher, B, Boza, JJ, Martínez-Feréz, A, Majsak-Newman, G, Baró, L, Carrero, JJ, González-Santiago, M, Fonollá, J & Fairweather-Tait, S 2006 Absorption of calcium from milks enriched with fructo-oligosaccharides, caseinophosphopeptides, tricalcium phosphate, and milk solids. American Journal of Clinical Nutrition 83 310–316CrossRefGoogle ScholarPubMed
Marin, J & Zee, JA 1992 Influence of hydrocolloids on potential bioavailability of calcium in dairy products. Microbiologie Aliments Nutrition 10 23–28Google Scholar
Matkovic, V, Landoll, JD, Badenhop-Stevens, NE, Ha, EY, Crncevic-Orlic, Z, Li, B & Goel, P 2004 Nutrition influences skeletal development from childhood to adulthood: a study of hip, spine, and forearm in adolescent females. Journal of Nutrition 134 701S–705SCrossRefGoogle ScholarPubMed
Moyer-Mileur, L, Chan, GM & Gill, G 1992 Evaluation of liquid and powered fortification of human milk on growth and bone mineralization status of preterm infants. Journal of Pediatric Gastroenterology & Nutrition 15 370–374Google Scholar
Mora-Gutierrez, A, Kumosinski, TF, & Farrell, HM Jr 1991 Quantification of αs1-casein in goat milk from French-Alpine and Anglo-Nubian breeds using reversed-phase HPLC. Journal of Dairy Science 74 3303–3307CrossRefGoogle Scholar
Mora-Gutierrez, A, Farrell, HM Jr & Kumosinski, TF 1993 Modeling calcium-induced solubility in caprine milk caseins using a thermodynamic linkage approach. Journal of Dairy Science 76 3698–3710CrossRefGoogle Scholar
Mora-Gutierrez, A & Farrell, HM Jr 2001 Hydration of native and cold-solubilized rennin-treated caprine caseins as determined by oxygen-17 nuclear magnetic resonance. Journal of Dairy Science 84 E93–E99CrossRefGoogle Scholar
Mora-Gutierrez, A, Farrell, HM Jr, Attaie, R, McWhinney, VJ & Wang, C 2007 Effects of bovine and caprine Monterey Jack cheeses fortified with milk calcium on bone mineralization in rats. International Dairy Journal 17 255–267CrossRefGoogle Scholar
Nickel, KP, Martin, BR, Smith, DL, Smith, JB, Miller, GD & Weaver, CM 1996 Calcium bioavailability from bovine milk and dairy products in premenopausal women using intrinsic and extrinsic labeling techniques. Journal of Nutrition 126 1406–1411CrossRefGoogle ScholarPubMed
Nicklas, TA 2003 Calcium intake trends and health consequences from childhood through adulthood. Journal of American College of Nutrition 22 340–356CrossRefGoogle ScholarPubMed
Remeuf, F, Verdalet-Guzman, I & Lenoir, J 1995 Technological adjustment of goat milk low synthesis-rate αs1-casein variants. International Dairy Journal 5 381–392CrossRefGoogle Scholar
Saito, Y, Sook, Y & Kimura, S 1998 Minimum effective dose of casein phosphopeptides (CPP) for enhancement of calcium absorption in growing rats. International Journal of Vitamins and Nutrition 68 335–340Google ScholarPubMed
Statistical Analysis Systems 1999 User Guide Statistics. SAS Institute, Inc., Cary, NC.Google Scholar
Smyth, M & FitzGerald, RJ 1997 Characterisation of a new chromatography matrix for peptide molecular mass determination. International Dairy Journal 7 571–577CrossRefGoogle Scholar
Talbot, JR, Guardo, P, Seccia, S, Gear, L, Lubary, DR, Saad, G, Roberts, ML, Fradinger, E, Marino, A & Zanchetta, JR 1999 Calcium bio availability and parathyroid hormone acute changes after oral intake of dairy and nondairy products in healthy volunteers. Osteoporosis International 10 137–142CrossRefGoogle Scholar
Teucher, B, Majsak-Newman, G, Dainty, JR, McDonagh, D, FitzGerald, RJ, & Fairweather-Tait, SJ 2006 Calcium absorption is not increased by caseinophosphopeptides. American Journal of Clinical Nutrition 84 162–166CrossRefGoogle Scholar
Tsuchita, H, Sekiguchi, I, Kuwata, T, Igarashi, C & Ezawa, I 1993 The effect of casein phosphopeptides on calcium utilization in young ovarectomized rats. Z Ernâhrungswis 32 121–124CrossRefGoogle Scholar
Tsuchita, H, Suzuki, T & Kuwata, T 2001 The effect of casein phosphopeptides on calcium absorption from calcium-fortified milk in growing rats. British Journal of Nutrition 85 5–10CrossRefGoogle ScholarPubMed
Tunick, MH 1987 Calcium in dairy products. Journal of Dairy Science 70 2429–2438CrossRefGoogle ScholarPubMed
Turnlund, JR 1991 Bioavailability of dietary minerals to humans: the stable isotope approach. Critical Reviews in Food Science and Nutrition 30 387–396CrossRefGoogle ScholarPubMed
Volek, JS 2003 Increasing fluid milk favourably affects bone mineral density responses to resistance training in adolescent boys-research and professional briefs. Journal of American Dietetics Association 103 1353–1356CrossRefGoogle Scholar
Weaver, CM 1992 Calcium bioavailability and its relation to osteoporosis. Proceedings of the Society for Experimental Biology and Medicine 200 157–160CrossRefGoogle Scholar
Zemel, MB & Miller, SL 2004 Dietary calcium and dairy modulation of adiposity and obesity risk. Nutrition Reviews 62 125–131CrossRefGoogle ScholarPubMed