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Inhibition of the proteinase activity in mastitic milk

Published online by Cambridge University Press:  01 June 2009

Weihuan Fang  and
Markus Sandholm
Weihuan Fang
Affiliation:
Department of Pharmacology and Toxicology, Department of Veterinary Medicine, Hämeentie 57, FIN-00580 Helsinki, Finland
Markus Sandholm
Affiliation:
Department of Pharmacology and Toxicology, Department of Veterinary Medicine, Hämeentie 57, FIN-00580 Helsinki, Finland
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Summary

The antiproteolytic activity of selected proteinase inhibitors was studied in mastitic bovine milk and urokinase-activated normal milk using a caseolytic agar diffusion assay. The inhibition profiles of mastitic milk and urokinase-activated milk were compared with those of purified proteinases. The proteinase inhibition profile of mastitic milk did not resemble that of any of the pure proteinases, indicating a mixed type of proteinase system in mastitic milk. The trypanocidals diminazene (equivalent to Berenil®) and pentamidine (equivalent to Lomidine®), together with aprotinin (Trasylol®), showed most promise when considering possible applications in mastitis to break up the proteolytic cascade within the inflamed udder.

Type
Original articles
Information
Journal of Dairy Research , Volume 62 , Issue 1 , February 1995 , pp. 61 - 68
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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