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Interaction between Lactobacillus kefir and Saccharomyces lipolytica isolated from kefir grains: evidence for lectin-like activity of bacterial surface proteins

Published online by Cambridge University Press:  05 January 2009

Marina Alejandra Golowczyc ,
Pablo Mobili ,
Graciela Liliana Garrote ,
María de los Angeles Serradell ,
Analía Graciela Abraham  and
Graciela Liliana De Antoni
Marina Alejandra Golowczyc
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA). 47 y 116 (1900). Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Consejo Nacional de Investigaciones Cientificas y Técnicas (CCT CONICET La Plata), Argentina.
Pablo Mobili
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA). 47 y 116 (1900). Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Consejo Nacional de Investigaciones Cientificas y Técnicas (CCT CONICET La Plata), Argentina.
Graciela Liliana Garrote
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA). 47 y 116 (1900). Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Cátedra de Microbiología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Consejo Nacional de Investigaciones Cientificas y Técnicas (CCT CONICET La Plata), Argentina.
María de los Angeles Serradell
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA). 47 y 116 (1900). Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Cátedra de Microbiología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
Analía Graciela Abraham*
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA). 47 y 116 (1900). Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Consejo Nacional de Investigaciones Cientificas y Técnicas (CCT CONICET La Plata), Argentina. Area Bioquímica y Control de Alimentos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
Graciela Liliana De Antoni
Affiliation:
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA). 47 y 116 (1900). Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Cátedra de Microbiología, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA), Argentina.
*
*For correspondence; e-mail: aga@biol.unlp.edu.ar
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Abstract

Several microbial interactions involving yeast and lactobacilli have been suggested in fermented products. Co-aggregation between Lactobacillus kefir and yeast Saccharomyces lipolytica isolated from kefir grains was studied by scanning electron microscopy and aggregation assays. Six out of twenty Lb. kefir strains were able to co-aggregate with Sacch. lipolytica CIDCA 812 and showed thermolabile non-covalently bound surface molecules involved in this interaction. Co-aggregation inhibition after Lb. kefir pre-treatment with 5 m-LiCl or 20 g SDS/l showed that bacterial S-layer proteins play an important role in this interaction. Presence of different sugar (mannose, sucrose and fructose) or yeast pre-treatment with sodium periodate inhibited co-aggregation between Lb. kefir and Sacch. lipolytica. Co-aggregating Lb. kefir strains were also able to agglutinate with human red blood cells and they lost this ability after treatment with 5 m-LiCl. These results and the capacity of purified S-layer proteins of Lb. kefir to haemagglutinate, strongly suggest that a lectin-like activity of bacterial surface proteins (S-layer) mediates the aggregation with yeast cells.

Keywords

kefir microorganismssurface proteinslectinsco-aggregation
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 1 , February 2009 , pp. 111 - 116
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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References

Angulo, L, Lopez, E & Lema, C 1993 Microflora present in kefir grains of the Galician Region (North-West of Spain). Journal of Dairy Research 60 263–267CrossRefGoogle ScholarPubMed
Antikainen, J, Anton, L, Sillanpaa, J & Korhonen, T 2002 Domains in the S-layer protein CbsA of Lactobacillus crispatus involved in adherence to collagens, laminin and lipoteichoic acids and self-assembly. Molecular Microbiology 2 381–394CrossRefGoogle Scholar
Avall-Jääskeläinen, S & Palva, A 2005 Lactobacillus surface layers and their applications. FEMS Microbiology Reviews 29 511–529CrossRefGoogle ScholarPubMed
Bibiloni, R, Perez, PF, Garrote, GL, Disalvo, EA & De Antoni, GL 2001 Surface characterization and adhesive properties of Bifidobacteria. In Methods in Enzymology, Vol. 336, pp. 411–427 (Ed. Doyle, RJ Microbial Growth in Biofilms. Part A. Developmental and Molecular Biological Aspects. Academic PressGoogle Scholar
Boyd, WC & Shapleigh, E 1954 Antigenic relations of blood group antigens as suggested by tests with lectins. Journal of Immunology 73 226–231CrossRefGoogle ScholarPubMed
Chaffin, WL, Lopez-Ribot, JL, Casanova, M, Gozalbo, D & Martinez, JP 1998 Cell wall and secreted proteins of Candida albicans: identification, function, and expression. Microbiology and Molecular Biology Reviews 62 30–180CrossRefGoogle ScholarPubMed
De Leeuw, E, Li, X & Lu, W 2006 Binding characteristics of the Lactobacillus brevis ATCC 8287 surface layer to extracellular matrix. FEMS Microbiology Letters 260 210–215Google ScholarPubMed
Engelhardt, H & Peters, J 1998 Structural research on surface layers: a focus on stability, surface layer homology domains, and surface layer-cell wall interactions. Journal of Structural Biology 124 276–302CrossRefGoogle ScholarPubMed
Farnwoth, ER 2005 Kefir – a complex probiotic. Food Science and Technology Bulletin 2 1–17Google Scholar
Garrote, G, Abraham, AG & De Antoni, G 2000 Inhibitory power of kefir: the role of organics acids. Journal of Food Protection 63 364–369CrossRefGoogle Scholar
Garrote, G, Abraham, A & De Antoni, GL 2001 Chemical and microbiological characterization of kefir grains. Journal of Dairy Research 68 639–652CrossRefGoogle ScholarPubMed
Garrote, G, Delfederico, L, Bibiloni, R, Abraham, A, Pérez, P, Semorile, L & De Antoni, G 2004 Lactobacilli isolated from kefir grains: evidence of the presence of S-layer proteins. Journal of Dairy Research 71 222–230CrossRefGoogle ScholarPubMed
Golowczyc, M, Mobili, P, Garrote, GL, Abraham, AG & De Antoni, GL 2007 Protective action of Lactobacillus kefir carrying S-layer protein against Salmonella enterica serovar Enteritidis. International Journal of Food Microbiology 118 264–273CrossRefGoogle ScholarPubMed
Jakava-Viljanen, M & Palva, A 2007 Isolation of surface (S) layer protein carrying Lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues. Veterinary Microbiology 124 264–273CrossRefGoogle Scholar
Kos, B, Šuškovic, J, Vukovic, S, Šimpraga, M, Frece, J & Matošic, S 2003 Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. Journal of Applied Microbiology 94 981–987CrossRefGoogle ScholarPubMed
Lakhtin, VM, Lakhtin, MV, Pospelova, VV & Shenderov, BA 2006 Lactobacilli and bifidobacteria lectins as possible signal molecules regulating intra- and interpopulation bacteria-bacteria and host-bacteria relationships. Part I. Methods of bacterial lectin isolation, physico-chemical characterization and some biological activity investigation. Microbial Ecology Health Disease 18 55–60CrossRefGoogle Scholar
Millsap, KW, Van Der Mei, HC, Bos, R & Busscher, HJ 1998 Adhesive interactions between medically important yeasts and bacteria. FEMS Microbiological Reviews 21 321–336CrossRefGoogle Scholar
Mukai, T, Kaneko, S & Ohori, H 1998 Haemagglutination and glycolipid-binding activities of Lactobacillus reuteri. Letters in Applied Microbiology 27 130–134CrossRefGoogle ScholarPubMed
Nikolaev, YA & Plakunov, VK 2007 Biofilms – “city of microbes” or an analogue of multicellular microorganims? Microbiology 76 125–138CrossRefGoogle ScholarPubMed
Ocaña, VS & Nader-Macías, ME 2002 Vaginal lactobacilli: self- and co-aggregating ability. British Journal of Biomedical Science 59 183–190CrossRefGoogle ScholarPubMed
Pretzer, G, Snel, J, Molenaar, D, Wiersma, A, Bron, PA, Lambert, J, de Vos, WM, van der Meer, R, Smits, MA & Kleerebezem, M 2005 Biodiversity-based identification and functional characterization of the mannose-specific adhesin of Lactobacillus plantarum. Journal of Bacteriology 187 6128–6136CrossRefGoogle ScholarPubMed
Roos, S & Jonsson, H 2002 A high molecular mass cell surface protein from Lactobacillus reuteri 1063 adheres to mucus components. Microbiology 148 433–442CrossRefGoogle ScholarPubMed
Sun, J, Le, G-W, Shi, Y-H & Su, G-W 2007 Factors involved in binding of Lactobacillus plantarum Lp6 to rat small intestinal mucus. Letters in Applied Microbiology 44 79–85CrossRefGoogle ScholarPubMed
Tielker, D, Hacker, S, Loris, R, Strathmann, M, Wingender, J, Wilhelm, S, Rosenau, F & Jaeger, KE 2005 Pseudomonas aeruginosa lectin LecB is located in the outer membrane and is involved in biofilm formation. Microbiology 151 1313–1323CrossRefGoogle ScholarPubMed
Uchida, H, Kinoshita, H, Kawai, Y, Kitazawa, H, Miura, K, Shiiba, K, Horii, A, Kimura, K, Taketomo, N, Oda, M, Yajima, T & Saito, T 2006a Lactic acid bacteria (LAB) bind to human B- or H-antigens expressed on intestinal mucosa. Bioscience Biotechnology and Biochemistry 70 3073–3076CrossRefGoogle ScholarPubMed
Uchida, H, Kinoshita, H, Kawai, Y, Kitazawa, H, Miura, K, Shiiba, K, Horii, A, Kimura, K, Taketomo, N, Oda, M, Yajima, T & Saito, T 2006b Lactobacilli binding human A-antigen expressed in intestinal mucosa. Research in Microbiology 157 659–665CrossRefGoogle ScholarPubMed