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Investigation of mammary blood flow changes by transrectal colour Doppler sonography in an Escherichia coli mastitis model

Published online by Cambridge University Press:  12 April 2010

André Potapow ,
Carola Sauter-Louis ,
Sandra Schmauder ,
Jutta Friker ,
Cordula Poulsen Nautrup ,
Daniel Mehne ,
Wolfram Petzl  and
Holm Zerbe
André Potapow*
Affiliation:
Clinic for Ruminants with Ambulance Clinic and Herd Health Management, Ludwig-Maximilians-University Munich, Germany
Carola Sauter-Louis
Affiliation:
Clinic for Ruminants with Ambulance Clinic and Herd Health Management, Ludwig-Maximilians-University Munich, Germany
Sandra Schmauder
Affiliation:
Clinic for Ruminants with Ambulance Clinic and Herd Health Management, Ludwig-Maximilians-University Munich, Germany
Jutta Friker
Affiliation:
Institute for Veterinary Anatomy, Ludwig-Maximilians-University Munich, Germany
Cordula Poulsen Nautrup
Affiliation:
Institute for Veterinary Anatomy, Ludwig-Maximilians-University Munich, Germany
Daniel Mehne
Affiliation:
Clinic for Ruminants with Ambulance Clinic and Herd Health Management, Ludwig-Maximilians-University Munich, Germany
Wolfram Petzl
Affiliation:
Clinic for Ruminants with Ambulance Clinic and Herd Health Management, Ludwig-Maximilians-University Munich, Germany
Holm Zerbe
Affiliation:
Clinic for Ruminants with Ambulance Clinic and Herd Health Management, Ludwig-Maximilians-University Munich, Germany
*
*For correspondence; e-mail: potapow@gmx.de
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Abstract

The objectives of this preliminary investigation were to evaluate the feasibility of transrectal colour Doppler sonography (TCDS) for determining blood flow of the pudendoepigastric trunk in cows with experimentally induced Escherichia coli Mastitis. Five primiparous Holstein dairy cows, 4–6 months after calving, were examined in two trials. All monitored udder quarters were initially clinically healthy, somatic cell count (SCC) was <50 000 cells/ml and bacteriologically negative. The blood flow of the left and the right pudendoepigastric trunk was described by the blood flow volume (BFV). In the methodological part of the study, the intra-observer precision of the method was evaluated. The coefficients of variation of the BFV were 7·1% for the left and 9·4% for the right pudendoepigastric trunk. The intraclass correlation coefficients of the BFV were 0·99 (P<0·001) for the left and 0·75 (P=0·004) for the right vessel. BFV did not differ significantly between the left and the right side nor between pre- and post-milking nor between oestrus and dioestrus. In the experimental part of the study, significant differences of increasing BFV between 0 and 12 h p.i. (post infectionem) (P=0·043) and decreasing BFV between 12 and 24 h p.i. (P=0·043) were discovered for the pudendoepigastric trunk of the infected right side. In the left-right (control-infection) comparison a significant increase of the right BFV was observed at 12 h p.i. (P=0·043). The difference of an increasing SCC correlated positively with the difference of an increasing BFV between 0 and 12 h p.i. (Spearman's ρ=1·00; P=0·043) for the right infected side. It was shown that TCDS is a reproducible technique for investigating pathological mammary blood flow changes at an early stage of acute mastitis.

Keywords

Doppler sonographymammary blood flowpudendoepigastric trunkEscherichia coli mastitiscow
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 2 , May 2010 , pp. 205 - 212
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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References

Bannermann, DD, Paape, MJ, Lee, JW, Zhao, X, Hope, JC & Rainard, P 2004 Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection. Clinical and Diagnostic Laboratory Immunology 11 463–472Google Scholar
Bollwein, H, Maierl, J, Mayer, R & Stolla, R 1998 Transrectal color Doppler sonography of the A. uterina in cyclic mares. Theriogenology 49 1483–1488Google Scholar
Bollwein, H, Meyer, HHD, Maierl, J, Weber, F, Baumgartner, U & Stolla, R 2000 Transrectal Doppler sonography of uterine blood flow in cows during the estrous cycle. Theriogenology 53 1541–1552Google Scholar
Bollwein, H, Baumgartner, U & Stolla, R 2002 Transrectal Doppler sonography of uterine blood flow in cows during pregnancy. Theriogenology 57 2053–2061CrossRefGoogle ScholarPubMed
Bollwein, H, Weber, F, Woschée, I & Stolla, R 2004 Transrectal Doppler sonography of uterine and umbilical blood flow during pregnancy in mares. Theriogenology 6 499–509CrossRefGoogle Scholar
Bortz, J 1993 [Chapter 5: Verification methods of difference hypotheses]. In: Statistik für Sozialwissenschaftler 4th Edn, pp. 144–145Berlin, Germany: Springer VerlagGoogle Scholar
Budras, KD, Wünsche, A 2002 [Arteries, veins and nerves of the pelvic cavity]. In: Atlas der Anatomie des Rindes: Lehrbuch für Tierärzte und Studierende. Schlütersche, 1st Edn (Eds Budras, KD & Wünsche, A) pp. 84–87. Hannover, GermanyGoogle Scholar
Burvenich, C 1980 Variations of mammary artery blood flow and milk yield under normal conditions and during the oestrus cycle of the dairy goat. Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 43 18–27CrossRefGoogle ScholarPubMed
Burvenich, C, Paape, MJ, Hoeben, D, Dosogne, H, Massart-Leen, AM & Blum, J 1999 Modulation of the inflammatory reaction and neutrophil defence of the bovine lactating mammary gland by growth hormone. Domestic Animal Endocrinology 17 149–159Google Scholar
Christensen, K, Nielsen, MO, Bauer, R & Hilden, K 1989 Evaluation of mammary blood flow measurements in lactating goats using the ultrasound Doppler principle. Comparative Biochemistry and Physiology 92A 385–392CrossRefGoogle Scholar
Davis, SR & Collier, RJ 1985 Mammary blood flow and regulation of substrate supply for milk synthesis. Journal of Dairy Science 68 1041–1058Google Scholar
De Mol, RM & Ouweltjes, W 2001 Detection model for mastitis in cows milked in an automatic milking system. Preventive Veterinary Medicine 49 71–82CrossRefGoogle Scholar
Dhondt, G, Burvenich, C & Peeters, G 1977 Mammary blood flow during experimental Escherichia coli endotoxin induced mastitis in goats and cows. Journal of Dairy Research 44 433–440CrossRefGoogle ScholarPubMed
Dosogne, H, Burvenich, C, van Weren, T, Roets, E, Noordhuizen-Stassen, EN & Goddeeris, B 1997 Increased surface expression of CD11b receptors on polymorphonuclear leucocytes is not sufficient to sustain phagocytosis during Escherichia coli mastitis in early postpartum dairy cows. Veterinary Immunology and Immunopathology 60 47–59CrossRefGoogle Scholar
Grunert, E (1990): [Udder]. In: Die klinische Untersuchung des Rindes 3rd Edn. (Ed. Rosenberger, G) p. 525. Berlin and Hamburg, Germany: Verlag Paul PareyGoogle Scholar
Hollis, B, Mavrides, E, Campbell, S, Tekay, A & Thilaganathan, B 2001 Reproducibility and repeatability of transabdominal uterine artery Doppler velocimetry between 10 and 14 weeks of gestation. Ultrasound in Obstetrics and Gynecology 18 593–597CrossRefGoogle ScholarPubMed
Houvenaghel, A, Peters, G & Verschooten, F 1973 Influences of manual udder stimulation and oxytocin on mammary blood flow in lactating cows. Archives Internationales de Pharmacodynamie et de Thérapie 205 124Google ScholarPubMed
Krueger, L, Koerte, J, Tsousis, G, Herzog, K, Flachowsky, G & Bollwein, H 2009 Transrectal Doppler sonography of uterine blood flow during the first 12 weeks after parturition in healthy dairy cows. Animal Reproduction Science 114 23–31CrossRefGoogle ScholarPubMed
Lamb, CR, Burton, CA & Carlisle, CH 1999 Doppler measurements of hepatic arterial flow in dogs: technique and preliminary findings. Veterinary Radiology and Ultrasound 40 77–81CrossRefGoogle ScholarPubMed
Lough, DS, Beede, DL & Wilcox, CJ 1990 Effects of feed intake and thermal stress on mammary blood flow and other physiological measurements in lactating dairy cows. Journal of Dairy Science 73 325–332CrossRefGoogle ScholarPubMed
Meurer, DG 1999 [Stages of the inflammation]. In: Allgemeine Pathologie: Kompendium für die Veterinärmedizin pp. 118–119. Schattauer Stuttgart, Germany: Verlagsgesellschaft mbHGoogle Scholar
Miller, GY, Bartlett, PC, Lance, SE, Anderson, J & Heider, LE 1993 Costs of clinical mastitis and mastitis prevention in dairy herds. Journal of the American Veterinary Medical Association A 202 1230–1236CrossRefGoogle ScholarPubMed
Petzl, W, Zerbe, H, Günther, J, Yang, W, Seyfert, H-M, Nürnberg, G & Schuberth, H-J 2008 Escherichia coli, but not Staphylococcus aureus triggers an early increased expression of factors contributing to the innate immune defense in the udder of the cow. Veterinary Research 39 18Google Scholar
Piccione, G, Arcigli, A, Fazio, F, Giudice, E & Caola, G 2004a Pulsed wave-doppler ultrasonographic evaluation of mammary blood flow speed in cows during different productive periods. Acta Scientiae Veterinariae 32 171–175CrossRefGoogle Scholar
Piccione, G, Arcigli, A, Assenza, A, Percipalle, M & Caola, G 2004b Pulsed wave-Doppler ultrasonographic evaluation of the mammary blood flow in the ewe. Acta Veterinaria 73 23–27CrossRefGoogle Scholar
Prakash, BS, Meyer, HHD, Schallenberger, E & van de Wiel, DFM 1987 Development of a sensitive enzymeimmunoassay (EIA) for progesterone determination in unextracted bovine plasma using the antibody technique. Journal of Steroid Biochemistry 28 623–627CrossRefGoogle ScholarPubMed
Rajala-Schultz, PJ & Gröhn, YT 1999 Culling of dairy cows. Part I. Effects of diseases on culling in Finnish Ayrshire cows. Preventive Veterinary Medicine 41 195–208Google Scholar
Rauch, A, Krueger, L, Miyamoto, A & Bollwein, H 2008 Color Doppler sonography of cystic ovarian follicles in cows. Journal of Reproduction and Development 54 447–453Google Scholar
Schmucker, N, Schatzmann, U, Budde, K, Gundel, M, Jäggin, CE & Meier, H 2000 Duplex-ultrasonographic evaluation of the common carotid artery in the resting, sedated and anesthetized horse. Veterinary Radiology and Ultrasound 41 168–171CrossRefGoogle ScholarPubMed
Seegers, H, Fourichon, C & Beaudeau, F 2003 Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research 34 475–491Google Scholar
Vanselow, J, Yang, W, Herrmann, J, Zerbe, H, Schuberth, HJ, Petzl, W, Tomek, W & Seyfert, HM 2006 DNA-remethylation around a STAT5-binding enhancer in the alphaS1-casein promoter is associated with abrupt shutdown of alpha-s1-casein synthesis during acute mastitis. Journal of Molecular Endocrinology 37 463–477Google Scholar