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Isolation and characterisation of lactic acid bacteria from donkey milk

Published online by Cambridge University Press:  07 September 2016

Maria de los Dolores Soto del Rio ,
Christian Andrighetto ,
Alessandra Dalmasso ,
Angiolella Lombardi ,
Tiziana Civera  and
Maria Teresa Bottero
Maria de los Dolores Soto del Rio
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Christian Andrighetto
Affiliation:
Veneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, Via San Gaetano 74, 36016 Thiene (VI), Italy
Alessandra Dalmasso
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Angiolella Lombardi
Affiliation:
Veneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, Via San Gaetano 74, 36016 Thiene (VI), Italy
Tiziana Civera*
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Maria Teresa Bottero
Affiliation:
Dipartimento di Scienze Veterinarie, Università di Torino, Largo Braccini 2, 10095 Grugliasco (TO), Italy
*
*For correspondence; e-mail: tiziana.civera@unito.it
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Abstract

During the last years the interest in donkey milk has increased significantly mainly because of its compelling functional elements. Even if the composition and nutritional properties of donkey milk are known, its microbiota is less studied. This Research Communication aimed to provide a comprehensive characterisation of the lactic acid bacteria in raw donkey milk. RAPD-PCR assay combined with 16S rDNA sequencing analysis were used to describe the microbial diversity of several donkey farms in the North West part of Italy. The more frequently detected species were: Lactobacillus paracasei, Lactococcus lactis and Carnobacterium maltaromaticum. Less abundant genera were Leuconostoc, Enterococcus and Streptococcus. The yeast Kluyveromyces marxianus was also isolated. The bacterial and biotype distribution notably diverged among the farms. Several of the found species, not previously detected in donkey milk, could have an important probiotic activity and biotechnological potential. This study represents an important insight to the ample diversity of the microorganisms present in the highly selective ecosystem of raw donkey milk.

Keywords

Donkey milklactic acid bacteriaRAPD-PCR characterisation
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 383 - 386
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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References

Afzal, MI, Jacquet, T, Delaunay, S, Borges, F, Millière, JB, Revol-Junelles, AM & Cailliez-Grimal, C 2010 Carnobacterium maltaromaticum: identification, isolation tools, ecology and technological aspects in dairy products. Food Microbiology 27 573–579 Google Scholar
Andrighetto, C, Borney, F, Barmaz, A, Stefanon, B & Lombardi, A 2002 Genetic diversity of Streptococcus thermophilus strains isolated from Italian traditional cheeses. International Dairy Journal 12 141–144 Google Scholar
Ashokkumar, S, Sree Krishna, R, Pavithra, V, Hemalatha, V & Priya, I 2011 Production and antibacterial activity of bacteriocin by Lactobacillus paracasei isolated from donkey milk. International Journal of Current Science 1 109–115 Google Scholar
Carminati, D, Tidona, F, Fornasari, ME, Rossetti, L, Meucci, A & Giraffa, G 2014 Biotyping of cultivable lactic acid bacteria isolated from donkey milk. Letters in Applied Microbiology 59 299–305 Google Scholar
Cavallarin, L, Giribaldi, M, Soto-Del Rio, MD, Valle, E, Barbarino, G, Gennero, MS & Civera, T 2015 A survey on the milk chemical and microbiological quality in dairy donkey farms located in NorthWestern Italy. Food Control 50 230–235 Google Scholar
Chiavari, C, Coloretti, F, Nanni, M, Sorrentino, E & Grazia, L 2005 Use of donkey's milk for a fermented beverage with lactobacilli. Lait 85 481–490 Google Scholar
Maccaferri, S, Klinder, A, Brigidi, P, Cavina, P & Costabile, A 2012 Potential probiotic Kluyveromyces marxianus B0399 modulates the immune response in Caco-2 cells and peripheral blood mononuclear cells and impacts the human gut microbiota in an in vitro colonic model system. Applied and Environmental Microbiology 78 956–964 Google Scholar
Mansueto, P, Iacono, G, Taormina, G, Seidita, A, D'Alcamo, A, Adragna, F, Randazzo, G, Carta, M, Rini, G & Carroccio, A 2013 Ass's milk in allergy to cow's milk protein: a review. Acta Medica Mediterranea 29 153–160 Google Scholar
Perna, A, Intaglietta, I, Simonetti, A & Gambacorta, E 2015 Donkey milk for manufacture of novel functional fermented beverages. Journal of Food Science 80 S1352–S1359 Google Scholar
Quigley, L, O'Sullivan, O, Stanton, C, Beresford, TP, Ross, RP, Fitzgerald, GF & Cotter, PD 2013 The complex microbiota of raw milk. FEMS Microbiology Reviews 37 664–698 Google Scholar
Ruaro, A, Andrighetto, C, Torriani, S & Lombardi, A 2013 Biodiversity and characterization of indigenous coagulase-negative staphylococci isolated from raw milk and cheese of North Italy. Food Microbiology 34 106–111 CrossRefGoogle ScholarPubMed
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