Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-28T20:34:23.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Ketotic cows display a different serum nonesterified fatty acid composition

Published online by Cambridge University Press:  31 January 2020

Lei Liu ,
Taiyu Shen ,
Wei Yang ,
Hongjiang Yu ,
Sansi Gao ,
Baoyin Huang  and
Chuang Xu
Lei Liu
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China College of Veterinary Medicine, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Collaborative innovation center of safety production of livestock and poultry in Hunan, Hunan Agricultural University, Changsha, Hunan, 410128, China
Taiyu Shen
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China
Wei Yang
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China
Hongjiang Yu
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China
Sansi Gao
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China
Baoyin Huang
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China
Chuang Xu*
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China
*
Author for correspondence: Chuang Xu, Email: xuchuang7175@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

The experiments reported in this research communication aimed to compare the serum nonesterified fatty acid (NEFA) composition in ketotic cows and healthy cows during the perinatal period. NEFAs play significant roles in etiology and pathology of ketosis. We hypothesized that ketotic cows will display a different serum NEFA composition compared to healthy controls, and fatty acid related indicators for ketosis prediction can be screened. Pre-partum healthy cows were recruited, and blood samples were collected on −7, 3, 7, 14 and 21 d postpartum. Cows were further divided into a healthy control group (C group, n = 6) and a ketosis group (K group, n = 6) if blood β-hydroxybutyric acid levels exceeded 1.2 mm during the experiment. NEFA composition was then analyzed by means of Gas Chromatography-Mass Spectrometer (GC-MS). Only C12 : 0% was significantly higher in C group than K group on 7 d pre-partum (P < 0.05), when the cows were not diagnosed with ketosis. Five fatty acids displayed statistical differences in composition between C and K group (P < 0.05), namely C12 : 0, C16 : 0, C17 : 0, C18 : 1n9 and C22 : 1n9. Saturates%, unsaturates%, mono-unsaturates% and saturates/unsaturates were also different between C and K group (P < 0.05). Of note, C18 : 1n9/C12 : 0 and C18 : 1n9/C22 : 1n9 in K group were significantly higher than those in controls on 7 d pre-partum (P < 0.05). It is suggested that the ratios show potential as indicators for prediction of ketosis.

Keywords

de novo lipogenesisdisease predictingketosisNEFA
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 1 , February 2020 , pp. 52 - 55
Copyright
Copyright © Hannah Dairy Research Foundation 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Butterfield, R (1966) The effect of nutritional stress and recovery on the body composition of cattle. Research in Veterinary Science 7, 168–179.Google ScholarPubMed
Contreras, GA, O'boyle, NJ, Herdt, TH and Sordillo, LM (2010) Lipomobilization in periparturient dairy cows influences the composition of plasma nonesterified fatty acids and leukocyte phospholipid fatty acids. Journal of Dairy Science 93, 2508–2516.Google ScholarPubMed
Nielsen, NI, Friggens, NC, Chagunda, MGG and Ingvartsen, KL (2005) Predicting risk of ketosis in dairy cows using in-line measurements of β-hydroxybutyrate: a biological model. Journal of Dairy Science 88, 2441–2453.10.3168/jds.S0022-0302(05)72922-2Google ScholarPubMed
Rukkwamsuk, T, Geelen, MJH, Kruip, TAM and Wensing, T (2000) Interrelation of fatty acid composition in adipose tissue, serum, and liver of dairy cows during the development of fatty liver postpartum. Journal of Dairy Science 83, 52–59.10.3168/jds.S0022-0302(00)74854-5Google ScholarPubMed
Vernon, RG (1980) Lipid metabolism in the adipose tissue of ruminant animals. Progress in Lipid Research 19, 23–106.Google ScholarPubMed
Wang, J, Zhu, X, She, G, Kong, Y, Guo, Y, Wang, Z, Liu, G and Zhao, B (2018) Serum hepatokines in dairy cows: periparturient variation and changes in energy-related metabolic disorders. BMC Veterinary Research 14, 236.Google ScholarPubMed
Xu, Z, Harvey, K, Pavlina, T, Dutot, G, Zaloga, G and Siddiqui, R (2010) An improved method for determining medium- and long-chain FAMEs using gas chromatography. Lipids 45, 199–208.10.1007/s11745-009-3382-7Google ScholarPubMed
Yamdagni, S and Schultz, L (1970) Fatty acid composition of blood plasma lipids of normal and ketotic cows. Journal of Dairy Science 53, 1046–1050.10.3168/jds.S0022-0302(70)86343-3Google ScholarPubMed
Zhang, B, Guo, H, Yang, W, Li, M, Zou, Y, Loor, JJ, Xia, C and Xu, C (2019) Effects of ORAI calcium release-activated calcium modulator 1 (ORAI1) on neutrophil activity in dairy cows with subclinical hypocalcemia. Journal of Animal Science 97, 3326–3336.10.1093/jas/skz209Google Scholar
Supplementary material: PDF

Liu et al. supplementary material

Liu et al. supplementary material

Download Liu et al. supplementary material(PDF)
PDF 281.5 KB