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Leucocyte expression of genes implicated in the plasminogen activation cascade is modulated by yoghurt peptides

Published online by Cambridge University Press:  07 September 2016

Georgios Theodorou  and
Ioannis Politis
Georgios Theodorou*
Affiliation:
Department of Animal Science and Aquaculture, Agricultural University of Athens, Athens, Greece
Ioannis Politis
Affiliation:
Department of Animal Science and Aquaculture, Agricultural University of Athens, Athens, Greece
*
*For correspondence; e-mail: gtheod@aua.gr
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Abstract

The urokinase-plasminogen activator (u-PA), its receptor (u-PAR) and the inhibitors of u-PA (PAI-1 and PAI-2) provide a multi-molecular system in leucocytes that exerts pleiotropic functions influencing the development of inflammatory and immune responses. The objective of the present study was to examine the ability of water soluble extracts (WSE) obtained from traditional Greek yoghurt made from bovine or ovine milk to modulate the expression of u-PA, u-PAR, PAI-1 and PAI-2 in ovine monocytes and neutrophils. WSE were obtained from 8 commercial traditional type Greek yoghurts made from ovine or bovine milk. WSE upregulated the expression of all 4 u-PA related genes in monocytes but the upregulation was much higher in the PAI-1 (10-fold) than in u-PA and u-PAR (3–4 fold) thus, shifting the system towards inhibition. In line with this observation, WSE reduced total and membrane-bound u-PA activity in monocytes. In neutrophils, WSE caused small (50–60%) but significant (P < 0·05) reductions in expression of u-PAR and PAI-2 but had no effect on expression of u-PA, PAI-1 and on total cell-associated and membrane-bound u-PA activity. WSE from yoghurts made from bovine or ovine milk were essentially equally effective in affecting the u-PA system except for the u-PAR gene in ovine neutrophils that was affected (reduced) by the ovine and not the bovine WSE. In conclusion, peptides present in WSE modulated the expression of u-PA related genes but the effect was much more prominent in monocytes than in neutrophils.

Keywords

Yoghurtplasminogen activation systemneutrophilsmonocytes
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 360 - 365
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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