Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-10T09:25:21.557Z Has data issue: false hasContentIssue false
  • English
  • Français

Microbiological and physicochemical characteristics of buffalo milk used for dairy products in southern Brazil

Published online by Cambridge University Press:  30 October 2020

Fernanda M. S. Godinho ,
Melina Krug ,
Renata Perin Figueiredo ,
Alexandre Müller ,
Louise Jank ,
Caroline Andrade Tomaszewski ,
Daniel Rodrigo Hillesheim ,
Éder J. Kinast ,
Ana Paula G. Frazzon  and
Amanda S. Motta
Fernanda M. S. Godinho
Affiliation:
Postgraduate Program in Agricultural and Environmental Microbiology (PPGMAA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil Centro de Desenvolvimento Científico e Tecnológico (CDCT), Centro Estadual de Vigilância em Saúde (CEVS), Porto Alegre, RS, Brazil
Melina Krug
Affiliation:
Postgraduate Program in Agricultural and Environmental Microbiology (PPGMAA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
Renata Perin Figueiredo
Affiliation:
Postgraduate Program in Agricultural and Environmental Microbiology (PPGMAA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
Alexandre Müller
Affiliation:
Postgraduate Program in Chemistry (PPGQ), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
Louise Jank
Affiliation:
Laboratório Federal de Defesa Agropecuária LFDA/RS, Ministério da Agricultura, Pecuária e Abastecimento, Porto Alegre, RS, Brazil
Caroline Andrade Tomaszewski
Affiliation:
Laboratório Federal de Defesa Agropecuária LFDA/RS, Ministério da Agricultura, Pecuária e Abastecimento, Porto Alegre, RS, Brazil
Daniel Rodrigo Hillesheim
Affiliation:
Laboratório Federal de Defesa Agropecuária LFDA/RS, Ministério da Agricultura, Pecuária e Abastecimento, Porto Alegre, RS, Brazil
Éder J. Kinast
Affiliation:
Universidade Estadual do Rio Grande do Sul (UERGS), Unidade Porto Alegre, Porto Alegre, RS, Brazil
Ana Paula G. Frazzon
Affiliation:
Postgraduate Program in Agricultural and Environmental Microbiology (PPGMAA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
Amanda S. Motta*
Affiliation:
Postgraduate Program in Agricultural and Environmental Microbiology (PPGMAA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
*
Author for correspondence: Amanda S. Motta, Email: amanda.motta@ufrgs.br
Get access Rights & Permissions [Opens in a new window]

Abstract

In Brazil, the buffalo milk market has been growing. However, identity and quality standards have not been established for this raw material, nor have proper distinctions between buffalo milk and bovine milk been defined. Currently, the State of Rio Grande do Sul (RS) has only three producers that supply raw material for officially marketed derivatives. The aim of this study was to determine the identity and quality standards of raw buffalo milk in this region. Samples were obtained biweekly from three farm cooling tanks between June 2017 and August 2018, to reach a total of 69 samples. The averages for the results of the physicochemical parameters fat, protein, lactose, total solids, SNF (solids-not-fat), calcium, density, FP, acidity and SCC were 5.5 g/100 g, 4.06 g/100 g, 5.07 g/100 g, 15.5 g/100 g, 9.96 g/100 g, 0.161 g/100 g, 1.034 g/ml, −0.527°C, 16°D and 95 × 103 cells/ml, respectively. With reference to the microbiological parameters, the mean of the Standard Plate Count (SPC) and thermotolerant coliforms were 9,0 × 104 CFU/ml and 1.6 × 102 MPN/ml, respectively. Regarding coagulase-positive staphylococci, 36 samples tested positive (52% of total). Neither Salmonella spp. nor Listeria monocytogenes, nor antibiotic or antiparasitic residues were detected in any sample. In conclusion, the buffalo milk used as raw material for dairy products in southern Brazil demonstrated satisfactory physicochemical and microbiological characteristics, in accordance with recent scientific literature.

Keywords

buffalo milkmicrobiologymilk propertiesmilk qualitylegislation
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 4 , November 2020 , pp. 463 - 468
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmad, S, Anjum, FM, Huma, N, Sameen, A and Zahoor, T (2013) Composition and physico-chemical characteristics of buffalo milk with particular emphasis on lipids, proteins, minerals, enzymes and vitamins. Journal of Animal and Plant Sciences 23, 62–74.Google Scholar
AOAC 991.25 (2002) Ca, Mg and P in cheese. In Latimer GW (Ed) Official Methods of Analysis of AOAC International, 20th Edn. vol II, AOAC International, Washington.Google Scholar
Araújo, TPM, Rangel, AHN, Soares, AD, Lima, TCC, Lima Júnior, DM and Novaes, LP (2011) Influence seasons of the year on milk composition buffaloes kept in farm milk tank. Agropecuária Científica no Semiárido 7, 1–5.Google Scholar
Ariota, B, Campanile, G, Potena, A, Napolano, R, Gasparrini, B, Neglia, GL and Di Palo, R (2007) Ca and P in buffalo milk: curd yield and milk clotting parameters. Italian Journal of Animal Science 6, 497–499.CrossRefGoogle Scholar
Bailone, RL, Borra, RC, Roça, RO, Aguiar, L and Harris, M (2017) Quality of refrigerated raw milk from buffalo cows (Bubalus bubalis bubalis) in different farms and seasons in Brazil. Ciência Animal Brasileira 18, 1–12.CrossRefGoogle Scholar
Barreto, F, Ribeiro, C, Hoff, RB and Costa, TD (2016) Determination of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in poultry, swine, bovine and fish by liquid chromatography–tandem mass spectrometry. Journal of Chromatography A 1449, 48–53.CrossRefGoogle ScholarPubMed
Bassbasi, M, Platikanov, S, Tauler, R and Oussama, A (2014) FTIR-ATR determination of solid non fat (SNF) in raw milk using PLS and SVM chemometric methods. Food Chemistry 146, 250–254.CrossRefGoogle ScholarPubMed
Brazil (2007) Human milk bank: operation, prevention and control of risks, Agência Nacional de Vigilância Sanitária – Ministério da Saúde, Brasília.Google Scholar
Brillinger, D (1984) The Collected Works of John W. Tukey, 1ª Edn. vol. II, Monterey, CA: SpringerGoogle Scholar
Chye, FY, Abdullah, A and Ayob, MK (2004) Bacteriological quality and safety of raw milk in Malaysia. Food Microbiology 21, 535–541.CrossRefGoogle Scholar
El-Salam, MHA and El-Shibiny, S (2011) A comprehensive review on the composition and properties of buffalo milk. Dairy Science & Technology 91, 663–699.CrossRefGoogle Scholar
Elshaghabee, FMF, Abdel-Hamid, MI and Walte, HG (2017) A survey on selected quality parameters of buffalo milk samples collected from consumer markets of three different central governorates in Egypt. Milk Science International 70, 25–29.Google Scholar
Figueiredo, EL, Junior, JBL and Toro, MJU (2010) Physical-chemical and microbiological characterization of buffalo milk ‘in natura’ produced in the state of Pará. Revista Brasileira de Tecnologia Agroindustrial 4, 19–28.Google Scholar
Filho, MHBC, Júnior, DML, Rangel, AHN, Silva, FJS, Novaes, LP, Júnior, JGBG, Silva, MJMS and Moreno, GMB (2014) Season and buffalo milk quality in Rio Grande do Norte state. Acta Veterinaria Brasílica 8, 201–208.Google Scholar
Gargouri, A, Hamed, H and Elfeki, A (2013) Analysis of raw milk quality at reception and during cold storage: combined effects of somatic cell counts and psychrotrophic bacteria on lipolysis. Journal of Food Science 78, M1405–M1411.CrossRefGoogle ScholarPubMed
Han, X, Lee, FL, Zhang, L and Guo, MR (2012) Chemical composition of water buffalo milk and its low-fat symbiotic yogurt development. Functional Foods in Health and Disease 2, 86–106.CrossRefGoogle Scholar
ISO 6888-1 (1999) Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – Part 1: Technique using Baird-Parker agar medium. International Organization for Standardization, Geneva.Google Scholar
ISO 7251 (2005) Microbiology of food and animal feeding stuffs – Horizontal method for the detection and enumeration of presumptive Escherichia coli – Most probable number technique. International Organization for Standardization, Geneva.Google Scholar
ISO 13366-2 (2006) Milk – Enumeration of somatic cells – Part 2: Guidance on the operation of fluoro-opto-electronic counters. International Organization for Standardization, Geneva.Google Scholar
ISO 4833-1 (2013) Microbiology of the food chain – Horizontal method for the enumeration of microorganisms – Part 1: Colony count at 30 degrees C by the pour plate technique. International Organization for Standardization, Geneva.Google Scholar
ISO 9622 (2013) Milk and liquid milk products – Guidelines for the application of mid-infrared spectrometry. International Organization for Standardization, Geneva.Google Scholar
ISO 11290-1 (2017) Microbiology of the food chain – Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Part 1: Detection method. International Organization for Standardization, Geneva.Google Scholar
ISO 6579-1 (2017) Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp. International Organization for Standardization, Geneva.Google Scholar
Jank, L, Martins, MT, Arsand, JB, Motta, TMC, Hoff, RB, Barreto, F and Pizzolato, TM (2015) High-throughput method for macrolides and lincosamides antibiotics residues analysis in milk and muscle using a simple liquid–liquid extraction technique and liquid chromatography–electrospray–tandem mass spectrometry analysis (LC–MS/MS). Talanta 144, 686–695.CrossRefGoogle Scholar
Khaniki, J (2007) Chemical contaminants in milk and public health concerns: a review. International Journal of Dairy Science 2, 104–115.Google Scholar
Khedkar, CD, Kalyankar, SD and Deosarkar, SS (2016) Buffalo milk. Encyclopedia of Food and health 1, 522–528.CrossRefGoogle Scholar
Pasquini, M, Osimani, A, Tavoletti, S, Moreno, I, Clementi, F and Trobetta, MF (2018) Trends in the quality and hygiene parameters of bulk Italian Mediterranean buffalo (Bubalus bubalis) milk: A three year study. Animal Science Journal 89, 176–185.CrossRefGoogle ScholarPubMed
Pesce, A, Salzano, C, De Felice, A, Garofalo, F, Liguori, S, De Santo, A, Palermo, P and Guarino, A (2016) Monitoring the freezing point of buffalo milk. Italian Journal of Food Safety 5, 95–97.CrossRefGoogle ScholarPubMed
Ricci, GD and Domingues, PF (2012) The buffalo milk. Journal of Continuing Education in Animal Science of CRMV-SP 10, 14–19.Google Scholar
Rübensam, G, Barreto, F, Hoff, RB, Kist, TL and Pizzolato, TM (2011) A liquid-liquid extraction procedure followed by a low temperature purification step for the analysis of macrocyclic lactones in milk by liquid chromatography-tandem mass spectrometry and fluorescence detection. Analytica Chimica Acta 705, 24–29.CrossRefGoogle ScholarPubMed
Sales, DC, Rangel, AHN, Urbano, SA, Tonhati, H, Galvão Júnior, JGB, Guilhermino, MM, Aguiar, EM and Bezerra, MF (2018) Buffalo milk composition, processing factors, whey constituents recovery and yield in manufacturing Mozzarella cheese. Food Science and Technology 38, 328–334.CrossRefGoogle Scholar
São Paulo (1994) SAA Resolution No. 24 of August 1, 1994: Lays down the technical standards for the production and classification of products of animal origin and those relating to the inspection and inspection of products of animal origin, Secretaria de Agricultura e Abastecimento, São Paulo.Google Scholar
Schukken, YH, Wilson, DJ, Welcome, F, Garrison-Tikofsky, L and Gonzalez, RN (2003) Monitoring udder health and milk quality using somatic cell counts. Veterinary Research 34, 579–596.CrossRefGoogle ScholarPubMed
Siegel, S and Castellan, NJJ (1988) Nonparametric Statistics for the Behavioral Sciences, 2ª Edn. New York, NY: Mcgraw-Hill Book Company.Google Scholar
Teixeira, LV, Bastianetto, E and Oliveira, DAA (2005) The water buffalo milk in milky industry. Revista Brasileira de Reprodução Animal 29, 96–100.Google Scholar
Tripaldi, C, Palocci, G, Miarelli, M, Catta, M, Orlandini, S, Amatiste, S, Bernardini, R and Castillo, G (2010) Effects of mastitis on buffalo milk quality. Asian-Australasian Journal of Animal Sciences 23, 1319–1324.CrossRefGoogle Scholar
Supplementary material: PDF

Godinho et al. supplementary material

Godinho et al. supplementary material

Download Godinho et al. supplementary material(PDF)
PDF 107.1 KB