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Molecular characteristics of bap-positive Staphylococcus aureus strains from dairy cow mastitis

Published online by Cambridge University Press:  08 April 2015

Gustavo GM Snel ,
Stefan Monecke ,
Ralf Ehricht  and
Renata Piccinini
Gustavo GM Snel
Affiliation:
Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy
Stefan Monecke
Affiliation:
Alere Technologies GmbH, Jena, Germany Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany
Ralf Ehricht
Affiliation:
Alere Technologies GmbH, Jena, Germany
Renata Piccinini*
Affiliation:
Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy
*
*For correspondence; e-mail: renata.piccinini@unimi.it
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Abstract

The biofilm-associated protein (Bap) of Staphylococcus aureus is a high molecular weight cell-wall-anchored protein involved in biofilm formation, first described in bovine mastitis strains from Spain. So far, studies regarding Bap were mainly based on the Spanish strain V329 and its mutants, but no information on the genetic variability of bap-positive Staph. aureus strains is yet available in the literature. The present study investigated the molecular characteristics of 8 bap-positive Staph. aureus strains from subclinical bovine mastitis, isolated in 5 herds; somatic cell counts (SCC) of milk samples were also registered. Strains were characterised using MLST, SPA typing and microarray and the results were compared with V329. All isolates from this study and V329 were assigned to ST126, t605, but some molecular differences were observed. Only herd A and B strains harboured the genes for β-lactams resistance; the leukocidin D/E gene, a type I site-specific deoxyribonuclease subunit, 3rd locus gene and serin-protease A and B were carried by all strains, but not by V329, while serin-protease E was absent in V329 and in another isolate. Four isolates and V329 harboured the fibronectin-binding protein B gene. SCC showed the highest value in the milk sample affected by the only strain carrying all the virulence factors considered. Potential large variability of virulence was evidenced among V329 and all bap-positive Staph. aureus strains considered: the carriage of fnb could enhance the accumulation of biofilm, but the lack of lukD/E and splA, B or E might decrease the invasiveness of strain.

Keywords

Staphylococcus aureusbap genebiofilmdairy cow mastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 3 , August 2015 , pp. 312 - 316
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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