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Monospecificity of the antibodies to bovine αs1-casein fragment 140–149: application to the detection of bovine milk in caprine dairy products

Published online by Cambridge University Press:  01 June 2009

Marie-Paule Rolland ,
Lotfi Bitri  and
Pierre Besançon
Marie-Paule Rolland
Affiliation:
Unité de Nutrition, Génie Biologique et Sciences des Aliments, Département Agroressources et Procédés Biologiques, Université de Montpellier II, 34095 Montpellier Cedex 05, France
Lotfi Bitri
Affiliation:
Unité de Nutrition, Génie Biologique et Sciences des Aliments, Département Agroressources et Procédés Biologiques, Université de Montpellier II, 34095 Montpellier Cedex 05, France
Pierre Besançon
Affiliation:
Unité de Nutrition, Génie Biologique et Sciences des Aliments, Département Agroressources et Procédés Biologiques, Université de Montpellier II, 34095 Montpellier Cedex 05, France
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Summary

Comparison of the primary sequences of bovine, ovine and caprine αs1-casein shows a deletion of eight amino acid residues in the ovine casein region 141–148, which is identical in the bovine and caprine proteins except for a single difference in position 148 (Q or E). Polyclonal antibodies raised in rabbits against the bovine casein sequence 140–149 (QELAYFYPEL) appeared monospecific for bovine αsl-casein, since no antibody-antigen complex was formed with homologous ovine or caprine proteins. These antibodies remained unable to recognize the caprine sequence in the native protein even after extensive tryptic proteolysis. The lack of immunoreactivity of the antibodies against synthetic caprine αsl-casein peptide 138–149 (VNQELAYFYPQL) suggested that the glutamic acid residue in position 148 is essential for the antigenic character of the bovine peptide. From these observations, the use of these antibodies for the detection and quantitation of bovine milk present in ovine dairy products could be extended to caprine products.

Type
Original articles
Information
Journal of Dairy Research , Volume 62 , Issue 1 , February 1995 , pp. 83 - 88
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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References

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