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Nitrogen source: an effective component for the growth and viability of Lactobacillus delbrueckii subsp. bulgaricus

Published online by Cambridge University Press:  30 August 2022

Raphael D. Ayivi ,
Salam A. Ibrahim ,
Albert Krastanov ,
Abishek Somani  and
Shahida A. Siddiqui
Raphael D. Ayivi
Affiliation:
Department of Food and Nutritional Sciences, North Carolina A&T State University, Greensboro, NC 27411, USA Joint School of Nanoscience and Nanoengineering, University of North Carolina, Greensboro, NC 27412, USA
Salam A. Ibrahim*
Affiliation:
Department of Food and Nutritional Sciences, North Carolina A&T State University, Greensboro, NC 27411, USA
Albert Krastanov
Affiliation:
Department of Biotechnology, University of Food Technologies, Plovdiv, Bulgaria
Abishek Somani
Affiliation:
Ohly Gmbh, Wandsbeker Zollstrasse 59, 22041 Hamburg, Germany
Shahida A. Siddiqui
Affiliation:
Department of Biotechnology and Sustainability, Technical University of Munich (TUM), 94315 Straubing, Germany DIL e.V.–German Institute of Food Technologies, 49610 D-Quakenbrück, Germany
*
Author for correspondence: Salam A. Ibrahim, Email: ibrah001@ncat.edu
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Abstract

In this study, we developed and optimized a growth media by evaluating various nitrogen sources for the cultivation of Lactobacillus bulgaricus, a probiotic and an important dairy starter culture. We modified the composition of deMan, Rogosa and Sharpe (MRS) culture media and substituted the nitrogen content with alternative nitrogen sources X-Seed KAT, X-Seed Carbo Max and X-Seed Nucleo Max in various blends of 5 g/l and 10 g/l respectively. Results showed that bacterial growth was significantly higher when the nitrogen source blend KCMax (10/10) was used. The optical density (OD610 nm) of the Lactobacillus bulgaricus strains were higher (1.34 and 1.79) in the KCMax (10/10) medium than in the MRS medium (0.89 and 1.42) (P < 0.05). There was no significant difference in the bacterial counts for both the MRS medium and the KCMax (10/10) medium, and all bacterial counts were estimated at 8 log CFU/ml. The buffering capacity of KCMax (10/10) was also tested and supplemented with l-histidine and was significantly different (P < 0.05) than that of the MRS control medium. Calcium supplemented in the KCMax (10/10) also served as a cryoprotectant for the cells during freezing and freeze-drying. Bacterial counts of the recovered calcium-treated freeze-dried cells were statistically significant (P < 0.05). We hypothesized that alternative nitrogen sources such as selected yeast extracts from the X-Seed brand of complex nitrogen sources could efficiently support the viability of Lb. bulgaricus. Our results thus suggested the growth of Lb. bulgaricus was efficiently supported by the X-Seed KAT, X-Seed Nucleo Max and X-Seed Carbo Max nitrogen sources. Consequently, these alternative nitrogen sources could potentially be recommended for dairy starter culture fermentations.

Keywords

Bacterial enumerationgrowth mediaLb. bulgaricusnitrogen sourcestarter culture
Type
Research Article
Information
Journal of Dairy Research , Volume 89 , Issue 3 , August 2022 , pp. 316 - 322
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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