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A novel method for species identification in milk and milk-based products

Published online by Cambridge University Press:  29 January 2008

Silvia Reale ,
Angela Campanella ,
Amalia Merigioli  and
Fabio Pilla
Silvia Reale*
Affiliation:
Dipartimento S.A.V.A., Università del Molise, Via de Sanctis snc86100 Campobasso, Italy Parco Scientifico e Tecnologico del Molise “Moliseinnovazione” S.C.a r.l., Via de Sanctis snc86100 Campobasso, Italy
Angela Campanella
Affiliation:
Dipartimento S.A.V.A., Università del Molise, Via de Sanctis snc86100 Campobasso, Italy
Amalia Merigioli
Affiliation:
Parco Scientifico e Tecnologico del Molise “Moliseinnovazione” S.C.a r.l., Via de Sanctis snc86100 Campobasso, Italy
Fabio Pilla
Affiliation:
Dipartimento S.A.V.A., Università del Molise, Via de Sanctis snc86100 Campobasso, Italy
*
*For correspondence; e-mail: reale@unimol.it
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Abstract

This study describes a method for species-specific detection of animal DNA from different species (cattle, sheep, goat, water buffalo) in milk and dairy products. A primer set was designed in conserved region on the basis of the alignment of the sequence codifying the genomic κ-casein gene in order to amplify all four species with a single primer pair. Polymorphisms were detected via minisequencing with extension primers designed in conserved sequences for haplotype determination that allow unambiguous assignment to each species. The method was successfully applied to the detection of raw and pasteurized milk from the four different species considered as well as to cheese products from the retail trade. Estimation of the limit of detection was carried out using a progression of dilutions of genomic DNA as well as DNA isolated from milk of a known number of somatic cells from different species in order to be able to achieve detection rates as low as 0·1% bovine milk mixed with buffalo milk.

Keywords

Species identificationfood traceabilitymilk
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 1 , February 2008 , pp. 107 - 112
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Addeo, F, Moio, L, Chianese, C, Stingo, C, Resini, P, Berner, I, Krause, I, Di Luccia, A & Bocca, A 1990 Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine cheese by gel isoelectric focusing of caseins. Milchwissenschaft 5 708–711Google Scholar
Bania, J, Ugorski, M, Polanowski, A & Adamczyk, E 2001 Application of polymerase chain reaction for detection of goats' milk adulteration by milk of cow. Journal of Dairy Research 68 333–336CrossRefGoogle ScholarPubMed
Beer, M, Krause, I, Stapf, M, Schwarzer, C & Klostermeyer, H 1996 Indirect competitive enzyme-linked immunosorbent assay for the detection of native and heat-denatured bovine β-lactoglobulin in ewes' and goats' milk cheese. European Food Research and Technology 203 21–26Google Scholar
Bottero, MT, Civera, T, Anastasio, A, Turi, RM & Rosati, S 2002 Identification of cows's milk in “buffalo” cheese by duplex polymerase chain reaction. Journal of Food Protection 65 362–366CrossRefGoogle ScholarPubMed
Bottero, MT, Civera, T, Nucera, D, Rosati, S, Sacchi, P & Turi, RM 2003 A multiplex polymerase chain reaction for the identification of cows', goats' and sheep's milk in dairy products. International Dairy Journal 13 277–282Google Scholar
Commission Regulation (E.C.) No. 213/01 of 9 January, 2001 lying down detailed rules for the application of Council Regulation (E.C.) No. 1255/1999 as regards methods for the analysis and quality evaluation of milk and milk products and amending Regulations (E.C.) No. 2771/1999 and (E.C.) No. 2799/1999. Official Journal of the European Communities, L037: 1–99Google Scholar
Feligini, M, Ionizzi, I, Curik, VC, Parma, P, Greppi, GF & Enne, G 2005 Detection of Adulteration in Mozzarella Cheese. Food Technology and Biotechnology. 43 91–95Google Scholar
Gazzetta Ufficiale della Repubblica Italiana (10 April, 1996) Decree of the Italian Minister of Agricultural, Food and Forestry Resources. Official Journal of the Italian Republic, 135 (11 June, 1996).Google Scholar
Hurley, P, Coleman, RC, Ireland, HE & Williams, JHH 2004 Measurement of Bovine IgG by Indirect Competitive ELISA as a Means of Detecting Milk Adulteration. Journal of Dairy Science 87 543–549Google Scholar
Lipkin, E, Shalom, A, Khatib, H, Soller, M & Friedmann, A 1993 Milk as a source of deoxyribonucleic acid and substrate for the polymerase chain reaction. Journal of Dairy Science 76 2025–2032Google Scholar
Mafra, I, Ferreira, I, Faria, MA & Oliveira, BPP 2004 A Novel Approach to the Quantification of Bovine Milk in Ovine Cheeses Using a Duplex Polymerase Chain Reaction Method. Journal of Agricultural Food Chemistry 52 4943–4947CrossRefGoogle Scholar
Matsunaga, T, Chikuni, K, Tanabe, B, Muroya, B, Shibata, K, Yamada, J & Shinmura, Y 1999 A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Science 51 143–148CrossRefGoogle ScholarPubMed
Maudet, C & Taberlet, P 2001 Detection of cow's milk in goat's cheeses inferred from mitochondrial DNA polymorphism. Journal of Dairy Research 68 229–235CrossRefGoogle ScholarPubMed
Mayer, HK, Heidler, D & Rockenbauer, C 1997 Determination of the Percentages of Cows', Ewes' and Goats' Milk in Cheese by Isoelectric Focusing and Cation-exchange HPLC of γ- and Para-κ-Caseins. International Dairy Journal 7 619–628Google Scholar
Moatsou, G, Hatzinaki, A, Psathas, G & Anifantakis, E 2004 Detection of caprine casein in ovine Halloumi cheese. International Dairy Journal 3 219–226Google Scholar
Moio, L, Sasso, ML, Chianese, L & Addeo, F 1990 Rapid detection of bovine milk in ovine, caprine and water buffalo milk or cheese by gel isoelectric focusing on phastsystem. Italian Journal of Food Science 3 185–190Google Scholar
Pellegrino, L, De Noni, I, Tirelli, A & Resmini, P 1991 Detection of cow milk in non-bovine cheese by HPLC of whey proteins. Scienze e. Tecnologie Lattiero Casearie 42 87Google Scholar
Plath, A, Krause, I & Einspanier, R 1997 Species identification in dairy products by three different DNA-based techniques. Zeitschrift für Lebensmitteluntersuchung und -Forschung 205 437–441CrossRefGoogle Scholar
Recio, I, García-Risco, MR, Amigo, L, Molina, E, Ramos, M & Martín-Álvarez, PJ 2004 Detection of Milk Mixtures in Halloumi Cheese. Journal of Dairy Science 87 1595–1600CrossRefGoogle ScholarPubMed
Rea, S, Chikuni, K, Branciari, R, Sangamaya, RS, Ranucci, D & Avellini, P 2001 Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese. Journal of Dairy Research 68 689–698CrossRefGoogle ScholarPubMed