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Oestradiol enhances apoptosis in bovine mammary epithelial cells in vitro

Published online by Cambridge University Press:  14 December 2012

Lucile Yart ,
Laurence Finot ,
Vanessa Lollivier  and
Frederic Dessauge
Lucile Yart
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France
Laurence Finot
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France
Vanessa Lollivier
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France Université européenne de Bretagne, France
Frederic Dessauge*
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France
*
*For correspondence; e-mail: frederic.dessauge@rennes.inra.fr
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Abstract

Ovarian steroids, oestradiol and progesterone, are required for normal mammary growth at puberty and during pregnancy. They contribute to mammary parenchyma development by stimulating mammary epithelial cell (MEC) proliferation. However several studies demonstrate that oestradiol negatively affects milk production during the declining phase of lactation, but the oestradiol effect on MEC in lactating mammary gland remains unclear. The objective of this study was to investigate the differential effect of oestradiol on bovine MECs mimicking two physiological statuses: active and early apoptotic MECs. We demonstrated that oestradiol has a major effect on early apoptotic MECs and might accelerate MEC apoptosis by activation of caspases rather than by inducing apoptosis in active MECs. Early apoptotic MECs could be compared with senescent cells in the late-lactation mammary gland. These results suggest that the negative effect of oestradiol on milk production during the declining phase of lactation would be due to an enhancement of apoptotic processes in MECs.

Keywords

Oestradiolmammary epithelial cellapoptosiscowMAC-T cell
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 1 , February 2013 , pp. 113 - 121
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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