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Pasteurized whole milk confers reduced susceptibilities to the antimicrobial agents trimethoprim, gatifloxacin, cefotaxime and tetracycline via the marRAB locus in Escherichia coli

Published online by Cambridge University Press:  07 November 2008

Yang Peng ,
Ricardo L Hernandez ,
Robert R Crow ,
Suzanna E Jones ,
Sara A Mathews ,
Ayanna M Arnold ,
Eliseo F Castillo ,
Jennifer M Moseley  and
Manuel F Varela
Yang Peng
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Ricardo L Hernandez
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Robert R Crow
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Suzanna E Jones
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Sara A Mathews
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Ayanna M Arnold
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Eliseo F Castillo
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Jennifer M Moseley
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
Manuel F Varela*
Affiliation:
Department of Biology, Eastern New Mexico University, Portales, New Mexico 88130USA
*
*For correspondence; e-mail: Manuel.Varela@enmu.edu
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Abstract

We inoculated pasteurized whole milk with Escherichia coli strains GC4468 (intact marRAB locus), JHC1096 (Δ marRAB), or AG112 (Δ marR), and incubated each overnight at 37°C. All strains were then recovered from the milk cultures, and susceptibilities to antimicrobial agents were determined by the E-test strip method (CLSI). Cells of strain GC4468, prior to culturing in milk, were susceptible to trimethoprim, gatifloxacin, cefotaxime and tetracycline. After culturing GC4468 in pasteurized milk, however, the minimal inhibitory concentrations (MICs) increased 1·4-fold for trimethoprim (P⩽0·05), 1·5-fold for gatifloxacin (P⩽0·05), 2·0-fold for cefotaxime (P=0·008), and 1·4-fold for tetracycline (P⩾0·05). After culturing GC4468 on milk count agar the MICs were enhanced 3·4-fold for trimethoprim (P⩽0·05), 10-fold for gatifloxacin (P=0·001), 7·1-fold for cefotaxime (P=0·011), and 40·5-fold for tetracycline (P=0·074), but exhibiting tetracycline resistance with a mean MIC of 74·7±18·47 μg/ml (CLSI). The MICs of the antimicrobial agents for JHC1096 cells after culturing in pasteurized whole milk were indistinguishable (P⩾0·05) from baseline MICs measured before culturing in the same type of milk. Thus, Esch. coli cells harbouring the marRAB locus exhibit reduced susceptibilities to multiple antimicrobial agents after culturing in pasteurized whole milk.

Keywords

Antimicrobial agentbacteriamilkmarRAB locussusceptibility
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 4 , November 2008 , pp. 491 - 496
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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