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Rapid method for cholesterol analysis in bovine milk and options for applications

Published online by Cambridge University Press:  23 November 2009

Enrique Viturro ,
Heinrich H Meyer ,
Carsten Gissel  and
Martin Kaske
Enrique Viturro*
Affiliation:
Physiology Weihenstephan, Technical University Munich, Germany
Heinrich H Meyer
Affiliation:
Physiology Weihenstephan, Technical University Munich, Germany
Carsten Gissel
Affiliation:
Gissel-Institut, Laboratory for Bacteriology and Food Hygiene, Hannover, Germany
Martin Kaske
Affiliation:
Clinic for Cattle, University of Veterinary Medicine, Hannover, Germany
*
*For correspondence; e-mail: viturro@wzw.tum.de
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Abstract

The adaptation of a colorimetric technique for the analysis of cholesterol in raw milk is presented. Performance quality was satisfying (mean intra-assay coefficient of variation (CV) 4·8, inter-assay CV 9·1%, linearity between 0 and 7 mm, recovery of spiked cholesterol into raw milk 98·1 and 106·3%). However, the milk fat extraction must be carried out within the 48 hours following milk sampling. When performing sampling, the significant variation of milk cholesterol composition during the milking process has to be taken into account.

Keywords

CholesterolAnalysisfat fractiondairy cow
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 1 , February 2010 , pp. 85 - 89
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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