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The reaction of lactose with anthrone and its application to the estimation of lactose in casein and other dairy products

Published online by Cambridge University Press:  01 June 2009

E. L. Richards
E. L. Richards
Affiliation:
The Dairy Research Institute (N.Z.), Palmerston North, New Zeland
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Extract

Lactose was estimated accurately and rapidly in casein by use of anthrone reagent on the clear filtrate from precipitation of a sodium bicarbonate solution of the casein with 0·1 n-sulphuric acid. The reagent consists of 0·15% (w/v) anthrone in 70% (v/v) sulphuric acid. The ice-cold anthrone solution (10 ml.) was added, with cooling, to 1 ml. of the ice-cold solution containing 0–100 µg. of lactose. The mixture was heated at 100° C. for 6 min. and cooled for 30 min. The optical density of the coloured solution was then measured at 625 mµ

Type
Original Articles
Information
Journal of Dairy Research , Volume 26 , Issue 1 , February 1959 , pp. 53 - 57
Copyright
Copyright © Proprietors of Journal of Dairy Research 1959

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References

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