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Risk factors of S. aureus intramammary infection in pre partum dairy heifers under grazing conditions and molecular characterization of isolates from heifers and cows

Published online by Cambridge University Press:  03 February 2020

Cecilia M. Camussone ,
Ana I. Molineri ,
Marcelo L. Signorini ,
Verónica E. Neder ,
Carlos A. Vitulich  and
Luis F. Calvinho
Cecilia M. Camussone*
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina EEA Rafaela, INTA, Santa Fe, Argentina
Ana I. Molineri
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina EEA Rafaela, INTA, Santa Fe, Argentina
Marcelo L. Signorini
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina EEA Rafaela, INTA, Santa Fe, Argentina
Verónica E. Neder
Affiliation:
EEA Rafaela, INTA, Santa Fe, Argentina
Carlos A. Vitulich
Affiliation:
EEA Rafaela, INTA, Santa Fe, Argentina
Luis F. Calvinho
Affiliation:
EEA Rafaela, INTA, Santa Fe, Argentina
*
Author for correspondence: Cecilia Camussone, Email: camussone.cecilia@inta.gob.ar
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Abstract

The aims of the research reported here were to identify potential risk factors associated with the presence of Staphylococcus aureus intramammary infection (IMI) in pre partum dairy heifers on 17 dairy farms from three provinces of Argentina and to characterize, at molecular level, isolates from those heifers and lactating cows from two selected herds. A total of 1474 heifers and 4878 lactating cows were studied. The prevalence of Staphylococcus aureus IMI in the heifers, heifers at quarter level and lactating cow mammary quarters was 14.41, 4.82, and 14.65%, respectively. Univariate analysis showed the key variables associated with S. aureus IMI presence in the heifers were: S. aureus IMI prevalence in cows of the lactating herd, the time calves stayed with their dam after birth, the calf rearing system, the place of rearing (own farm or other dairy farm) and fly control on the farm. None of the variables included in the multivariable analysis was associated with the presence of S. aureus IMI in the pre partum heifers, probably due to low variability among management practices used by the farms for rearing the heifer calves. At the molecular level, S. aureus isolates were grouped into three main PFGE clusters and several genotypes within the clusters. Isolates from mammary secretion of pre partum heifers and milk of lactating cows comprised different PFGE clusters in both herds, although two exceptions occurred. The absence of gene fnbpB, which codifies for a virulence factor protein involved in cell invasion by S. aureus, was significantly more frequent in pre partum heifer secretion isolates than in isolates from lactating cow milk. These results suggest that, under these management conditions, isolates from mammary secretions of pre partum heifers do not originate from the milk of lactating cows, but rather other sources to which the heifer is exposed.

Keywords

Heifer intramammary infectionheifersmolecular characterizationrisk factorsS. aureus
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 1 , February 2020 , pp. 82 - 88
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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References

Aarestrup, FM and Jensen, NE (1997) Prevalence and duration of intramammary infection in Danish heifers during the peripartum period. Journal of Dairy Science 80, 307–312.Google ScholarPubMed
Anderson, KL, Lyman, R, Moury, K, Ray, D, Watson, DW and Correa, MT (2012) Molecular epidemiology of Staphylococcus aureus mastitis in dairy heifers. Journal of Dairy Science 95, 4921–4930.Google ScholarPubMed
Barbagelata, MS, Alvarez, LP, Dotto, CM, Lattar, SM, Sordelli, DO and Buzzola, FR (2012) Aromatic compound-dependent Staphylococcus aureus is safe in a nasal colonization leukopenic murine model. International Journal of Microbiology 2012, 468539.Google Scholar
Bardiau, M, Caplin, J, Detilleux, J, Graber, H, Moroni, P, Taminiau, B and Mainil, JG (2016) Existence of two groups of Staphylococcus aureus strains isolated from bovine mastitis based on biofilm formation, intracellular survival, capsular profile and agr-typing. Veterinary Microbiology 185, 1–6.Google ScholarPubMed
Calvinho, LF, Canavesio, VR, Iguzquiza, IA, Marioni, I, Puricelli, FG, Neder, VE, Tarabla, HD and Aubagna, MD (2007) Intramammary infections during the periparturient period in Argentinean dairy heifers. Revista Argentina de Microbiología 39, 84–89.Google Scholar
Capurro, A, Aspán, A, Ericsson Unnerstad, H, Persson Waller, K and Artursson, K (2010) Identification of potential sources of Staphylococcus aureus in herds with mastitis problems. Journal of Dairy Science 93, 180–191.Google ScholarPubMed
Castelani, L, Silva Santos, AF, dos Santos Miranda, M, Zafalon, LF, Rodrigues Pozzi, C and Rodrigues Pozzi Arcaro, J (2013) Molecular typing of mastitis-causing Staphylococcus aureus isolated from heifers and cows. International Journal of Molecular Sciences 14, 4326–4333.Google ScholarPubMed
Chowdhury, S, Sandberg, M, Themudo, GE and Ersboll, KA (2012) Risk factors for Campylobacter infection in Danish broiler chickens. Poultry Science Association 91, 2701–2709.Google ScholarPubMed
Compton, CWR, Heuer, C, Parker, K and McDougall, S (2007) Epidemiology of mastitis in pasture-grazed peripartum dairy heifers and its effects on productivity. Journal of Dairy Science 90, 4157–4170.Google ScholarPubMed
De Vliegher, S, Fox, LK, Piepers, S, McDougall, S and Barkema, HW (2012) Mastitis in dairy heifers: nature of the disease, potential impact, prevention, and control. Journal of Dairy Science 95, 1025–1040.Google ScholarPubMed
Dieser, SA, Vissio, C, Lasagno, MC, Bogni, CI, Larriestra, AJ and Odierno, LM (2014) Prevalence of pathogens causing subclinical mastitis in Argentinean dairy herds. Pakistan Veterinary Journal 34, 124–126.Google Scholar
Dohoo, IR, Ducrot, C, Fourichon, C, Donald, A and Hurnik, D (1997) An overview of techniques for dealing with large numbers of independent variables in epidemiologic studies. Preventive Veterinary Medicine 29, 221–239.Google ScholarPubMed
Federation of Animal Sciences Societies (FASS) (2010) Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching, 3rd Edn. Champaign, Illinois, USA: FASS, p. 169.Google Scholar
Fox, LK (2009) Prevalence, incidence and risk factors of heifer mastitis. Veterinary Microbiology 134, 82–88.Google ScholarPubMed
Fox, LK, Chester, ST, Hallberg, JW, Nickerson, SC, Pankey, JW and Weaver, LD (1995) Survey of intramammary infections in dairy heifers at breeding age and first parturition. Journal of Dairy Science 78, 1619–1628.Google ScholarPubMed
Gillespie, BE, Owens, WE, Nickerson, SC and Oliver, SP (1999) Deoxyribonucleic acid fingerprinting of Staphylococcus aureus from heifer mammary secretions and from horn flies. Journal of Dairy Science 82, 1581–1585.Google ScholarPubMed
Haveri, M, Hovinen, M, Roslöf, A and Pyörälä, S (2008) Molecular types and genetic profiles of Staphylococcus aureus strains isolated from bovine intramammary infections and extramammary sites. Journal of Clinical Microbiology 46, 3728–3735.Google ScholarPubMed
Martineau, F, Picard, FJ, Roy, PH, Ouellette, M and Bergeron, MG (1998) Species specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. Journal of Clinical Microbiology 36, 618–623.Google ScholarPubMed
Matos, JS, White, DG, Harmon, RJ and Langlois, BE (1991) Isolation of Staphylococcus aureus from sites other than the lactating mammary gland. Journal of Dairy Science 74, 1544–1549.Google Scholar
Nyman, AK, Emanuelson, U, Gustafsson, AH and Persson Waller, K (2009) Management practices associated with udder health of first-parity dairy cows in early lactation. Preventive Veterinary Medicine 88, 138–149.Google ScholarPubMed
Oliver, SP, Gonzalez, RN, Hogan, JS, Jayarao, BM and Owens, WE (2004) Microbiological Procedures for the Diagnosis of Bovine Udder Infection and Determination of Milk Quality, 4th Edn. Verona, WI, USA: National Mastitis Council.Google Scholar
Oliver, SP, Gillespie, BE, Headrick, SJ, Lewis, MJ and Dowlen, HH (2005) Prevalence, risk factors, and strategies for controlling mastitis in heifers during the periparturient period. International Journal of Applied Research in Veterinary Medicine 3, 150–162.Google Scholar
Owens, WE, Oliver, SP, Gillespie, BE, Ray, CH and Nickerson, SC (1998) Role of horn flies (Haematobia irritans) in Staphylococcus-induced mastitis in dairy heifers. American Journal of Veterinary Research 59, 1122–1124.Google Scholar
Pereyra, EAL, Picech, F, Renna, MS, Baravalle, C, Andreotti, CS, Calvinho, LF, Diez, C and Dallard, BE (2016) Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells. Veterinary Microbiology 183, 69–77.Google ScholarPubMed
Persson, Y, Nyman, A and Grönlund-Andersson, U (2011) Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden. Acta Veterinaria Scandinavica 53, 36–43.Google ScholarPubMed
Reyher, KK and Dohoo, IR (2011) Diagnosing intramammary infections: evaluation of composite milk samples to detect intramammary infections. Journal of Dairy Science 94, 3387–3396.Google ScholarPubMed
Roberson, JR, Fox, LK, Hancock, OD, Gay, JM and Besser, TE (1994) Coagulase-positive Staphylococcus intramammary infections in primiparous dairy cows. Journal of Dairy Science 77, 958–969.Google ScholarPubMed
Roberson, JR, Fox, LK, Hancock, OD, Gay, JM and Besser, TE (1998) Sources of intramammary infections from Staphylococcus aureus in dairy heifers at first parturition. Journal of Dairy Science 81, 687–693.Google ScholarPubMed
Trinidad, P, Nickerson, SC and Alley, TK (1990) Prevalence of intramammary infection and teat canal colonization in unbred and primigravid dairy heifers. Journal of Dairy Science 73, 107–114.Google ScholarPubMed
Zecconi, A, Calvinho, L and Fox, L (2006) Staphylococcus aureus Intramammary Infections. Bulletin of the International Dairy Federation. 408/2006. Brussels: FIL-IDF, p. 39.Google Scholar
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