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Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm™ Aerobic Count plates and conventional pour plate methodology

Published online by Cambridge University Press:  09 July 2007

Maria BT Ortolani ,
Gabriela N Viçosa ,
Vanerli Beloti  and
Luís A Nero
Maria BT Ortolani
Affiliation:
Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, LondrinaPR, Brasil Departamento de Veterinária, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, ViçosaMG, Brasil
Gabriela N Viçosa
Affiliation:
Departamento de Veterinária, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, ViçosaMG, Brasil
Vanerli Beloti
Affiliation:
Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, LondrinaPR, Brasil
Luís A Nero*
Affiliation:
Departamento de Veterinária, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, ViçosaMG, Brasil
*
*For correspondence; e-mail: nero@ufv.br
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Abstract

This study aimed to compare Petrifilm™ Aerobic Count (AC) plates and the conventional pour plate methodology using de Mann-Rogosa-Sharpe (MRS), Kang-Fung (KF) and Kang-Fung-Sol (KFS) culture media for screening and enumeration of lactic acid bacteria (LAB) in milk. Suspensions of 10 LAB species in reconstituted powder skim milk and 30 raw milk samples, without experimental inoculation, were tested. For selective enumeration, all samples were previously diluted in MRS, KF and KFS broths and then plated in Petrifilm™ AC and conventional pour plate methodology, using the same culture media with added agar. All plates were incubated at 37°C for 48 h in anaerobic conditions. Differences in the counts were observed only for raw milk samples using KFS in conventional methodology, when compared with the counts obtained from MRS and KF (P⩽0·05). The results showed excellent correlation indexes between both methodologies using the three culture media for LAB suspensions (r=0·97 for MRS, KF and KFS). For raw milk samples, the correlation indexes were excellent (r=0·97, for MRS) and good (r=0·84 for KF, and r=0·82 for KFS), showing some interference in Petrifilm™ AC when supplements were added, especially lactic acid. These results indicate the possibility of using Petrifilm™ AC plates for enumeration of LAB in milk, even with the use of selective supplements.

Keywords

Lactic acid bacteriaPetrifilm™de Mann-Rogosa-SharpeKang-FungKang-Fung-Sol
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 4 , November 2007 , pp. 387 - 391
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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