Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T13:04:50.690Z Has data issue: false hasContentIssue false

Species identification of staphylococci and micrococci isolated from ewes' milk cheeses

Published online by Cambridge University Press:  01 June 2009

María Camino García
Affiliation:
Department of Food Hygiene and Food Microbiology, Veterinary Faculty, University of León, 24007-León, Spain
Andrés Otero
Affiliation:
Department of Food Hygiene and Food Microbiology, Veterinary Faculty, University of León, 24007-León, Spain
María Luisa García
Affiliation:
Department of Food Hygiene and Food Microbiology, Veterinary Faculty, University of León, 24007-León, Spain
María Rosario García
Affiliation:
Department of Food Hygiene and Food Microbiology, Veterinary Faculty, University of León, 24007-León, Spain
Benito Moreno
Affiliation:
Department of Food Hygiene and Food Microbiology, Veterinary Faculty, University of León, 24007-León, Spain

Summary

Forty-five strains of staphylococci and 6 strains of micrococci isolated from pasteurized ewes' milk cheeses (Manchego and Burgos varieties) were identified to species level. Three strains of staphylococci were identified as Staphylococcus aureus. Amongst the 42 coagulase-negative staphylococci, 8 were novobiocin-sensitive and 34 novobiocin-resistant. The novobiocin-sensitive species found were Staph. auricularis (5 strains), Staph. caseolyticus (2 strains) and Staph. epidermidis (1 strain). Novobiocin-resistant strains were identified as Staph. gallinarum (5 strains), Staph. xylosus (4), Staph. saprophyticus (9), Staph. cohnii (6), Staph. sciuri (1) and Staph. lentus (3). The other 6 isolates could not be identified. Only 3 strains of micrococci were identified (Micrococcus kristinae, M. varians and M. sedentarius). The possible origin of the isolates is discussed.

Type
Original articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anon. 1985 [Outbreaks of food infections and food intoxications in Spain, 1984.] Boletín Epidemiológico Semanal No 1687Google Scholar
Baird-Parker, A. C. 1979 Methods for identifying staphylococci and micrococci. In Identification Methods for Microbiologists, 2nd edn pp. 201210 (Eds. Skinner, F. A. and Lovelock, D. W.) London: Academic Press (Society for Applied Bacteriology Technical Series No. 14)Google Scholar
Barber, M. & Kuper, S. W. A. 1951 Identification of Staphylococcus pyogenes by the phosphatase reaction. Journal of Pathology and Bacteriology 63, 6568CrossRefGoogle ScholarPubMed
Blair, J. E. & Williams, R. E. O. 1961 Phage typing of staphylococci. Bulletin of the World Health Organization 24 771784Google ScholarPubMed
Cowan, S. T. 1974 Cowan & Steel's Manual for the Identification of Medical Bacteria, 2nd ednCambridge: Cambridge University PressGoogle Scholar
Devriese, L. A. & Hájek, V. 1980 Identification of pathogenic staphylococci isolated from animals and foods derived from animals: a review. Journal of Applied Bacteriology 49 111.CrossRefGoogle Scholar
Evans, J. B. & Kloos, W. E. 1972 Use of shake cultures in a semisolid thioglycolate medium for differentiating staphylococci from micrococci. Applied Microbiology 23, 326331CrossRefGoogle Scholar
García, M. C., García, M. L., Otero, A. & Moreno, B. 1988 Correlation between DNA base composition and routine test for the classification of Micrococcaceae isolated from sheep's milk cheese. Systematic and Applied Microbiology (in press)CrossRefGoogle Scholar
García, M. L., Francisco, J. J. & Moreno, B. 1986 Nasal carriage of Staphylococcus species by food handlers. International Journal of Food Microbiology 3 99108CrossRefGoogle Scholar
García, M. L., Moreno, B. & Bergdoll, M. S. 1980 Characterization of staphylococci isolated from mastitic cows in Spain. Applied and Environmental Microbiology 39 548553CrossRefGoogle ScholarPubMed
Gutiérrez, L. M., Menes, I., García, M. L., Moreno, B. & Bergdoll, M. S. 1982 Characterization and enterotoxigenicity of staphylococci isolated from mastitic ovine milk in Spain. Journal of Food Protection 45 12821286CrossRefGoogle Scholar
Hájek, V. 1978 Identification of enterotoxigenic staphylococci from sheep and sheep cheese. Applied and Environmental Microbiology 35 264268CrossRefGoogle ScholarPubMed
Ibrahim, G. F. 1981 A simple sensitive method for determining staphylococcal thermonuclease in cheese. Journal of Applied Bacteriology 51, 307312CrossRefGoogle ScholarPubMed
International Commission On Microbiological Specifications For Foods 1978 Microorganisms in Foods. I. Their significance and methods of enumeration, 2nd ednToronto: University of Toronto PressGoogle Scholar
Kloos, W. E. 1980 Natural populations of the genus Staphylococcus. Annual Review of Microbiology 34 559592CrossRefGoogle ScholarPubMed
Kloos, W. E. 1982 Coagulase-negative staphylococci. Clinical Microbiology Newsletter 4 7579CrossRefGoogle Scholar
Kloos, W. E. & Schleifer, K. H. 1975 Simplified scheme for routine identification of human Staphylococcus species. Journal of Clinical Microbiology 1 8288CrossRefGoogle ScholarPubMed
Kloos, W. E. & Schleifer, K. H. 1983 Staphylococcus auricularis sp.nov.: an inhabitant of the human external ear. International Journal of Systematic Bacteriology 33 914CrossRefGoogle Scholar
Kloos, W. E. & Schleifer, K. H. 1986 Genus IV. Staphylococcus. In Bergey's Manual of Systematic Bacteriology vol. 2, pp. 10131035 (Ed. Sneath, P. H. A.) Baltimore, MD: Williams & WilkinsGoogle Scholar
Kloos, W. E., Tornabene, T. G. & Schleifer, K. H. 1974 Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae. International Journal of Systematic Bacteriology 24 79101CrossRefGoogle Scholar
Kloos, W. E., Zimmerman, R. J. & Smith, R. F. 1976 Preliminary studies on the characterization and distribution of Staphylococcus and Micrococcus species on animal skin. Applied and Environmental Microbiology 31 5359CrossRefGoogle ScholarPubMed
Kocur, M. 1986 Genus I. Micrococcus. In Bergey's Manual of Systematic Bacteriology vol. 2, pp. 10041008 (Ed. Sneath, P. H. A.) Baltimore, MD: Williams & WilkinsGoogle Scholar
Lenoir, J. 1984 The surface flora and its role in the ripening of cheese. International Dairy Federation Bulletin Document No 171 320Google Scholar
National Research Council 1985 An Evaluation of the Role of Microbiological Criteria for Foods and Food Ingredients. Washington, DC: National Academy PressGoogle Scholar
Nünez, M. & Medina, M. 1980 [Micrococci and staphylococci of Cabrales blue cheese.] Lait 60 171183Google Scholar
Nurmi, E. 1966 Effect of bacterial inoculation on characteristics and microbial flora of dry sausage. Acta Agralia Fennica 108 177Google Scholar
Parker, M. T. 1972 Phage typing of Staphylococcus aureus. In Methods in Microbiology vol. 7B, pp. 128 (Eds. Norris, J. R. and Ribbons, D. W.) London: Academic PressGoogle Scholar
Robbins, R., Gould, S. & Bergdoll, M. S. 1974 Detecting the enterotoxigenicity of Staphylococcus aureus strains. Applied Microbiology 28 946950CrossRefGoogle ScholarPubMed
Saxe, M. De, Coe, A. W. & Wieneke, A. A. 1982 The use of phage typing in the investigation of food poisoning caused by Staphylococcus aureus enterotoxins. In Isolation and Identification Methods for Food Poisoning Organisms pp. 173197 (Eds Corry, J. E. L., Roberts, D. and Skinner, F. A.) London: Academic Press (Society for Applied Bacteriology Technical Series No. 17)Google Scholar
Schleifer, K. H. & Fischer, U. 1982 Description of a new species of the genus Staphylococcus: Staphylococcus carnosus. International Journal of Systematic Bacteriology 32 153156CrossRefGoogle Scholar
Schleifer, K. H., Kilpper-Bälz, R. & Devriese, L.-A. 1984 Staphylococcus arlettae sp.nov., S. equorum sp.nov. and S. kloosii sp.nov.: three new coagulase-negative, novobiocin-resistant species from animals. Systematic and Applied Microbiology 5 501509CrossRefGoogle Scholar
Schleifer, K. H., Kilpper-Bälz, R., Fischer, U., Faller, A. & Endl, J. 1982 Identification of Micrococcus candidus' ATCC 14852 as a strain of Staphylococcus epidermidis and of ‘Micrococcus caseolyticus’ ATCC 13548 and Micrococcus varians ATTC 29750 as members of a new species, Staphylococcus caseolyticus. International Journal of Systematic Bacteriology 32 1520CrossRefGoogle Scholar
Schleifer, K. H. & Kloos, W. E. 1975 A simple test system for the separation of staphylococci from micrococci. Journal of Clinical Microbiology 1 337338CrossRefGoogle ScholarPubMed
Smith, R. F. & Willett, N. P. 1968 Rapid plate method for screening hyaluronidase and chondroitin sulfatase-producing microorganisms. Applied Microbiology 16 14341436CrossRefGoogle ScholarPubMed
Speck, M. L. (Ed.) 1976 Compendium of Methods for the Microbiological Examination of Foods. Washington DC: American Public Health AssociationGoogle Scholar
Speck, M. L. (Ed.) 1984 Compendium of Methods for the Microbiological Examination of Foods, 2nd ednWashington DC: American Public Health AssociationGoogle Scholar
Subcommittee on Phage Typing of Staphylococci 1984 Report of the Subcommittee on Phage Typing of Staphylococci to the International Committee on Systematic Bacteriology. International Journal of Systematic Bacteriology 34 260261Google Scholar
Subcommittee on Taxonomy of Staphylococci and Micrococci 1965 Minutes of first meeting. International Bulletin of Bacteriological Nomenclature and Taxonomy 15 107108Google Scholar
Turner, F. J. & Schwartz, B. S. 1958 The use of a lyophilized human plasma standardized for blood coagulation factors in the coagulase and fibrinolytic tests. Journal of Laboratory and Clinical Medicine 52 888894Google ScholarPubMed