Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-28T04:04:08.743Z Has data issue: false hasContentIssue false
  • English
  • Français

Staphylococcus aureus strains in primiparous and multiparous cows in six herds with a high prevalence of Staph. aureus intramammary infections

Published online by Cambridge University Press:  26 July 2007

Bernd-Alois Tenhagen ,
Nicole Scheibe ,
Bert-Andree Zucker ,
Gudrun Köster  and
Wolfgang Heuwieser
Bernd-Alois Tenhagen*
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin
Nicole Scheibe
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin
Bert-Andree Zucker
Affiliation:
Institut für Tier- und Umwelthygiene, Luisenstraße 56, D-10117 Berlin
Gudrun Köster
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin
Wolfgang Heuwieser*
Affiliation:
Freie Universität Berlin, Tierklinik für Fortpflanzung, Königsweg 65, Hs. 27, D-14163 Berlin
*
†For correspondence; e-mail: author@bestandsbetreuung.de
†For correspondence; e-mail: author@bestandsbetreuung.de
Get access Rights & Permissions [Opens in a new window]

Abstract

The proportion of different strains of Staphylococcus aureus was tested in four groups of lactating dairy cows in six herds with a high overall prevalence of Staph. aureus using random amplified polymorphic DNA PCR. Group 1 included primiparous cows in early lactation (<50 days in milk, DIM). Group 2 consisted of primiparous cows in late lactation (>250 days in milk). Groups 3 and 4 were multiparous cows in the respective stages of lactation. Eight cows from each group on each farm were tested. Overall quarter prevalence of Staph. aureus ranged from 23·4 to 32·0% in the herds. Of the 130 isolates included in the analysis 86·9% were high prevalence strains (more than three isolates per herd), while 13·1% were strains that were only identified in one or two samples. Low prevalence strains were found in all six herds. The proportion of low prevalence strains was higher in multiparous than in primiparous cows (odds ratio, OR 4·4, 1·2–16·6). It is concluded that low prevalence Staph. aureus strains are common even in herds with a high prevalence of Staph. aureus and that their frequency is lower in primiparous cows than in older cows.

Keywords

Staphylococcus aureusintramammary infectionmastitisdairy cow
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 4 , November 2007 , pp. 406 - 411
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Edinger, D, Tenhagen, BA, Kalbe, P, Klünder, G, Baumgärtner, B & Heuwieser, W 2000 Effect of teat dipping with a germicide barrier teat dip in late gestation on intramammary infection and clinical mastitis during the first five days post partum in primiparous cows. Journal of Veterinary Medicine A 47 463–468CrossRefGoogle ScholarPubMed
Gianneechini, R, Concha, C, River, R, Delucci, I & Moreno Lopez, J 2002 Occurrence of clinical and subclinical mastitis in dairy herds in the west littoral region in Uruguay. Acta Veterinaria Scandinavia 43 221–230CrossRefGoogle ScholarPubMed
Gillespie, BE, Owens, WE, Nickerson, SC & Oliver, SP 1999 Deoxyribonucleic acid fingerprinting of Staphylococcus aureus from heifer mammary secretions and from horn flies. Journal of Dairy Science 82 1581–1585Google Scholar
Grundmann, H, Hori, S, Enright, MC, Webster, C, Tami, A, Feil, EJ & Pitt, T 2002 Determining the genetic structure of the natural population of Staphylococcus aureus: a comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing. Journal of Clinical Microbiology 40 4544–4546.Google Scholar
Hoedemaker, M 2001 New aspects of combatting Staphylococcus aureus mastitis. Tierärztliche Praxis 29 (G) 1–7Google Scholar
Köster, G, Tenhagen, BA, Scheibe, N & Heuwieser, W 2006: Factors associated with high milk test day somatic cell counts in large dairy herds in Brandenburg. II: Milking practices. Journal of Veterinary Medicine A 53 209–214CrossRefGoogle ScholarPubMed
Lam, TJGM, Lipman, LJA, Schukken, YH, Gaastra, W & Brand, A 1996 Epidemiological characteristics of bovine clinical mastitis caused by Escherichia coli and Staphylococcus aureus studied by DNA fingerprinting. American Journal of Veterinary Research 57 39–42CrossRefGoogle ScholarPubMed
Lee, JH 2003 Methicillin (Oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Applied Environmental Microbiology 69 6489–6494CrossRefGoogle ScholarPubMed
Makovec, JA & Ruegg, PL 2003 Results of milk samples submitted for microbiological examination in Wisconsin from 1994 to 2001. Journal of Dairy Science 86 3466–3472Google Scholar
Neave, FK, Dodd, FH, Kingwill, RG & Westgarth, DR 1969 Control of mastitis in the dairy herd by hygiene and management. Journal of Dairy Science 52 696–707CrossRefGoogle ScholarPubMed
Østeras, O, Solverod, L, & Reksen, O 2006 Milk culture results in a large Norwegian survey – effects of season, parity, days in milk, resistance, and clustering. Journal of Dairy Science 89 1010–1023CrossRefGoogle Scholar
Poelarends, JJ, Hogeveen, H, Sampimon, OC & Sol, J 2001 Monitoring subclinical mastitis in Dutch dairy herds. In Proceedings of the 2nd International Symposium on Mastitis and Milk Quality. National Mastitis Council and American Association of Bovine Practioners Madison WI, USA and Rome, pp 145–149Google Scholar
Reppel, C, Falkenberg, U, Jung, M & Heuwieser, W 2005 Comparison of the strains of Staphylococcus aureus between dairy cows and heifers at time of calving. Proceedings of the 44th Annual Meeting of the National Mastitis Council, Orlando FL, USA, pp 283–284Google Scholar
Roberson, JR, Fox, LK, Hancock, DD & Besser, TE 1992 Evaluation of methods for differentiation of coagulase-positive staphylococci. Journal of Clinical Microbiology 30 3217–3219CrossRefGoogle ScholarPubMed
Roberson, JR, Fox, LK, Hancock, DD, Gay, JM & Besser, TE 1998 Sources of intramammary infections from Staphylococcus aureus in dairy heifers at first parturition. Journal of Dairy Science 81 687–693Google Scholar
Sabat, A, Malachowa, N, Miedzobrodzki, J & Hryniewicz, W 2006 Comparison of PCR-based methods for typing Staphylococcus aureus isolates. Journal of Clinical Microbiology 44 3804–3807Google Scholar
Sabour, PM, Gill, Lepp D, Pacan, JC, Ahmed, R, Dingwell, R & Leslie, K 2004 Molecular typing and distribution of Staphylococcus aureus isolates in eastern Canadian dairy herds. Journal of Clinical Microbiology 42 3449–3455Google Scholar
Smyth, DS, Kennedy, Twhig J, Miajlovic, H, Bolton, D & Smyth, CJ 2006 Staphylococcus aureus isolates from Irish domestic refrigerators possess novel enterotoxin and enterotoxin-like genes and are clonal in nature. Journal of Food Protection 69 508–515Google Scholar
Sommerhäuser, J, Kloppert, B, Wolter, W, Zschöck, M, Sobiraj, A & Failing, K 2003 The epidemiology of Staphylococcus aureus infection from subclinical mastitis in dairy cows during a control programme. Veterinary Microbiology 96 91–102Google Scholar
Tenhagen, BA, Edinger, D, Baumgärtner, B, Kalbe, P, Klünder, G & Heuwieser, W 2001 Efficacy of a herd specific vaccine against Staphylococcus aureus to prevent post-partum mastitis in dairy heifers. Journal of Veterinary Medicine A 48 601–608CrossRefGoogle ScholarPubMed
Tenhagen, BA, Köster, G, Wallmann, J & Heuwieser, W 2006: Prevalence of mastitis pathogens in dairy cows in Brandenburg, Germany, and their resistance against antimicrobial agents. Journal of Dairy Science 89 2542–2551Google Scholar
Zadoks, RN, Allore, HG, Barkema, HW, Sampimon, OC, Wellenberg, GJ, Gröhn, YT & Schukken, YH 2001 Cow- and quarter-level risk factors for Streptococcus uberis and Staphylococcus aureus mastitis. Journal of Dairy Science 84 2649–2663CrossRefGoogle ScholarPubMed