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Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway

Published online by Cambridge University Press:  07 April 2022

Mengzhu Qi ,
Hao Geng ,
Na Geng ,
Yukun Cui ,
Changxi Qi ,
Guodong Cheng ,
Kaimin Song ,
Liping Hu ,
Yongxia Liu  and
Jianzhu Liu
...Show all authors
Mengzhu Qi
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Hao Geng
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Na Geng
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Yukun Cui
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Changxi Qi
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Guodong Cheng
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Kaimin Song
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Liping Hu
Affiliation:
Shandong Provincial Center for Animal Disease Control and Prevention, Jinan, Shandong 251000, China
Yongxia Liu*
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China Research Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai`an, Shandong 271018, China
Jianzhu Liu*
Affiliation:
College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China
Bo Han
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
*
Authors for correspondence: Yongxia Liu, Email: liuyongxia@sdau.edu.cn Jianzhu Liu, Email: liujz@sdau.edu.cn
Authors for correspondence: Yongxia Liu, Email: liuyongxia@sdau.edu.cn Jianzhu Liu, Email: liujz@sdau.edu.cn
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Abstract

Streptococcus agalactiae (S. agalactiae) infection is a significant cause of mastitis, resulting in loss of cellular homeostasis and tissue damage. Autophagy plays an essential function in cell survival, defense, and the preservation of cellular homeostasis, and is often part of the response to pathogenic challenge. However, the effect of autophagy induced by S. agalactiae in bovine mammary epithelial cells (bMECs) is mainly unknown. So in this study, an intracellular S. agalactiae infection model was established. Through evaluating the autophagy-related indicators, we observed that after S. agalactiae infection, a significant quantity of LC3-I was converted to LC3-II, p62 was degraded, and levels of Beclin1 and Bcl2 increased significantly in bMECs, indicating that S. agalactiae induced autophagy. The increase in levels of LAMP2 and LysoTracker Deep Red fluorescent spots indicated that lysosomes had participated in the degradation of autophagic contents. After autophagy was activated by rapamycin (Rapa), the amount of p-Akt and p-mTOR decreased significantly, whilst the amount of intracellular S. agalactiae increased significantly. Whereas the autophagy was inhibited by 3-methyladenine (3MA), the number of intracellular pathogens decreased. In conclusion, the results demonstrated that S. agalactiae could induce autophagy through PI3K/Akt/mTOR pathway and utilize autophagy to survive in bMECs.

Keywords

Autophagybovine mammary epithelial cellintracellular survivalmastitisStreptococcus agalactiae
Type
Research Article
Information
Journal of Dairy Research , Volume 89 , Issue 2 , May 2022 , pp. 178 - 184
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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