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Tryptic phosphopeptides from whole casein. II. Physicochemical properties related to the solubilization of calcium

Published online by Cambridge University Press:  01 June 2009

Rafael Berrocal ,
Serge Chanton ,
Marcel A. Juillerat ,
Blaise Favillare ,
Jean-Claude Scherz  and
Rolf Jost
Rafael Berrocal
Affiliation:
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland
Serge Chanton
Affiliation:
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland
Marcel A. Juillerat
Affiliation:
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland
Blaise Favillare
Affiliation:
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland
Jean-Claude Scherz
Affiliation:
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland
Rolf Jost
Affiliation:
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland
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Summary

Casein phosphopeptides (GPP) were produced by tryptic hydrolysis of sodium caseinate and further purified by precipitation and chromatography on QAE-Sephadex A-25. Their physico-chemical properties were compared with the properties of an enzymically dephosphorylated equivalent preparation (DPP). Binding of Ca2+ to the peptides was measured using a Ca selective electrode and was found to increase with pH and to show 1/1 stoicheiometry Ca/Porg in CPP at pH 6·5 a.nd 7·6. Klotz plots indicated equivalent binding sites at these two pH values, but some heterogeneity was seen at pH 3·5. In contrast, DPP did not bind significant amounts of Ca2+.

CPP effectively inhibited the formation of insoluble calcium phosphates at different Ca/P ratios. The effective CPP concentration was 10 mg/1 and complete stability of calcium phosphate solutions was obtained at about 100 mg/1. This stabilizing effect was dependent on the presence of organic P.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 3: Special Issue: Proceedings of the Hannah Research Institute Casein Conference , May 1989 , pp. 335 - 341
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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References

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