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Ultrasonication and the quality of human milk: variation of power and time of exposure

Published online by Cambridge University Press:  05 July 2012

Lukas Christen ,
Ching Tat Lai  and
Peter E. Hartmann
Lukas Christen*
Affiliation:
School of Chemistry and Biochemistry, Faculty of Life and Physical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia Carag AG, Bahnhofstrasse 9, CH-6340 Baar, Switzerland
Ching Tat Lai
Affiliation:
School of Chemistry and Biochemistry, Faculty of Life and Physical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia
Peter E. Hartmann
Affiliation:
School of Chemistry and Biochemistry, Faculty of Life and Physical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia
*
*For correspondence; e-mail: 20795312@student.uwa.edu.au
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Abstract

Donor human milk is pasteurized to prevent the potential risk of the transmission of pathogens to preterm infants. Currently, Holder pasteurization (human milk held at 62·5°C for 30 min) is used in most human milk banks, but has the disadvantage that it results in excessive inactivation of important bioactive components. Power-ultrasound (20–100 kHz) is an emerging technology for the preservation of foods and could be an alternative method for the treatment of human milk. The aim of this study was to investigate the effect of different ultrasound settings on the elimination of Escherichia coli and the retention of bile salt stimulated lipase (BSSL) activity. Ultrasonication with a constant power decreased Esch. coli viability exponentially over time until the processing temperature increased to sub-pasteurization level to between 51·4 and 58·5°C, then a log10 1·3 decrease was observed (P<0·05). BSSL activity decreased to 91% until a temperature of 51·4°C and then it decreased to 8% between 51·4 and 64·9°C. Ultrasonication with a constant energy and various power and exposure times showed the highest temperature (53·7°C) when treated with the longest exposure time and lowest ultrasound-power (276 s at 3·62 W) compared with 37·6°C for 39 s at 25·64 W. The findings predict that the viability of Esch. coli could be reduced by log10 5 with a minimal loss of activity of BSSL by applying 13·8 kJ of energy in 12 ml of human milk using high ultrasound power over a short exposure time to ensure that the temperature remains below the critical level for protein denaturation. Alternatively, the use of lower power settings such as the 26 W used in the present studies would require a cooling system to ensure the human milk BSSL was protected against temperature denaturation.

Keywords

Donor human milkholder pasteurizationultrasonicationEsch. colibile salt stimulated lipase
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 3 , August 2012 , pp. 361 - 366
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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