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Variation in the yak lipin-1 gene and its association with milk traits

Published online by Cambridge University Press:  17 January 2020

Xin Wang ,
Huitong Zhou ,
Jon G. H. Hickford ,
Shaobin Li ,
Jiqing Wang ,
Xiu Liu ,
Jiang Hu  and
Yuzhu Luo
Xin Wang
Affiliation:
Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Huitong Zhou
Affiliation:
Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand International Science and Technology Cooperation Base of Meat Sheep and Meat Cattle Genetic Improvement in Northwest of China, Gansu Agricultural University, Lanzhou730070, China
Jon G. H. Hickford
Affiliation:
Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand International Science and Technology Cooperation Base of Meat Sheep and Meat Cattle Genetic Improvement in Northwest of China, Gansu Agricultural University, Lanzhou730070, China
Shaobin Li
Affiliation:
Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Jiqing Wang
Affiliation:
Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Xiu Liu
Affiliation:
Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Jiang Hu*
Affiliation:
Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Yuzhu Luo*
Affiliation:
Faculty of Animal Science and Technology, Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln7647, New Zealand
*
Authors for correspondence: Jiang Hu, Email huj@gsau.edu.cn and Yuzhu Luo, luoyz@gsau.edu.cn
Authors for correspondence: Jiang Hu, Email huj@gsau.edu.cn and Yuzhu Luo, luoyz@gsau.edu.cn
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Abstract

The aim of this research was to identify variation in the yak lipin-1 gene (LPIN1) and determine whether this variation affects milk traits. PCR-single stranded conformational polymorphism (PCR-SSCP) analysis was used to detect variation in the 5′ untranslated region of LPIN1 in 500 yaks from four populations: Tianzhu white yaks, Qinghai yaks, wild × domestic-cross yaks and Gannan yaks. Four unique PCR-SSCP patterns, representing four different DNA sequence variants (named A, B, C and D), were observed. These contained six single nucleotide polymorphisms. Female Gannan yaks with BC genotype produced milk with a higher fat content (P < 0.001) and total milk solids (P < 0.001), than those with the AA, AB and BB genotypes. These results would suggest that LPIN1 is having an effect on yak milk fat synthesis.

Keywords

LPIN1milk traitspolymorphismyak
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 166 - 169
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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