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X-ray diffraction has limited applicability in investigation of milk tampering

Published online by Cambridge University Press:  06 August 2019

Ana Paula Pavão Battaglini ,
Alexandre Urbano ,
Vanerli Beloti ,
Edson Antonio Rios ,
Juliana Ramos Pereira  and
Rafael Fagnani
Ana Paula Pavão Battaglini
Affiliation:
Department of Preventive Veterinary Medicine, Londrina State University, Londrina, Brazil
Alexandre Urbano
Affiliation:
Department of Physics, Londrina State University, Londrina, Brazil
Vanerli Beloti
Affiliation:
Department of Preventive Veterinary Medicine, Londrina State University, Londrina, Brazil
Edson Antonio Rios
Affiliation:
Department of Preventive Veterinary Medicine, Londrina State University, Londrina, Brazil
Juliana Ramos Pereira
Affiliation:
Department of Preventive Veterinary Medicine, Londrina State University, Londrina, Brazil
Rafael Fagnani*
Affiliation:
Department of Preventive Veterinary Medicine, Londrina State University, Londrina, Brazil
*
Author for correspondence: Rafael Fagnani, Email: rafaelfagnani@hotmail.com
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Abstract

The aim of this work was to use X-ray diffraction to identify substances used for adulteration of raw milk and to determine if crystallographic analysis can detect extraneous substances in milk. Two unknown substances were sent anonymously by employers linked to the dairy chain, who claimed that they were added directly in milk prior to water addition by truck drivers. The samples were analyzed by X-ray diffraction and submitted to physicochemical analysis. The first substance was identified by X-ray diffraction as sodium citrate, complying with its physicochemical attributes, such as the powerful ability to decrease the freezing point. The second substance was identified by X-ray diffraction as sucrose and this result was also in agreement with its ability to increase the density, decrease the freezing point and finally, to be positive for sucrose in the resorcinol qualitative test. To evaluate if X-ray diffraction can detect extraneous substances already mixed in milk, fresh raw milk samples tampered with urea, sodium hydroxide, sodium citrate and sucrose were freeze dried and analyzed by X-ray diffraction, with no detection of any extraneous substances at any percentage. This is the first report of attempted diagnosis of extraneous substances in milk by X-ray diffraction. However, this technique can be useful only when applied to identify substances used for adulteration prior to its dilution in milk, since the amorphous nature of milk seems to be a limitation for the accurate detection of extraneous substances.

Keywords

Citratefraudmilk adulterationsucrosewater
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 3 , August 2019 , pp. 337 - 340
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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References

Azad, T and Ahmed, S (2016) Common milk adulteration and their detection techniques. International Journal of Food Contamination 22, 1–9.Google Scholar
Brasil (2006) Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa no. 68 de 12 de dezembro de 2006. Diário Oficial da União, Brasília, Distrito Federal (In Portuguese).Google Scholar
Botelho, BG, Reis, N, Oliveira, LS and Sena, MM (2015) Development and analytical validation of a screening method for simultaneous detection of five adulterants in raw milk using mid-infrared spectroscopy and PLS-DA. Food Chemistry 181, 31–37.Google Scholar
Bugeat, S, Perez, J, Briard-Bion, V, Pradel, P, Ferlay, A, Bourgaux, C and Lopez, C (2015) Unsaturated fatty acid enriched vs. control milk triacylglycerols: solid and liquid TAG phases examined by synchrotron radiation X-ray diffraction coupled with DSC. Food Research International 67, 91–101.Google Scholar
Bunaciu, AA, UdriŞTioiu, EG and Aboul-Enein, HY (2015) X-ray diffraction: instrumentation and applications. Critical Reviews in Analytical Chemistry 45, 289–299.Google Scholar
Fagnani, R, Battaglini, APP, Beloti, V, Urbano, A and de Camargo Bronzol, J (2016) Alcohol stability of milk from the perspective of x-ray diffractometry. Food Biophysics 11, 198–205.Google Scholar
Handford, CE, Campbell, K and Elliott, CT (2016) Impacts of milk fraud on food safety and nutrition with special emphasis on developing countries. Comprehensive Reviews in Food Science and Food Safety 15, 130–142.Google Scholar
Masum, AKM, Chandrapala, J, Adhikari, B, Huppertz, T and Zisu, B (2019) Effect of lactose-to-maltodextrin ratio on emulsion stability and physicochemical properties of spray-dried infant milk formula powders. Journal of Food Engineering 254, 34–41.Google Scholar
Nascimento, CF, Santos, PM, Pereira-Filho, ER and Rocha, FR (2017) Recent advances on determination of milk adulterants. Food Chemistry 221, 1232–v1244.Google Scholar
Nijdam, J, Ibach, A, Eichhorn, K and Kind, M (2007) An X-ray diffraction analysis of crystallised whey and whey-permeate powders. Carbohydrate Research 342, 2354–2364.Google Scholar
Poonia, A, Jha, A, Sharma, R, Singh, HB, Rai, AK and Sharma, N (2017) Detection of adulteration in milk: a review. International Journal of Dairy Technology 70, 23–42.Google Scholar
Singh, P and Gandhi, N (2015) Milk preservatives and adulterants: processing, regulatory and safety issues. Food Reviews International 31, 236–261.Google Scholar
Thachepan, S, Li, M and Mann, S (2010) Mesoscale crystallization of calcium phosphate nanostructures in protein (casein) micelles. Nanoscale 2, 2400–2405.Google Scholar