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Maternal high-fat diet induces follicular atresia but does not affect fertility in adult rabbit offspring

Published online by Cambridge University Press:  11 February 2014

Pauline Léveillé ,
Anne Tarrade ,
Charlotte Dupont ,
Thibaut Larcher ,
Michèle Dahirel ,
Elodie Poumerol ,
Ann-Gaël Cordier ,
Olivier Picone ,
Béatrice Mandon-Pepin  and
Geneviève Jolivet
...Show all authors
Pauline Léveillé
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France Université Paris 13, Sorbonne Paris Cité, Unité de Recherche en Epidémiologie Nutritionnelle (UREN), F-93000, Bobigny, France APHP, Hôpital Jean-Verdier, F-93140,Bondy, France
Anne Tarrade
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France PremUp, 4 av. de l’Observatoire, F-75006 Paris, France
Charlotte Dupont
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France Université Paris 13, Sorbonne Paris Cité, Unité de Recherche en Epidémiologie Nutritionnelle (UREN), F-93000, Bobigny, France APHP, Hôpital Jean-Verdier, F-93140,Bondy, France
Thibaut Larcher
Affiliation:
INRA, UMR 703 APEX, Oniris, F-44307 Nantes, France
Michèle Dahirel
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France PremUp, 4 av. de l’Observatoire, F-75006 Paris, France
Elodie Poumerol
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France
Ann-Gaël Cordier
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France APHP, Hôpital Antoine Béclère, Service de Gynécologie-Obstétrique et Médecine de la Reproduction, F-92141, Clamart, France
Olivier Picone
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France
Béatrice Mandon-Pepin
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France
Geneviève Jolivet
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France
Rachel Lévy
Affiliation:
Université Paris 13, Sorbonne Paris Cité, Unité de Recherche en Epidémiologie Nutritionnelle (UREN), F-93000, Bobigny, France APHP, Hôpital Jean-Verdier, F-93140,Bondy, France
Pascale Chavatte-Palmer*
Affiliation:
INRA, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France ENVA, F-94700 Maisons Alfort, France PremUp, 4 av. de l’Observatoire, F-75006 Paris, France
*
*Address for correspondence: Pascale Chavatte-Palmer, UMR1198 Biologie du Développement et Reproduction, F-78350 Jouy-en-Josas, France. (Email pascale.chavatte@jouy.inra.fr)
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Abstract

Alterations to the metabolic environment in utero can have an impact on subsequent female reproductive performance. Here, we used a model of rabbits receiving a high-fat diet (H diet; 7.7% fat and 0.2% cholesterol) or a control diet (C diet; 1.8% fat, no cholesterol) from 10 weeks of age up to mating at 27 weeks and throughout gestation and lactation. At weaning at 5 weeks of age, F1 female offspring were placed on either C or H diet, resulting in a total of four groups C/C, C/H, H/C and H/H diet. Female offspring were mated between 18 and 22 weeks of age and euthanized at 28 days of gestation. A few days before mating and/or just before euthanasia, F1 female rabbits were fasted overnight, weighed, and blood sampled for steroids and biochemistry. Organs were weighed at euthanasia and the ovaries were collected. C/H and H/H F1 offspring had higher cholesterol and high-density lipoprotein plasma concentrations, together with a higher fat mass compared with C/C does, reflecting the effect of the postnatal diet; however, no effect of the antenatal diet was observed on most parameters. The number of primordial, primary and secondary follicles were not different between the groups, but a significantly higher number of atretic follicles was observed in the C/H (P<0.001) and in the H/C (P<0.001) compared with control C/C ovaries, demonstrating both an effect of prenatal and postnatal maternal nutrition. These data indicated that both maternal and postnatal high-fat diet may induce follicular apoptosis; however, in this model, the reproduction was not affected.

Keywords

follicleshigh-fat dietovaryrabbitreproduction
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 5 , Issue 2 , April 2014 , pp. 88 - 97
Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2014 

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