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Maternal intake of alpha-lipoic acid prevents development of symptoms associated with a fructose-rich diet in the male offspring in Wistar rats

Published online by Cambridge University Press:  11 December 2020

María Belén Rabaglino Open the ORCID record for María Belén Rabaglino [Opens in a new window] ,
María José Moreira-Espinoza Open the ORCID record for María José Moreira-Espinoza [Opens in a new window] ,
Clarisa Lagares ,
Maria Isabel Garay ,
Patricia Quiroga ,
María Eugenia Pasqualini ,
Flavio Francini  and
Dante Beltramo
María Belén Rabaglino*
Affiliation:
Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET). Pabellón Biología Celular, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina
María José Moreira-Espinoza
Affiliation:
Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET). Pabellón Biología Celular, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina Instituto de Biología Celular (IBC-UNC), Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina
Clarisa Lagares
Affiliation:
Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET). Pabellón Biología Celular, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina
Maria Isabel Garay
Affiliation:
Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET). Pabellón Biología Celular, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina Instituto de Biología Celular (IBC-UNC), Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina
Patricia Quiroga
Affiliation:
Instituto de Biología Celular (IBC-UNC), Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina
María Eugenia Pasqualini
Affiliation:
Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET). Pabellón Biología Celular, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina Instituto de Biología Celular (IBC-UNC), Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina
Flavio Francini
Affiliation:
Centro de Endocrinología Experimental y Aplicada (CENEXA), UNLP-CONICET-FCM, 1900La Plata, Argentina
Dante Beltramo
Affiliation:
CE.PRO.COR, CONICET, Av. Alvarez de Arenales 180, 5004Córdoba, Argentina
*
Address for correspondence: Maria Belen Rabaglino, DVM, MSc, PhD., Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Pabellón Biología Celular, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000Córdoba, Argentina. Email: belenrabaglino@icloud.com
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Abstract

The hypothesis was that maternal intake of the antioxidant alpha-lipoid acid (ALA), during the developmental period of the hypothalamic orexigenic neurons, causes a permanent beneficial effect in offspring metabolism. Pregnant Wistar rats were fed with standard diet (food) + ALA (0.4% wt/wt) from day 14 of gestation to day 20 of lactation (n = 4) or food (n = 4). At 3 months of age, male offspring born from ALA-fed rats or controls (CT) were randomly assigned to be fed with food + 10% fructose solution in drinking water (F) or food + tap water (C), resulting in four groups: ALAF, ALAC, CTF, and CTC (n = 5/group). Food intake and body weight (BW) were measured twice a week for 31 days. Metabolites’ levels in blood, mRNA expressions of Npy, Agrp (hypothalamus), Fasn, Srebf1, Ppard, and Pparg (liver), and the antioxidant capacity of the liver were determined. Results significance was set at p < 0.05. Average BW gain, daily BW gain, and intraabdominal fat tissue at necropsy were higher in CTF group followed by CTC, ALAF, and ALAC groups. There were no differences between groups in Kcal intake per day. mRNA expressions of hypothalamic and hepatic genes and plasmatic levels of glucose and triglycerides were higher in CTF group followed by ALAF, CTC, and ALAC groups. Fructose intake affected the oxidative capacity of the liver, but this effect was not observed in the ALAF group. In conclusion, maternal ALA intake protected the adult offspring to develop metabolic symptoms associated with high fructose in the drinking water.

Keywords

Fetal programmingmetabolic syndromemetabolismantioxidants
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 12 , Issue 5 , October 2021 , pp. 758 - 767
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Copyright
© The Author(s), 2020. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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