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Differential methylation of insulin-like growth factor 2 in offspring of physically active pregnant women

Part of: Liggins Institute, New Zealand and the Japanese DOHaD Society

Published online by Cambridge University Press:  09 January 2018

M. R. Marshall ,
N. Paneth ,
J. A. Gerlach ,
L. M. Mudd ,
L. Biery ,
D. P. Ferguson  and
J. M. Pivarnik
M. R. Marshall*
Affiliation:
Department of Kinesiology, Samford University, Birmingham, AL, USA
N. Paneth
Affiliation:
Department of Epidemiology & Biostatistics, Michigan State University, East Lansing, MI, USA
J. A. Gerlach
Affiliation:
Biomedical Laboratory Diagnostics Program, Michigan State University, East Lansing, MI, USA
L. M. Mudd
Affiliation:
National Institutes of Health, National Center for Complementary and Integrative Health, Bethesda, MD, USA
L. Biery
Affiliation:
Department of Epidemiology & Biostatistics, Michigan State University, East Lansing, MI, USA
D. P. Ferguson
Affiliation:
Department of Kinesiology, Michigan State University, East Lansing, MI, USA
J. M. Pivarnik
Affiliation:
Department of Epidemiology & Biostatistics, Michigan State University, East Lansing, MI, USA Department of Kinesiology, Michigan State University, East Lansing, MI, USA
*
Author for correspondence: M. R. Marshall, Department of Kinesiology, Samford University, 800 Lakeshore Dr., Birmingham, AL 35229, USA. E-mail mmarshal@samford.edu
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Abstract

Several studies have suggested that maternal lifestyle during pregnancy may influence long-term health of offspring by altering the offspring epigenome. Whether maternal leisure-time physical activity (LTPA) during pregnancy might have this effect is unknown. The purpose of this study was to determine the relationship between maternal LTPA during pregnancy and offspring DNA methylation. Participants were recruited from the Archive for Research on Child Health study. At enrollment, participants’ demographic information and self-reported LTPA during pregnancy were determined. High active participants (averaged 637.5 min per week of LTPA; n=14) were matched by age and race to low active participants (averaged 59.5 min per week LTPA; n=28). Blood spots were obtained at birth. Pyrosequencing was used to determine methylation levels of long interspersed nucleotide elements (LINE-1) (global methylation) and peroxisome proliferator-activated receptor-gamma (PPARγ), peroxisome proliferator-activated receptor-gamma coactivator (PGC1-α), insulin-like growth factor 2 (IGF2), pyruvate dehydrogenase kinase, isozyme 4 (PDK4) and transcription factor 7-like 2 (TCF7L2). We found no differences between offspring of high active and low active groups for LINE-1 methylation. The only differences in candidate gene methylation between groups were at two CpG sites in the P2 promoter of IGF2; the offspring of low active group had significantly higher DNA methylation (74.70±2.25% methylation for low active v. 72.83±2.85% methylation for high active; P=0.045). Our results suggest no effect of maternal LTPA on offspring global and candidate gene methylation, with the exception of IGF2. IGF2 has been previously associated with regulation of physical activity, suggesting a possible role of maternal LTPA on regulation of offspring physical activity.

Keywords

epigeneticsIGF2maternalmethylationphysical activitypregnancy
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 9 , Issue 3: Themed Issue: NZ-Japan , June 2018 , pp. 299 - 306
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2018 

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