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Effect of a low-protein diet during pregnancy on expression of genes involved in cardiac hypertrophy in fetal and adult mouse offspring

Published online by Cambridge University Press:  09 November 2010

S. Asopa ,
F. R. Cagampang ,
F. W. Anthony ,
S. A. Lanham ,
J. E. Schneider ,
S. K. Ohri  and
M. A. Hanson
S. Asopa
Affiliation:
Developmental Origins of Health and Disease Division, Institute of Developmental Sciences, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK
F. R. Cagampang*
Affiliation:
Developmental Origins of Health and Disease Division, Institute of Developmental Sciences, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK
F. W. Anthony
Affiliation:
Developmental Origins of Health and Disease Division, Institute of Developmental Sciences, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK
S. A. Lanham
Affiliation:
Developmental Origins of Health and Disease Division, Institute of Developmental Sciences, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK
J. E. Schneider
Affiliation:
Department of Cardiovascular Medicine, British Heart Foundation Molecular Cardiology Laboratory, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
S. K. Ohri
Affiliation:
Wessex Cardiothoracic Centre, Southampton General Hospital, Southampton, UK
M. A. Hanson
Affiliation:
Developmental Origins of Health and Disease Division, Institute of Developmental Sciences, University of Southampton School of Medicine, Southampton General Hospital, Southampton, UK
*
*Address for correspondence: F. R. Cagampang, Developmental Origins of Health and Disease Division, Institute of Developmental Sciences, University of Southampton School of Medicine, Southampton General Hospital, Mailpoint 887, Southampton SO16 6YD, UK. (Email f.cagampang@soton.ac.uk)
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Abstract

Gene markers for cardiomyocyte growth, proliferation and remodeling were examined in mouse fetuses and adult male offspring exposed to maternal low-protein (LP) diet during pregnancy. Whole heart volume, measured by magnetic resonance imaging, was smaller in day 15 LP fetuses v. those from chow-fed dams (C), whereas heart volume was greater in adult LP v. C offspring. These LP offspring were hypertensive and had larger cardiomyocytes v. C animals. The mRNA levels of cyclin G1, a marker for cell growth, were lower in LP fetal hearts v. C hearts, but similar in the left ventricle of adult LP and C offspring. Opposite trends were found in brain natriuretic peptide levels (a marker of cardiac hypertrophy). Thus, maternal LP during pregnancy results in smaller fetal hearts and is accompanied by changes in expression of genes involved in cardiomyocyte growth, which are associated with cardiac hypertrophy and hypertension in adulthood.

Keywords

BNPcardiac hypertrophycell cycle regulationcyclin G1MRI
Type
Brief Report
Information
Journal of Developmental Origins of Health and Disease , Volume 1 , Issue 6 , December 2010 , pp. 371 - 375
Copyright
Copyright © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2010

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