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Glyphosate-based herbicide exposure during pregnancy and lactation malprograms the male reproductive morphofunction in F1 offspring

Part of: DOHaD on International Women's Day 2020

Published online by Cambridge University Press:  16 July 2019

Jakeline Liara Teleken ,
Ellen Carolina Zawoski Gomes ,
Carine Marmentini ,
Milara Bruna Moi ,
Rosane Aparecida Ribeiro ,
Sandra Lucinei Balbo ,
Elaine Manoela Porto Amorim  and
Maria Lúcia Bonfleur Open the ORCID record for Maria Lúcia Bonfleur [Opens in a new window]
Jakeline Liara Teleken
Affiliation:
Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil
Ellen Carolina Zawoski Gomes
Affiliation:
Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil
Carine Marmentini
Affiliation:
Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil
Milara Bruna Moi
Affiliation:
Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil
Rosane Aparecida Ribeiro
Affiliation:
Laboratório Integrado de Morfologia, NUPEM, Universidade Federal do Rio de Janeiro (UFRJ), Campus UFRJ-Macaé, Macaé, RJ, Brazil
Sandra Lucinei Balbo
Affiliation:
Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil
Elaine Manoela Porto Amorim
Affiliation:
Laboratório de Histologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil
Maria Lúcia Bonfleur*
Affiliation:
Laboratório de Fisiologia Endócrina e Metabolismo (LAFEM), Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Cascavel, Brazil
*
Address for correspondence: Maria Lúcia Bonfleur, Laboratório de Fisiologia Endócrina e Metabolismo, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, CEP 85819-110, Brazil. Email: mlbonfleur@hotmail.com
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Abstract

One of the most consumed pesticides in the world is glyphosate, the active ingredient in the herbicide ROUNDUP®. Studies demonstrate that glyphosate can act as an endocrine disruptor and that exposure to this substance at critical periods in the developmental period may program the fetus to induce reproductive damage in adulthood. Our hypothesis is that maternal exposure to glyphosate during pregnancy and lactation in mice will affect the development of male reproductive organs, impairing male fertility during adult life. Female mice consumed 0.5% glyphosate-ROUNDUP® in their drinking water [glyphosate-based herbicide (GBH) group] or filtered water [control (CTRL) group] from the fourth day of pregnancy until the end of the lactation period. Male F1 offspring were designated, according to their mother’s treatment, as CTRL-F1 and GBH-F1. Female mice that drank glyphosate displayed reduced body weight (BW) gain during gestation, but no alterations in litter size. Although GBH male F1 offspring did not exhibit modifications in BW, they demonstrated delayed testicular descent. Furthermore, at PND150, GBH-F1 mice presented a lower number of spermatozoa in the cauda epididymis and reduced epithelial height of the seminiferous epithelium. Notably, intratesticular testosterone concentrations were enhanced in GBH-F1 mice; we show that it is an effect associated with increased plasma and pituitary concentrations of luteinizing hormone. Therefore, data indicate that maternal exposure to glyphosate-ROUNDUP® during pregnancy and lactation may lead to decreased spermatogenesis and disruptions in hypothalamus–pituitary–testicular axis regulation in F1 offspring.

Keywords

Developmental origins of health and diseaseendocrine disruptor chemicalhypothalamus–pituitary–testicular axispesticidesspermatozoatestosterone
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 11 , Issue 2 , April 2020 , pp. 146 - 153
Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2019

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