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Hitting a triple in the non-alcoholic fatty liver disease field: sucrose intake in adulthood increases fat content in the female but not in the male rat offspring of dams fed a gestational low-protein diet

Published online by Cambridge University Press:  18 December 2017

L. Nicolás-Toledo ,
M. Cervantes-Rodríguez ,
E. Cuevas-Romero ,
D. L. Corona-Quintanilla ,
E. Pérez-Sánchez ,
E. Zambrano ,
F. Castelán  and
J. Rodríguez-Antolín Open the ORCID record for J. Rodríguez-Antolín [Opens in a new window]
L. Nicolás-Toledo
Affiliation:
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
M. Cervantes-Rodríguez
Affiliation:
Licenciatura en Nutrición, Facultad de Ciencias de la Salud, Universidad Autónoma de Tlaxcala, Tlaxcala, México
E. Cuevas-Romero
Affiliation:
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
D. L. Corona-Quintanilla
Affiliation:
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
E. Pérez-Sánchez
Affiliation:
Maestría en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México Licenciatura en Nutrición, Universidad de la Sierra Sur, Miahuatlán, Oaxaca, México
E. Zambrano
Affiliation:
Departamento de Biología de la Reproducción, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México-CDMX, México
F. Castelán
Affiliation:
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Tlaxcala, México
J. Rodríguez-Antolín*
Affiliation:
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México
*
*Address for correspondence: J. Rodríguez Antolín, Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala 90000, México. (Email jorge.rodrigueza@uatx.mx; antolin26@gmail.com)
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Abstract

The excessive consumption of carbohydrates is related to non-alcoholic fatty liver disease (NAFLD) in infants and adults. The effect of combining maternal malnutrition and a high carbohydrate intake on the development of NAFLD in adulthood remains unknown. We therefore hypothesized that consumption of 5% sucrose by the offspring of dams fed a low-protein diet during pregnancy promotes liver fat accumulation and oxidative damage differently in females and males. To test this, 12-month-old female and male offspring of mothers fed a Control (C) or low-protein diet (Restricted, R) were provided with either tap water or 5% sucrose for a period of 10 weeks. Livers were excised to measure the fat content and 3-nitrotyrosine (3-NTyr) immunostaining; serum samples were also obtained to measure the concentration of malondialdehyde (MDA). Data were analyzed using a non-repeated measures three-way analysis of variance to determine significant differences (P<0.05) regarding to the interaction among maternal diet, sucrose consumption and sex. Results showed that the liver fat content of females from R mothers was higher than that of their male counterpart. Hepatic 3-NTyr immunostaining and serum MDA concentrations were not affected by the interaction involving maternal diet, sucrose consumption and sex. Otherwise, liver fat content was correlated with the hepatic 3-NTyr immunostaining and serum MDA concentrations only in females. Thus, sucrose intake in adulthood increases fat content in the female but not in the male rat offspring of dams fed with a low-protein diet during pregnancy. This research emphasizes the importance of a balanced diet during pregnancy and the influence of the diet on the adult offspring.

Keywords

lipid peroxidationmaternal malnutritionNAFLDsucrosetriglycerides
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 9 , Issue 2 , April 2018 , pp. 151 - 159
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2017 

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