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A paternal hypercaloric diet affects the metabolism and fertility of F1 and F2 Wistar rat generations

Published online by Cambridge University Press:  15 January 2020

Leonardo Toshio Oshio Open the ORCID record for Leonardo Toshio Oshio [Opens in a new window] ,
Ana Eliza Andreazzi ,
Jéssica Ferraz Lopes Open the ORCID record for Jéssica Ferraz Lopes [Opens in a new window] ,
Jackson Pereira de Sá ,
Mariana Bolotari ,
Vinícius Moreira Gonçalves Costa Open the ORCID record for Vinícius Moreira Gonçalves Costa [Opens in a new window] ,
Martha de Oliveira Guerra  and
Vera Maria Peters
Leonardo Toshio Oshio*
Affiliation:
Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Ana Eliza Andreazzi
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Jéssica Ferraz Lopes
Affiliation:
Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Jackson Pereira de Sá
Affiliation:
Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Mariana Bolotari
Affiliation:
Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Vinícius Moreira Gonçalves Costa
Affiliation:
Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Martha de Oliveira Guerra
Affiliation:
Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
Vera Maria Peters
Affiliation:
Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil
*
Address for correspondence: Leonardo Oshio, Centro de Biologia da Reprodução, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. Email: leonardo.oshio@gmail.com
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Abstract

Increased fat and carbohydrate intakes based on the Western diet are important lifestyle modifications that lead to hypercaloric inputs, obesity, and male fertility negative effects. Epigenetic transmission may also predispose descended generations to chronic diseases, such as obesity, type 2 diabetes, behavioral, and reproductive disorders. The present study sought to evaluate the influence of a high-fat-high-sugar (HFHS) diet supplied to Wistar rats from 25 to 90 days of life on reproductive and metabolic parameters in male generations F0, F1, and F2. The standard group received the normocaloric – Nuvilab Quimtia® –3.86 kcal/kg. The hypercaloric diet (HD) group received the HFHS diet – PragSoluções® –4.77 kcal/kg. Body weight, adiposity, F1 and F2 prepubertal age evaluations, oral glucose tolerance test, insulin tolerance test, organ weights, sperm count and morphology assessments, and histometric testicular analyses were performed. The HFHS diet promoted dyslipidemia, higher adiposity, lower relative organ weights, and higher mean kidney weight, decreased mean testicle and parenchyma weights and lower height of seminiferous epithelium (HE) for the F0 generation. F1 and F2 offspring of HD group displayed early preprepubertal development, although did not alter the metabolic parameters. Decreased HE and tubular testicular compartment volumetric density and increased intertubular testicular compartment volumetric density and volume in the F1 generation of HD group were observed. Alterations in histometry of intertubular testicular compartment were also noted. It is concluded that the HFHS experimental model altered only paternal metabolic parameters. However, reproductive parameters of the three generations were affected.

Keywords

Hypercaloric dietmale infertilityratstestesepigenetic
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 11 , Issue 6 , December 2020 , pp. 653 - 663
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2020

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