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Perturbations of gut microbiota in gestational diabetes mellitus patients induce hyperglycemia in germ-free mice

Part of: 2019 DOHaD 11th World Congress

Published online by Cambridge University Press:  14 September 2020

Yu Liu ,
Shengtang Qin ,
Ye Feng ,
Yilin Song ,
Na Lv ,
Fei Liu ,
Xiaoming Zhang ,
Shuxian Wang ,
Yumei Wei  and
Shuang Li
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Yu Liu
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Shengtang Qin
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Ye Feng
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Yilin Song
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Na Lv
Affiliation:
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Fei Liu
Affiliation:
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Xiaoming Zhang
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Shuxian Wang
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Yumei Wei
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Shuang Li
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Shiping Su
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Wanyi Zhang
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Yong Xue
Affiliation:
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Yanan Hao
Affiliation:
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Baoli Zhu*
Affiliation:
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Jingmei Ma*
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
Huixia Yang*
Affiliation:
Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing, China Beijing Key Laboratory of Maternal Foetal Medicine of Gestational Diabetes Mellitus, Beijing, China Peking University, Beijing, China
*
Address for correspondence: Baoli Zhu, Email: zhubaoli@im.ac.cn; Jingmei Ma, Email: jingmeima@bjmu.edu.cn; Huixia Yang, Email: yanghuixia@bjmu.edu.cn
Address for correspondence: Baoli Zhu, Email: zhubaoli@im.ac.cn; Jingmei Ma, Email: jingmeima@bjmu.edu.cn; Huixia Yang, Email: yanghuixia@bjmu.edu.cn
Address for correspondence: Baoli Zhu, Email: zhubaoli@im.ac.cn; Jingmei Ma, Email: jingmeima@bjmu.edu.cn; Huixia Yang, Email: yanghuixia@bjmu.edu.cn
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Abstract

Shifts in the maternal gut microbiota have been implicated in the development of gestational diabetes mellitus (GDM). Understanding the interaction between gut microbiota and host glucose metabolism will provide a new target of prediction and treatment. In this nested case-control study, we aimed to investigate the causal effects of gut microbiota from GDM patients on the glucose metabolism of germ-free (GF) mice. Stool and peripheral blood samples, as well as clinical information, were collected from 45 GDM patients and 45 healthy controls (matched by age and prepregnancy body mass index (BMI)) in the first and second trimester. Gut microbiota profiles were explored by next-generation sequencing of the 16S rRNA gene, and inflammatory factors in peripheral blood were analyzed by enzyme-linked immunosorbent assay. Fecal samples from GDM and non-GDM donors were transferred to GF mice. The gut microbiota of women with GDM showed reduced richness, specifically decreased Bacteroides and Akkermansia, as well as increased Faecalibacterium. The relative abundance of Akkermansia was negatively associated with blood glucose levels, and the relative abundance of Faecalibacterium was positively related to inflammatory factor concentrations. The transfer of fecal microbiota from GDM and non-GDM donors to GF mice resulted in different gut microbiota colonization patterns, and hyperglycemia was induced in mice that received GDM donor microbiota. These results suggested that the shifting pattern of gut microbiota in GDM patients contributed to disease pathogenesis.

Keywords

Gestational diabetes mellitus (GDM)gut microbiotagerm-free micefecal microbiota transplantation
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 11 , Issue 6 , December 2020 , pp. 580 - 588
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Copyright
© The Author(s), 2020. Published by Cambridge University Press in association with the International Society for Developmental Origins of Health and Disease

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