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Placental mitochondrial biogenesis and function was slightly changed by gestational hypercholesterolemia in full-term pregnant women

Part of: French DOHaD

Published online by Cambridge University Press:  04 March 2018

Z.-Y. Le ,
S. Dong ,
R. Zhang ,
X.-P. Cai ,
A. Gao ,
R. Xiao  and
H.-L. Yu
Z.-Y. Le
Affiliation:
School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China
S. Dong
Affiliation:
School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China
R. Zhang
Affiliation:
School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China
X.-P. Cai
Affiliation:
School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China
A. Gao
Affiliation:
School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China
R. Xiao
Affiliation:
School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China
H.-L. Yu*
Affiliation:
School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China
*
Address for correspondence: H.-L. Yu, School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China. E-mail: yuhlzjl@ccmu.edu.cn
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Abstract

It was reported that high blood cholesterol levels increased the susceptibility to mitochondrial dysfunction. This study hypothesized that the gestational hypercholesterolemia (HC) could induce the mitochondrial dysfunction in term human placenta. The eligible pregnant women were recruited from Xuanwu Hospital in Beijing during their first prenatal visit (before their 10th week of pregnancy). In total, 19 pregnant women whose serum total cholesterol levels were higher than 7.25 mm at third trimester (measured at 36–38 weeks) were selected as gestational HC. Other 19 pregnant women with normal cholesterol level matched with age, pre-gestational body mass index, and the neonatal gender were included as the control group. Full-term placenta samples were collected. The mitochondrial DNA (mtDNA) copy number, messenger RNA (mRNA) expression of cytochrome c oxidase subunit I, adenosine triphosphate monophosphatase 6 (ATP6ase), citrate synthase, peroxisome proliferator-activated receptor-γ (PPARγ) co-activator 1α, PPARγ co-activator 1β and estrogen-related receptor-α, and the activity of mitochondrial respiratory chain enzyme complex were measured. Pregnancy outcomes were obtained by extraction from medical records and the labor ward register. The results showed that only placental mtDNA copy number and mRNA expression of ATP6ase were significantly decreased in HC group. No significant differences were detected of other measurements between the two groups. These findings indicated that gestational HC might not induce the damage of placental function seriously.

Keywords

activity of respiratory chain enzymegestational hypercholesterolemiamitochondrionplacenta
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 9 , Issue 4: Themed Issue: French DOHaD , August 2018 , pp. 395 - 400
Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2018 

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