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Differentiation between Trichinella spiralis and T. pseudospiralis infective larvae by a monoclonal antibody

Published online by Cambridge University Press:  05 June 2009

J. Rodriguez-Perez ,
V. Gomez-Garcia ,
M. Rodriguez-Osorio ,
J. Rojas-Gonzalez  and
M. A. Gomez-Morales
J. Rodriguez-Perez
Affiliation:
Instituto “López-Neyra” de Parasitología, Unidad de Inmunología, C/ Ventanilla, 11, 18001-Granada, Spain
V. Gomez-Garcia
Affiliation:
Instituto “López-Neyra” de Parasitología, Unidad de Inmunología, C/ Ventanilla, 11, 18001-Granada, Spain
M. Rodriguez-Osorio
Affiliation:
Instituto “López-Neyra” de Parasitología, Unidad de Inmunología, C/ Ventanilla, 11, 18001-Granada, Spain
J. Rojas-Gonzalez
Affiliation:
Instituto “López-Neyra” de Parasitología, Unidad de Inmunología, C/ Ventanilla, 11, 18001-Granada, Spain
M. A. Gomez-Morales
Affiliation:
Instituto “López-Neyra” de Parasitología, Unidad de Inmunología, C/ Ventanilla, 11, 18001-Granada, Spain
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Abstract

Crude saline extracts of Trichinella spiralis and T. pseudospiralis infective larvae were studied by Western blot analysis using a monoclonal antibody, named ES/TA2 and produced against T. spiralis larvae. This monoclonal antibody recognized seven major antigenic components in T. spiralis larvae with apparent Mr: 45, 48, 50, 68, 70, 92 and 105 kDa and five in T. pseudospiralis larvae: 38, 50, 70, 72 and 92 kDa. SDS-PAGE of both extracts did not reveal appreciable differences in the range of molecular weights recognized by ES/TA2. These facts show the existence of immunological differences among proteins with apparently identical molecular weights.

Keywords

Trichinella spiralisTrichinella pseudospiralismonoclonal antibodieschemotaxonomy
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 4 , December 1989 , pp. 275 - 279
Copyright
Copyright © Cambridge University Press 1989

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